利用扩增vvhA基因的环介导等温扩增技术快速，特异而敏感地检测创伤弧菌     

张丽娜  王明义  丛殿霞  丁淑研  丛日楠  岳进勇  耿建利  胡成进 《海洋学报(英文版)》2018,37(4):83-88

创伤弧菌是一种可感染人类的河口病原菌。建立快速，特异而敏感的检测方法，有助于创伤弧菌感染的早期疾病诊断和及时治疗。本研究设计了针对vvhA基因的一系列引物（包括两对外部引物和两对内部引物），采用环介导等温扩增技术（LAMP）来检测创伤弧菌。结果显示，本方法的最适扩增温度是63℃，反应仅需35分钟。扩增产物不仅可以用含有DNA ladder的琼脂糖凝胶电泳检出，也可借助钙黄绿素直接肉眼观察。采用45株菌株检测该方法的特异性，其中所有创伤弧菌均被检出而其他菌株检测结果皆为阴性。本方法的敏感性是普通PCR扩增的100倍，同时利用该方法可以准确检测出所有的模拟样品、临床标本及环境样品。与其他已知方法比较，针对vvhA基因的介导等温扩增技术可以快速、简单、敏感及特异地鉴定创伤弧菌。  相似文献   

中国土地利用空间格局刻画方法研究   总被引：7，自引：1，他引：6  

鞠洪润  左丽君  张增祥  赵晓丽  汪潇  温庆可  刘芳  徐进勇  刘斌  易玲  胡顺光  孙菲菲  汤占中 《地理学报》2020,75(1):143-159

土地利用空间格局研究是土地利用/覆盖变化（LUCC）理论和实践的基础,对土地利用空间格局进行有效刻画有利于国土资源空间优化,提升土地利用规划和管理水平。由于土地利用空间格局的研究范畴尚不明晰,目前土地利用空间格局的研究对形状、斑块分布和结构等方面关注较少,缺乏对不同土地利用类型间相互关系的研究;同时,格局指标繁多且存在较大的相关性,如何建立指标与土地利用空间格局的对应关系值得进一步研究。本文在深刻理解土地利用空间格局内涵的基础上,将面积、形状和斑块分布总结为土地利用几何特征,将结构和多样性总结为土地利用类型特征,建立了土地利用空间格局刻画指标体系,利用模糊C均值聚类分析,明确了指标与空间格局间的对应关系。结果表明,中国土地利用几何特征可以划分为简单大斑块型、复杂大斑块型、复杂小斑块型、简单小斑块型和散布型五种,不同的几何特征反映了不同土地利用类型的面积、形状和斑块分布的特点,体现了区域土地利用类型的组合关系。2010年中国土地利用共存在61种不同的结构,但主要的结构类型仅有14种,结构特征具有明显的空间聚集性,体现了不同土地利用类型的空间分异性。中国土地利用多样性以3~5类为主,占比达66.69%,其特征总体上呈现“东北、东南高,西北低”的态势。该研究丰富了土地利用空间格局研究的理论体系,填补了中国土地利用整体空间格局刻画的空白。  相似文献   

纳米比亚罗辛地区白岗岩型铀矿成因研究     

韩军  陈金勇  范洪海  王生云  陈东欢  修群业 《地质学报》2021,95(4):1019-1028

纳米比亚罗辛地区白岗岩型铀矿床产于白岗岩穹窿边部白岗岩与达玛拉基底变质岩内外接触带,以及变质岩片理、褶皱、转折等部位.已有矿床晶质铀矿年龄介于494～508Ma之间,既是D、E两期白岗岩重熔年龄,也是近水平方向韧性剪切变形活动时代.白岗岩型铀矿床铀矿物以晶质铀矿、钍铀矿、钛铀矿为主,晶质铀矿及伴生的锆石、独居石等矿物具有全自形或半自形晶粒状结构、浸染状构造,具有岩浆分异结晶特征.铀铅同位素示踪分析表明铀来自达玛拉基底岩系和A、B、F期白岗岩.含矿的D、E白岗岩是基底变质岩和早期白岗岩部分重熔形成的.以晶质铀矿为主的白岗岩型铀矿床是在达玛拉造山期岩浆形成演化阶段,由结晶分异作用形成铀预富集基础上,在造山期后韧性变形作用下白岗岩再次重熔富集成矿的.  相似文献   

Effect of wool fiber addition on the reinforcement of loose sands by microbially induced carbonate precipitation (MICP): mechanical property and underlying mechanism     

Yao  Dunfan  Wu  Jiao  Wang  Guowei  Wang  Pengbo  Zheng  Jun-Jie  Yan  Jinyong  Xu  Li  Yan  Yunjun 《Acta Geotechnica》2021,16(5):1401-1416

Acta Geotechnica - Animal fibers with α-keratin had obvious advantages of mechanical strength and durability on reinforced microbially induced carbonate precipitation (MICP)-cemented loose...  相似文献   

Rapid,specific and sensitive detection of Vibrio vulnificus by loop-mediated isothermal amplification targeted to vvhA gene     

ZHANG Lina  WANG Mingyi  CONG Dianxia  DING Shuyan  CONG Rinan  YUE Jinyong  GENG Jianli  HU Chengjin 《海洋学报(英文版)》2018,(4)

Vibrio vulnificus is an estuarine bacterial pathogen for human.The rapid,specific and sensitive detection of V.vulnificus is urgently needed for early disease diagnosis and timely treatment of V.vulnificus infection.In the study,a loop-mediated isothermal amplification(LAMP) technique was developed for V.vulnificus detection with a set of primers,composed of two out primers and two inner primers targeted to vvh A gene.The optimal amplification temperature was 63°C and the reaction only took 35 min.The amplification products could not only be detected by agarose gel electrophoresis with ladder-like pattern bands,but also could be visualized using calcein with naked eye directly.Forty-five strains were tested for the specificity of LAMP assay,and all the V.vulnificus strains were identified correctly while other strains were negative results.The sensitive of the new LAMP assay was 100-fold more sensitive than the conventional PCR.Meanwhile,all the V.vulnificus strains were detected correctly in spiked,clinical and environmental samples by the new LAMP assay.Compared with other well-known techniques,the new LAMP assay targeted to vvh A gene was extremely rapid,simple,sensitive and specific for V.vulnificus identification.  相似文献   

日本海西南陆坡全新世对马暖流演化的沉积学和地球化学记录     

徐兆凯  崔镇勇  林东日  李铁刚  李安春 《海洋地质与第四纪地质》2010,(1)

对日本海西南陆坡对马暖流主流轴下方高沉积速率沉积物柱状样,进行了沉积学和地球化学系统研究,以探讨全新世以来对马暖流在研究区的形成与演化,并得出了与附近地区古海洋学方法非常相近的结果,从而为今后研究区的对马暖流演化研究提供了沉积学和地球化学指标。据此,该柱状样中所记录的对马暖流演化可划分为5个主要阶段。全新世初期(9.6kaBP),对马暖流开始进入日本海,其强度较弱,同时,伴随着富营养东海沿岸水的影响,海底环境由还原性向氧化性转变;6.5kaBP时,对马暖流控制下的日本海南部现代对流模式最终建立起来,东海沿岸水的影响基本消失,随后,对马暖流明显减弱;3.0kaBP时,对马暖流再次增强并基本达到现在水平,且在随后的3ka里保持总体稳定,期间在2.0～0.7kaBP存在一次较弱的减弱过程。  相似文献   

2022年春季海洋天气评述          下载免费PDF全文

张增海  刘涛  尹尽勇  郭乙莹  张浩 《山东气象》2022,42(3):109-122

2022年春季(3—5月)北半球极涡呈单极型分布,形状狭长,极涡强度与历史同期相当。北半球中高纬度西风带呈4波型分布。3月,我国北方的大部分地区及北部海域受西北气流控制;4月,东亚大槽加深,高压脊区较历史同期偏强;5月,中高纬环流调整为“两槽两脊”型。我国近海出现12次大风过程,其中冷空气大风过程4次,冷空气和温带气旋共同影响的大风过程3次,温带气旋大风过程4次,冷空气与热带气旋共同影响的大风过程1次。近海共出现10次比较明显的海雾过程,其中3月4次,4月3次,5月3次。西北太平洋和南海有2个热带气旋生成,接近常年同期平均值;全球其他海域有12个热带气旋生成,较历史同期平均值偏少5.7个。近海浪高2.0 m以上的海浪过程有12次,总日数为44 d。春季各月我国近海海面温度整体呈上升趋势,北方海域升温幅度大于南方海域。  相似文献   

2020年秋季海洋天气评述          下载免费PDF全文

孙舒悦  尹尽勇  张增海  柳龙生 《山东气象》2021,41(1):19-29

2020年秋季(9—11月)大气环流特征表现为,北半球极涡呈单极型分布,中高纬环流呈4波型。9—11月,欧亚大陆中高纬环流经向度不断加大,冷空气势力增强。西太平洋副热带高压较历史平均偏强,热带气旋活动频繁。我国近海出现了19次8级以上大风过程,其中冷空气大风过程6次,台风大风过程4次,入海气旋大风过程1次,冷空气与热带气旋共同影响的大风过程7次,冷空气和温带气旋共同影响的大风过程1次。西北太平洋和南海共生成13个热带气旋,其中10月共有7个热带气旋生成,追平10月热带气旋生成数的历史最高纪录;全球其他海域共生成热带气旋26个。我国近海未出现2 m以上大浪过程的天数仅有12 d,约占秋季总日数的13％。秋季,我国近海海域呈明显降温过程,北部海域的降温幅度明显大于南部海域,受连续北上影响我国北部海域的热带气旋活动影响,9月黄海东部及东海东部的海面温度较气候态明显偏低。  相似文献   

Rare earth elements in bottom sediments of major rivers around the Yellow Sea: implications for sediment provenance     

Zhaokai Xu  Dhongil Lim  Jinyong Choi  Shouye Yang  Hoisoo Jung 《Geo-Marine Letters》2009,29(5):291-300

Rare earth elements (REEs) of 91 fine-grained bottom sediment samples from five major rivers in Korea (the Han, Keum, and Yeongsan) and China (the Changjiang and Huanghe) were studied to investigate their potential as source indicator for Yellow Sea shelf sediments, this being the first synthetic report on REE trends for bottom sediments of these rivers. The results show distinct differences in REE contents and their upper continental crust (UCC)-normalized patterns: compared to heavy rare earth elements (HREEs), light rare earth elements (LREEs) are highly enriched in Korean river sediments, in contrast to Chinese river sediments that have a characteristic positive Eu anomaly. This phenomenon is observed also in primary source rocks within the river catchments. This suggests that source rock composition is the primary control on the REE signatures of these river sediments, due largely to variations in the levels of chlorite and monazite, which are more abundant in Korean bottom river sediments. Systematic variations in ΣLREE/ΣHREE ratios, and in (La/Yb)–(Gd/Yb)_UCC but also (La/Lu)–(La/Y)_UCC and (La/Y)–(Gd/Lu)_UCC relations have the greatest discriminatory power. These findings are consistent with, but considerably expand on the limited datasets available to date for suspended sediments. Evidently, the REE fingerprints of these river sediments can serve as a useful diagnostic tool for tracing the provenance of sediments in the Yellow Sea, and for reconstructing their dispersal patterns and the circulation system of the modern shelf, as well as the paleoenvironmental record of this and adjoining marginal seas.  相似文献   

Spatial and temporal variations of coastlines in northern China (2000-2012)     

XU Jinyong  ZHANG Zengxiang  ZHAO Xiaoli  WEN Qingke  ZUO Lijun  WANG Xiao  YI Ling 《地理学报》2014,24(1):18-32

This study examined the spatial distribution of the continent coastline in northern China using remote sensing and GIS techniques, and calculated the fractal dimension of the coastline by box-counting method, with a time span from 2000 to 2012. Moreover, we analyzed the characteristics of spatial-temporal changes in the coastline's length and fractal dimension, the relationship between the length change and fractal dimension change, and the driving forces of coastline changes in northern China. During the research period, the coastline of the study area increased by 637.95 km, at a rate of 53.16 km per year. On the regional level, the most significant change in coastline length was observed in Tianjin and Hebei. Temporally, the northern China coastline grew faster after 2008. The most dramatic growth was found between 2010 and 2011, with an increasing rate of 2.49% per year. The fractal dimension of the coastline in northern China was increasing during the research period, and the most dramatic increase occurred in Bohai Rim. There is a strong-positive linear relationship between the historical coastline length and fractal dimension (the correlation coefficient was 0.9962). Through statistical analysis of a large number of local coastline changes, it can be found that the increase (or decrease) of local coastline length will, in most cases, lead to the increase (or decrease) of the whole coastline fractal dimension. Civil-coastal engineering construction was the most important factor driving the coastline change in northern China. Port construction, fisheries facilities and salt factories were the top three construction activities. Compared to human activities, the influence of natural processes such as estuarine deposit and erosion were relatively small.  相似文献