Evaluating the effects of catch-and-release angling on Cape stumpnose Rhabdosargus holubi in a South African estuary     

NK Arkert  A-R Childs  MC Parkinson  AC Winkler  E Butler  S Mannheim 《African Journal of Marine Science》2018,40(3):235-244

Fisheries managers are increasingly promoting catch-and-release (C&R) to manage recreationally angled fish stocks. Despite this, there is a scarcity of information on the effects of C&R on estuarine-dependent species. Cape stumpnose Rhabdosargus holubi dominates the recreational fisheries catch and provides an important source of food for subsistence fishers in some temperate South African estuaries. The health and survival of R. holubi exposed to a C&R event was investigated by examining their physiological stress response (blood glucose and lactate), reflex impairment (reflex action mortality predictors [RAMP]) and short-term (12-hour) survival. Fish were captured and exposed to one of three air-exposure treatments: 0 s, 30 s or 90 s. Stress and health were measured either immediately (immediate) or one hour after (delayed) the C&R event. There was no significant difference in blood glucose between air-exposure treatments, but there was a significant difference between the mean immediate and delayed glucose levels within each treatment (F_(2,143) = 81.8, p < 0.01). In contrast, blood lactate level was significantly higher in the 90-s treatment (p < 0.05). Immediate blood lactate levels were significantly lower than the delayed samples for each treatment (F = 4.29, p = 0.02; n = 169). Although all fish exhibited at least one reflex impairment, the RAMP score was significantly higher in the 90-s air-exposure treatment (H_(2,86) = 9.73, p = 0.007). Also, RAMP scores were significantly lower in the delayed samples (p < 0.01). Although short-term mortality was relatively low (2.3%) for this species, it was highest in the 90-s treatment (7%). These results suggest that physiological stress is higher when R. holubi are exposed to longer periods of air exposure and that the physiological stress of fish subject to a C&R event is best measured after a delay.  相似文献   

FeS-Induced Radical Formation and Its Effect on Plasmid DNA     

D. Rickard  B. Hatton  D. M. Murphy  I. B. Butler  A. Oldroyd  A. Hann 《Aquatic Geochemistry》2011,17(4-5):545-566

Plasmid DNA was incubated at 25°C with aqueous solutions of dissolved Fe(II), S(-II), and nanoparticulate FeS with a mackinawite structure, FeS_m. At ??0.1 mM total dissolved Fe(II) and S(-II), an increase in the proportion of the relaxed plasmid DNA occurs, through scission of the DNA backbone. In solutions where FeS_m was precipitated, nanoparticulate FeS_m binds to the DNA molecules. In solutions with concentrations below the FeS_m solubility product, nicking of supercoiled pDNA occurs. Plasmid DNA appears to be a sensitive proxy for radical reactions. The reactant is proposed to be a sulfur-based radical produced from the iron-catalyzed decomposition of bisulfide, in a manner analogous to the Fenton reaction. This is further supported by experiments that suggest that sulfide free radicals are produced during the photolysis of aqueous solutions of polysulfides. Supercoiling of DNA affects nearly all DNA?Cprotein transactions so the observation of relaxation of supercoiled forms through reaction with FeS solutions has direct implications to biochemistry. The results of this experimentation suggest that genotoxicity in FeS-rich systems is a further contributory factor to the limited survival of organisms in sulfidic environments. Mutations resulting from the interactions of organisms and mobile elements, such as plasmids, in sediments will also be affected in sulfide-rich environments.  相似文献   

The Shapes of Splash-Form Tektites: Their Geometrical Analysis, Classification and Mechanics of Formation     

Mel R. Stauffer  Samuel L. Butler 《Earth, Moon, and Planets》2010,107(2-4):169-196

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Evaporative weathering of petroleum residues: The age of pelagic tar     

James N. Butler 《Marine Chemistry》1975,3(1):9-21

The rate of evaporative weathering of a component of crude oil residue is assumed to be proportional to its equilibrium vapor pressure and to the amount remaining in the sample. This model is tested using literature data on normal paraffin profiles of crude oil weathered artificially and gas chromatographic data on crude oil residues weathered on rocky shores. Semiquantitative agreement over the time range from 1 to 400 days is demonstrated. Application of the model to gas chromatograms of pelagic tar lumps collected at Station S near Bermuda in 1972 implies that most of these lumps were formed by fragmentation of much larger and older masses, some within less than one day of the time they were collected. The weathering of pelagic tar thus involves a great deal more physical fragmentation than was previously supposed.  相似文献   

Voltammetric estimation of iron(III) thermodynamic stability constants for catecholate siderophores isolated from marine bacteria and cyanobacteria     

Brent L. Lewis  Pamela D. Holt  Steven W. Taylor  Steven W. Wilhelm  Charles G. Trick  Alison Butler  George W. Luther  III 《Marine Chemistry》1995,50(1-4)

Thermodynamic stability constants have been estimated for the complexation of iron(III) with catecholate-type siderophores isolated from the marine bacterium Alteromonas luteoviolacea and from the marine cyanobacterium Synechococcus sp. PCC 7002. Stability constants were determined utilizing the “chelate scale” of Taylor et al. (1994). The scale is based upon a linear relationship between the reduction potentials and the pH-independent thermodynamic stability constants for known iron(III) complexes. Log K values for the alterobactin B ferric iron complex are 43.6 ± 1.5 at pH 8.2 and 37.6 ± 1.2 at pH 6, consistent with a shift from bis-catecholate to monosalicylate/monocatecholate iron coordination with decreasing pH. Synechococcus isolates PCC 7002 Nos. 1 and 3 formed iron(III) complexes with stability constants of approximately 38.1 ± 1.2 and 42.3 ± 1.5, respectively. The binding strengths of the iron(III) complexes examined in this study are quite high, suggesting that catecholate siderophores may play a role in the solubilization and biological uptake of iron in the marine environment.  相似文献   

Coulometric total carbon dioxide analysis for marine studies: maximizing the performance of an automated gas extraction system and coulometric detector     

K.M. Johnson  K.D. Wills  D.B. Butler  W.K. Johnson  C.S. Wong 《Marine Chemistry》1993,44(2-4)

A global survey of the distribution of dissolved CO₂ taking advantage of sampling opportunities provided by the World Ocean Circulation Experiment: World Hydrographic Program (WOCE-WHP) is being carried out through 1995. Goals include the measurement of oceanic inorganic carbon transport and the development of a data base from which future fossil-fuel CO₂ build-up can be monitored. The analytical method selected for total carbon dioxide (C_T) is gas extraction of acidified seawater with coulometric titration of the acid formed by the resultant carbon dioxide and monoethanolamine. To combine high accuracy and precision (± 1.5 μmol/kg for C_T ≥ 2000 μmol/kg) with a high rate of analysis, we have modified an automated single-parameter system. Following prototype development between 1987 and 1990, an instrument emerged with the acronym Somma standing for single-operator multiparameter metabolic analyzer. Improved functional and operating procedures have integrated electronic calibration, CO₂ gas calibration, and sample analysis with automated pressure, temperature, and conductivity (salinity) sensing into a single convenient transportable package.  相似文献   

Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure   总被引：3，自引：0，他引：3  

Hayes RA  Regondi S  Winter MJ  Butler PJ  Agradi E  Taylor EW  Kevin Chipman J 《Marine environmental research》2004,58(2-5):665-669

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.  相似文献   

Voracious planktonic hydroids: unexpected predatory impact on a coastal marine ecosystem     

Laurence P. Madin  Stephen M. Bollens  Erich Horgan  Mari Butler  Jeffrey Runge  Barbara K. Sullivan  Grace Klein-Macphee  Edward Durbin  Ann G. Durbin  Donna Van Keuren  Stphane Plourde  Ann Bucklin  M. Elizabeth Clarke 《Deep Sea Research Part II: Topical Studies in Oceanography》1996,43(7-8)

Hydroids are typically attached, benthic cnidarians that feed on a variety of small prey. During sampling on Georges Bank in spring 1994, we found huge numbers of hydroids suspended in the plankton. They fed on young stages of copepods that are an important prey for fish, as well as on young fish themselves. Two independent methods were used to estimate feeding rates of the hydroids; both indicate that the hydroids are capable of consuming from 50% to over 100% of the daily production of young copepods. These results suggest that hydroids can have a profound effect on the population dynamics of zooplankton and young fish on Georges Bank.  相似文献   

Processes affecting transfer of sediment and colloids,with associated phosphorus,from intensively farmed grasslands: tracing sediment and organic matter     

S. J. Granger  R. Bol  P. J. Butler  P. M. Haygarth  P. Naden  G. Old  P. N. Owens  B. P. G. Smith 《水文研究》2007,21(3):417-422

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Seasonal change in the deep atmosphere of Uranus     

Mark D Hofstadter  Bryan J Butler 《Icarus》2003,165(1):168-180

We present high-resolution radio maps of Uranus, made from data collected in 1994 at wavelengths of 2 and 6 cm, which show large-scale changes occurring deep and rapidly in the troposphere. Brightness features in these maps are significantly different from those observed throughout the 1980's. These differences are not due to the changing viewing geometry, but result from atmospheric changes in the 5 to 50 bar region. All the observations show strong latitudinal variations in absorber abundance and/or temperature, causing the South Pole to appear brighter than lower latitudes. The transition between bright pole and darker latitudes is always near −45°, but between 1989 and 1994 the contrast between the regions increased significantly. This suggests that the large-scale circulation in the upper 50 bars of the uranian Southern Hemisphere changed. Older, disk-averaged microwave observations have suggested that seasonal variability occurs, but these new maps are the first to provide detailed timing and location information which can be used to test dynamical models.  相似文献