Influence of Ultrasonic Treatment on the Allergenic Properties of Shrimp （Penaeus vannamei ） Allergen     

LI Zhenxing LIN Hong CAO Limin 《中国海洋大学学报(英文版)》2006,5(2):115-118

The present study was undertaken to determine whether high intensity ultrasound could reduce the allergic properties of shrimp allergens. Reducing the allergenic properties of these allergens will be beneficial to allergic individuals. Samples of shrimp protein extract and shrimp muscle were treated by high-intensity ultrasound with water bathing at 0 ℃ or 50 ℃ for different time periods. The treated and untreated samples were then analyzed by SDS-PAGE, Western blots and competitive inhibition ELISA （Ci-ELISA） to determine the shrimp allergenicity. The results show that high-intensity ultrasound has no effect on allergenicity when the extracts were treated at 0℃. However, a significant decrease was observed in the level of the major shrimp allergen, Pen a 1, when the samples were treated at 50 ℃. In the determination of allergenicity with CiELISA, a reduction in IgE binding was also observed.  相似文献   

Numerical simulation of three-dimensional tidal current in the Bobal Sea     

Den Zhenxing  Yang Lianwu  Jose Ozer 《海洋学报(英文版)》1994,13(2):155-172

Ｎｕｍｅｒｉｃａｌｓｉｍｕｌａｔｉｏｎｏｆｔｈｒｅｅ－ｄｉｍｅｎｓｉｏｎａｌｔｉｄａｌｃｕｒｒｅｎｔｉｎｔｈｅＢｏｂａｌＳｅａ￥ＤｅｎＺｈｅｎｘｉｎｇ;ＹａｎｇＬｉａｎｗｕ;ａｎｄＪｏｓｅＯｚｅｒ（ＲｅｃｅｉｖｅｄＭａｙ１,１９９３;ａｃｃｅｐｔｅｄ...  相似文献   

3种鲍16S rRNA基因片段序列的初步研究   总被引：13，自引：0，他引：13  

杨建敏  郑小东  王如才  宋志乐  孙振兴 《中国海洋大学学报(自然科学版)》2003,33(1):36-40

本文对引自日本的盘鲍 (H aliotisdiscusdiscus)、皱纹盘鲍 (H.discushannai)和大鲍 (H.gi-gantiea) 3个自然群体的线粒体 DNA 1 6S r RNA基因片段进行了扩增和测序 ,分析了 52 8bp的碱基序列 ,结果显示 :3种鲍的基因序列中 A+T含量为 56.74%～ 57.1 2 %。 3个自然群体间碱基片段序列差异不显著 ,盘鲍与皱纹盘鲍、大鲍彼此均仅有 1处核苷酸检测到变异 ,皆为碱基转换 ,同源性为99.81 % ;皱纹盘鲍与大鲍有 2处碱基转换 ,同源性为 99.61 %。 1 6Sr RNA基因在鲍属内表现出很高的保守性。  相似文献   

皱纹盘鲍三倍体生长的初步研究   总被引：13，自引：0，他引：13  

孙振兴  宋志乐 《海洋湖沼通报》1992,(4):70-75

将用低温休克法和细胞松弛素B诱导法,获得的皱纹盘鲍(Haliotis discus hannai Ino)三倍体诱导群与二倍体的个体,在室内同样条件下饲育,对其生长情况进行了比较,结果表明:(1)三倍体与二倍体的当年稚鲍在壳长和体重的增长上无明显差异。(2)三倍体与二倍体的2龄鲍,在第二年前期生长无明显差异,但从第二年后期开始,三倍体的壳长、体重增长逐渐优于二倍体。(3)统计学分析表明,三倍体与二倍体的3龄鲍在体重、软体部和足肌的增长方面,存在着明显差异。(4)在室内饲育19个月的鲍,三倍体诱导群的壳长比二倍体大10.2％,体重比二倍体大20.1％,足肌湿重比二倍体大17.6％。  相似文献   

四种重金属对刺参幼参的急性致毒效应   总被引：1，自引：0，他引：1  

孙振兴  陈书秀  陈静  高洪梅  李丹 《海洋通报》2007,26(5):80-85

采用静水试验法,在水温18.5℃~20℃的条件下,用Cu2 ,Zn2 ,Cd2 和Cr6 四种重金属对刺参幼参进行了单一急性毒性试验。结果表明:Cu2 对刺参幼参的24h,48h,72h和96hLC50分别为0.299,0.176,0.133和0.120mg/L;Zn2 的24h,48h,72h和96hLC50分别为6.700,3.624,2.577和1.951mg/L;Cd2 的24h,48h和72hLC50分别为4.246,2.588和2.137mg/L;Cr6 的24h,48h和72hLC50分别为31.974,7.499和3.808mg/L;Cu2 ,Zn2 ,Cd2 和Cr6 对刺参幼参的安全浓度分别为0.018,0.362,0.259和0.750mg/L。四种重金属对刺参幼参的毒性大小依次为Cu2 >Cd2 >Zn2 >Cr6 。  相似文献   

用现今小震推断洪洞、临汾两次历史大震的震源断层   总被引：5，自引：1，他引：5  

胡新亮  刁桂苓  高景春  张四昌  啜永清  段跃荣  赵英萍  刘胜国  朱振兴  张彦清  秦清娟 《中国地震》2002,18(1):76-85

山西临汾地区是一个历史强震多发区，1303年和1695年发生了洪洞（M＝8）和临汾（M＝73／4）两次特大地震，这两次地震所在区域至今仍在持续不断的小地震活动，具有明显的大震区地震长期活动特征，我们对临汾无线传输地震台网记录的1987－1999年期间发生的1670次中，小地震重新进行了震源定位，根据对这些地震震源位置三维空间分布特征和震源机解制的分析，认为洪洞地震的震源断层应是长80km，埋深5－26km的NNE走向，高倾角的右旋走滑型断层，而临汾地震的震源断层是长70km，埋深5－22km的NWW走向，高倾角的左旋走滑型断层。这与洪洞，临汾两次大震极震区的等震线及该地区应力场的构造环境是吻合的。  相似文献   

1999年山西大同Ms 5.6地震的震源断层   总被引：9，自引：0，他引：9  

王焱  刁桂苓  张四昌  王勤彩  刘允清  朱振兴  张彦清 《中国地震》2002,18(1):96-101

大同震区先后在 1989、1991和 1999年发生MS >5地震 ,利用大同遥测地震台网的记录资料进行比较精确的地震序列震源定位 ,结合宏观烈度分布和震源机制解资料 ,详细地分析对比了 3次子序列的异同。结果显示 ,1999年MS5 .6地震的震源断层是走向NWW、长 16km、宽12km、埋深 5km以下、倾角近直立的左旋走滑断层。而前 2个子序列是NNE为主的右旋走滑断层活动所致 ,表明地震破裂方向发生了变化。这种 2个以上方向先后出现、并且强弱有别的地震破裂是普遍存在的 ,表明震源环境的复杂程度与地震序列的类型有关。虽然震区存在NE向的大王村断裂和NW向的团堡断裂 ,但目前没有证据说明震源断层和 2条构造断层连通。 3次子序列的震源断层都是走滑断层 ,也和 2条构造正断层有别。 1999年的子序列可能属于新破裂。  相似文献   

Focal Fault of the 1999 Datong Ms5.6 Earthquake in Shanxi Province   总被引：1，自引：0，他引：1  

Wang Yan  Diao Guiling  Zhang Sichang  Wang Qincai  Liu Yunqing  Zhu Zhenxing  Zhang Yanqing 《中国地震研究》2002,16(4):363-368

Several earthquakes with Ms≥5.0 occurred in the Datong seismic region in 1989,1991 and 1999,The precise focus location of the earthquake sequence was made by the records of the remote sensing seismic station network in Datong.Using that data together with macro-intensity distribution and focal mechanism solutions,we analyze the difference among three subsequences.The results show that the focal fault of the 1999 Ms 5.6 earthquake was a NWW-trending left-lateral strike-slip fault.It is 16km long and 12km wide.It developed at the depth of 5km and is nearly vertical in dip.The two previous earthquake subsequences,however,were generated by activity along NNE-trending right-lateral strike-slip fault.It can be found that the rupture directioin of the 1999 earthquake has changed.It is generally found that a rupture zone has more than two directions and has different strength along these two directions.The complicate degree of focal circumstance is related to the type of earthquake sequences.There is the NE-trending Dawangcun fault and the NW-trending Tuanbu fault in the seismic region,but no proof indicates a connection between focal faults and these two tectonic faults.The feature that focal faults of three subsequences are strike-slip is different from that of the two tectonic faults.It is suggested that the 1999 earthquake subsequence was possibly generated by a new rupture.  相似文献   

Advanced glycation endproducts in 35 types of seafood products consumed in eastern China     

WANG Jing  LI Zhenxing  PAVASE Ramesh Tushar  LIN Hong  ZOU Long  WEN Jie  LV Liangtao 《中国海洋大学学报(英文版)》2016,15(4):690-696

Advanced glycation endproducts(AGEs) have been recognized as hazards in processed foods that can induce chronic diseases such as cardiovascular disease, diabetes, and diabetic nephropathy. In this study, we investigated the AGEs contents of 35 types of industrial seafood products that are consumed frequently in eastern China. Total fluorescent AGEs level and Nε-carboxymethyl-lysine(CML) content were evaluated by fluorescence spectrophotometry and gas chromatography-mass spectrometry(GC-MS), respectively. The level of total fluorescent AGEs in seafood samples ranged from 39.37 to 1178.3 AU, and was higher in canned and packaged instant aquatic products that were processed at high temperatures. The CML content in seafood samples ranged from 44.8 to 439.1 mg per kg dried sample, and was higher in roasted seafood samples. The total fluorescent AGEs and CML content increased when seafood underwent high-temperature processing, but did not show an obvious correlation. The present study suggested that commonly consumed seafood contains different levels of AGEs, and the seafood processed at high temperatures always displays a high level of either AGEs or CML.  相似文献   

海上高产高含蜡井测试工艺优化研究和应用          下载免费PDF全文

冯大龙  黄伟  谭振兴  袁则名 《探矿工程》2021,48(6):39-43

为研究海上高产高含蜡油气井测试工艺优化方案,以东海西湖凹陷某井测试作业中所暴露出的问题为导向,开展西湖高产高含蜡储集层测试问题原因分析和国内外技术调研,同时进行了测试管柱和地面测试流程的流动性保障技术研究及原油燃烧保障技术研究。研究表明地面流程结蜡、大油嘴放喷返排出大颗粒碎屑及大产量原油燃烧导致燃烧头损坏是影响测试的主要原因,因此对测试管柱、地面测试流程及燃烧保障等进行工艺优化,以解决高产高含蜡油气井测试相关问题。  相似文献