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1.
Chia Shir Reen Chew Kit Wayne Show Pau Loke Sivakumar Manickam Ling Tau Chuan Tao Yang 《中国海洋湖沼学报》2019,37(3):898-908
Microalgae, a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical, bioenergy and food applications. This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga, Chlorella sorokiniana CY1 which was assisted with sonication. A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides, glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein). The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases, as the yield of protein increased to 77%. The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well. The optimum protein productivity was obtained with 10 s of resting time in between sonication. Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode. The optimum condition for protein extraction were found as followed: 200 g/L glucose as bottom phase with volume ratio of I:1.25, 10 s of resting time for ultrasonication, 5 s of ultrasonication in pulse mode and 0.25 g of biomass weight. The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future. 相似文献
2.
Bioremediation of Phenanthrene by Mycoplana sp. MVMB2 Isolated from Contaminated Soil 总被引：1，自引：0，他引：1
Mahalingam Brinda Lakshmi Venkataramagounder Perumal Anandaraj Manickam Velan 《洁净——土壤、空气、水》2013,41(1):86-93
The effect of nutrient and surfactant addition on the biodegradation of phenanthrene was studied in a batch scale soil–slurry system using isolated Mycoplana sp. MVMB2strain. The study was conducted using an artificially phenanthrene spiked and as well as contaminated soil from petrochemical industrial site. Maximum phenanthrene degradation and subsequent high microbial growth were observed at optimum pH (pH 6) and C/N/P ratio (100:20:3). To investigate maximum substrate degradation potential of Mycoplana sp. MVMB2, very high concentrations of phenanthrene (50–200 mg/kg soil) were used. The organism was capable of degrading >60% for a concentration below 20 mg/kg soil and >40% for concentrations up to 200 mg/kg within 8 days. Further the influence of five different surfactants namely Span 80, Tween 20, Triton X‐100, cetyl trimethyl ammonium bromide, and sodium dodecyl sulfate were tested at their critical micelle concentration (CMC) levels for phenanthrene degradation in the soil. The addition of surfactant enhanced the biodegradation and a maximum of 84.49% was obtained for Triton X‐100. Complete phenanthrene degradation by Mycoplana sp. MVMB2 was observed at 3 CMC concentration of Triton X‐100. The optimized parameters obtained were used for the degradation of phenanthrene present in the contaminated soil and 98.6% biodegradation was obtained. Thus, the results obtained in the study suggested that biodegradation of phenanthrene by Mycoplana sp. MVMB2 appeared to be feasible to remediate phenanthrene rich contaminated sites. 相似文献
3.
Surendar Kumar 《Tectonophysics》1975,27(2):167-176
The occurrence of earthquakes all along the West Coast of India and in the Arabian Sea is explained systematically in terms of the interaction between the underthrusting Indian ocean floor and the Indian subcontinent. This north ward compression due to the repeated underthrusting of the oceanic floor has caused the formation of trenches, grabens and normal faults on the continental margin. 相似文献
4.
An attempt has been made to produce the geological map of Ladakh, Lahaul and Spiti regions of Himalaya on the basis of the field work done by the authors and the observations made by earlier workers. The stratigraphy of this region has been discussed in the light of new fossil finds from different stratigraphical horizons. Stratigraphical columns have been prepared and the formations of this region have been correlated with those exposed in other parts of the Himalaya. The age of the flysch deposits (Indus Flysch of Ladakh and Chikkim Shales of Spiti) has been defined on the basis of the microfauna.
Zusammenfassung Aus Feldarbeiten und bereits vorliegenden Fakten resultierte eine neue geologische Karte des Gebietes von Ladakh, Lahaul und Spiti im Himalaya. Die Stratigraphie wird mit Hilfe neuer Fossilfunde geändert und mit anderen Teilgebieten des Himalayas verglichen. Das Alter der Flyschserien (Indus-Flysch von Ladakh und Chikkim-Shales von Spiti) wurde mit Hilfe von Mikrofaunen bestimmt.
Résumé Les levés de terrain des auteurs, combinés avec des travaux antérieurs, ont permis de dresser une nouvelle carte géologique du Ladakh, Lahaul et Spiti dans l'Himalaya. La stratigraphie a été modifiée grâce à de nouvelles découvertes de fossiles et comparée avec celle d'autres régions de l'Himalaya. L'âge de la série du flysch (Flysch de l'indus de Ladakh et schistes de Chikkim de Spiti) a été déterminé à l'aide de microfaunes.
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5.
Anuj Nandi Sivakumar G. Manickam A. R. Rao Sandip K. Chakrabarti 《Monthly notices of the Royal Astronomical Society》2001,324(1):267-272
A few classes of the light curve of the black hole candidate GRS 1915+105 have been analysed in detail. We discover that unlike the previous findings, quasi-periodic oscillations (QPOs) occasionally occur even in the so-called 'On' or softer states. Such findings may require a revision of the accretion/wind scenario of the black hole candidates. We conjecture that considerable winds that are produced in 'Off' states cool down as a result of Comptonization and fall back to the disc, creating an excess accretion rate and producing the so-called 'On' state. After the drainage of the excess matter, the disc goes back to the 'Off' state. Our findings strengthen the shock oscillation model for QPOs. 相似文献
6.
This study presents the degradation of phenanthrene by Mycoplana sp. MVMB2 isolated from petroleum contaminated soil and the media optimization by factorial design experiments. The Plackett–Burman design was used to evaluate the effects of eight variables (potassium dihydrogen phosphate, disodium hydrogen phosphate, magnesium sulfate, calcium chloride, ferrous sulfate, glucose, inoculum concentration, and phenanthrene concentration) on phenanthrene degradation. Based on the results, the critical medium components having significant influence on the degradation were found to be disodium hydrogen phosphate, magnesium sulfate, ferrous sulfate, and phenanthrene. Furthermore, these four variables were used as central composite design parameters. The optimum minimal salt medium composition obtained by conventional and factorial design experiments for the degradation of phenanthrene by Mycoplana sp. MVMB2 at pH 6.5 and 30°C were found to be, potassium 2.5 g/L dihydrogen phosphate, 0.3505 g/L disodium hydrogen phosphate, 0.5501 g/L magnesium sulfate, 0.02 g/L calcium chloride, 0.0261 g/L ferrous sulfate, 0.6756 g/L phenanthrene, 0.5 g/L glucose, 0.5 g/L ammonium sulfate, and inoculum 5% v/v. The phenanthrene degradation was confirmed by analyzing the metabolites formed. 相似文献
7.
Sahadevan Renganathan Jayabalan Kalpana Mahendradas Dharmendira Kumar Manickam Velan 《洁净——土壤、空气、水》2009,37(11):901-907
Nymphaea rubra stem was used as a low cost and easily available biosorbent for the removal of Reactive Red 2 dye from an aqueous solution. Initially, the effects of biosorbent dosage (0.2–1.0 g L–1), pH (1–6), and dye concentration (30–110 mg L–1) on dye removal were studied. Batch experiments were carried out for biosorption kinetics and isotherm studies. The results showed that dye uptake capacity was found to increase with a decrease in biosorbent dosage. Equilibrium uptake capacity was found to be greatest at a pH value of 2.0, when compared to all other pH values studied. The equilibrium biosorption isotherms were analyzed by the Freundlich and Langmuir models. The equilibrium data was found to fit very well with the Freundlich isotherm model when compared to the Langmuir isotherm model. The kinetic data was analyzed using pseudo-first order and pseudo-second order kinetic models. From the results, it was observed that the kinetic data was found to fit the pseudo-second order kinetic model very well. The surface morphology of the stem of the N. rubra biosorbent was exemplified by scanning electron microscopy. Fourier transform infrared analysis was employed to confirm the existence of an amine group in the stem of N. rubra. 相似文献
8.
The epicentre of the destructive 20 October 1991 earthquake is in the north-east of the Uttarkashi region of the Higher Himalaya. The earthquake was felt up to 250–350 km away from Poh and Keylong in the north to Delhi in the south and beyond Chandigarh in the west. Seismologists of the Seismotectonic Group of Wadia Institute of Himalayan Geology studied fissures, surface breaks, and the foreshock and aftershock activity caused by this event. Land fissures show normal dislocations of 0.06–1 m, run E-W and NE-SW in the epicentral region and could be followed for 30–40 km. 相似文献
9.
This study presents the degradation of phenanthrene using immobilized Mycoplana sp. MVMB2 isolated from contaminated soil. Papaya stem pretreated by two stage processes, treating with acid or alkali and drying, was used for the immobilization of Mycoplana sp. Alkali pretreated papaya stem was found to be most effective in cell uptake compared to acid treated one. The maximum immobilization capacity at various physiochemical conditions for the alkali pretreated papaya stem was found to be at 320 min time, pH 6.5, 30°C temperature, and 18.6 × 106 cells/mL initial concentrations. The adsorption mechanism of Mycoplana sp. MVMB2 on pretreated papaya stem was assessed using various kinetic and isotherm models. The immobilization of Mycoplana sp. MVMB2 on to pretreated papaya stem was corroborated by scanning electron microscopy and Fourier transformed IR spectroscopy analysis. The performance of immobilized cells in batch reactor showed more than 95% phenanthrene degradation within 72 h, whereas, free cells were found to require 120 h. The immobilized cells also showed better degradation performance in the packed column study. 相似文献
10.
Comparison of “argon” absolute age determinations of crystalline rocks of the “Crystalline Nappe” (Kumar, 1971, unpublished Ph. D. thesis) and of the “root-zone” (Jaspa granite) has led to an important conclusion concerning the interpretation of the development of the “crystalline nappe” and its rejuvenation. 相似文献