The effects of electrochemical oxidation on in-vivo fluorescence and toxin content in Microcystis aeruginosa culture     

ROZINA Tinkara  ELER?EK Tina  Maja ZUPAN?I? JUSTIN  MEGLI? Andrej  LE?TAN Domen  SEDMAK Bojan 《中国海洋湖沼学报》2018,(4)

The increasing occurrence of cyanobacterial blooms in water bodies is a serious threat to the environment. Efficient in-lake treatment methods for the control of cyanobacteria proliferation are needed, their in-vivo detection to obtain a real-time response to their presence, as well as the information about their physiological state after the applied treatment. In-vivo fluorescence measurements of photosynthetic pigments have proved to be effective for quantitative and qualitative detection of phytoplankton in a water environment. In the experiment, chlorophyll and phycocyanin fluorescence sensors were used concurrently to detect stress caused by electrochemical oxidation applying an electrolytic cell equipped with borondoped diamond electrodes on a laboratory culture of cyanobacteria Microcystis aeruginosa PCC 7806. The inflicted injuries were reflected in a clear transient increase in the phycocyanin fluorescence signal(for 104 %? 43%) 24 h after the treatment, which was not the case for the chlorophyll fluorescence signal. In the next 72 h of observation, the fluorescence signals decreased(on 40% of the starting signal) indicating a reduction of cell number, which was confirmed by cell count(24% reduction of the starting concentration) and analysis of extracted chlorophyll and phycocyanin pigment. These results demonstrate the viability of the combined application of two sensors as a useful tool for in-vivo detection of induced stress, providing real-time information needed for the evaluation of the efficiency of the in-lake treatment and decision upon the necessity of its repetition. The electrochemical treatment also resulted in a lower free microcystins concentration compared to control.  相似文献   

11颗类星体光谱(英文)     

陈建生  P.A.Shaver  I.J.Danziger 《中国天文和天体物理学报》1985,(4)

对天区0112-35的UKST物端棱镜底片上的11个类星体候选者进行了光谱观测,证实为类星体,给出了它们的红移值、证认图及光谱图。11个类星体中有6个属于角距小于4角分的3对类星体;1个类星体-星系对。  相似文献   

农田灌溉对亚洲西部地区环境的影响     

I.纳哈  孙仁华 《地理科学进展》1985,4(2):15-19

引言农田灌溉是一种最易提高干旱地区农业产量的方法。灌溉农业与旱作农业相比,谷物产量可提高6倍,根块植物产量可提高4—5倍。因此,农田灌溉在渴望得到更多粮食的干旱地区,可以发挥巨大的作用。农田灌溉使这些地区获得高产稳产,同时减轻了干旱和沙漠化的威胁。  相似文献   

深海中的河流地貌：对拉波拉多海西北大西洋洋中河道冰成海底水系的新…     

Klauc.  I 龚建明 《海洋地质前沿》1997,(1):10-12

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Observation of clouds and solar radiation over the Pacific Ocean as relation to global climate     

Farn Parungo  Clarence Nagamoto  Cecilia M. I. R. Girz  Jeff Torgerson  Zhou Mingyu 《海洋学报(英文版)》1995,14(2):201-207

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The influence of salinity on the heat-shock protein response of Potamocorbula amurensis (Bivalvia)   总被引：1，自引：0，他引：1  

Werner I 《Marine environmental research》2004,58(2-5):803-807

For biomarkers to be useful in assessing anthropogenic impacts in field studies involving aquatic organisms, they should not be affected by naturally occurring changes in environmental parameters such as salinity. This is especially important in estuarine environments and for relatively unspecific biomarkers like heat-shock proteins (hsps, stress proteins). In this study, the heat-shock protein response was measured in the euryhaline clam, Potamocorbula amurensis, after exposure to a range of salinities reflecting normal and extreme environmental conditions in Northern San Francisco Bay, California. The ability to raise cellular hsp70 levels in response to heat-shock was significantly impaired in P. amurensis collected from a low (0.5 ppt) salinity field site, and after 14 day exposure to low salinity in the laboratory.  相似文献   

Fatal attraction: synthetic musk fragrances compromise multixenobiotic defense systems in mussels     

Luckenbach T  Corsi I  Epel D 《Marine environmental research》2004,58(2-5):215-219

We studied interactions of nitromusk compounds musk ketone and musk xylene and polycyclic musks Galaxolide trade mark (HHCB), Celestolide trade mark (ADBI), Tetralide trade mark (AHTN), and Traseolide trade mark (AITI) with multixenobiotic resistance (mxr) transporters in gill tissue of the marine mussel Mytilus californianus (Conrad, 1837). A competitive substrate transport test with rhodamine B was used to assay modulation of transport activity by musks. All tested musks inhibited the transport activity in the low microm range as indicated by increased accumulation of rhodamine B in the tissue. Compared to known substrates of mxr transporters, the effective concentration range was similar to quinidine and about 100 times higher than verapamil. Musk ketone and musk xylene also inhibited efflux of rhodamine B from gill tissue which was loaded with the dye and subsequently incubated with these compounds. Synthetic musk compounds are persistent environmental pollutants in aquatic environments with a high potential to bioaccumulate. As potent inhibitors of mxr transporters they may also play a role as chemosensitizers that enable toxic mxr substrates to accumulate in cells of aquatic organisms.  相似文献   

Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides)     

Blum JL  Knoebl I  Larkin P  Kroll KJ  Denslow ND 《Marine environmental research》2004,58(2-5):565-569

In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N(1)-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted.  相似文献   

Oxidative stress in ecotoxicology: from the analysis of individual antioxidants to a more integrated approach     

Regoli F  Gorbi S  Frenzilli G  Nigro M  Corsi I  Focardi S  Winston GW 《Marine environmental research》2002,54(3-5):419-423

An integrate approach will be discussed for investigations on oxidative stress in xenobiotic toxicity. While the analysis of individual antioxidants is useful for their sensitivity and to understand the mode of action of a stressor, the integration with the analysis of the total antioxidant capacity provides a more holistic assessment of the overall biological significance of such variations. TOSC has a greater predictive value on the health condition of the organisms and allows to discriminate the different role of specific ROS in oxidative stress syndrome.  相似文献   

东北和西南日本岩浆气N2／Ar比值的差异：板块俯冲不同状态的新证据     

Kita  I 郭彤楼 《海洋地质译丛》1994,(4):19-22

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