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In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N(1)-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted. 相似文献
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The identification, remedial treatment, and monitoring of contaminated sediments are among the priorities for managers of the Tampa Bay Estuary. Tampa Bay, as an urbanized estuary, is subject to the input of watershed sources of chemical contaminants, including metals, pesticides, and organic chemicals. Although the use of biological indicators and their incorporation into multi-metric indices is not new, the refinement and applications of such techniques for determining environmental condition still require further development and exploration. We present a single Tampa Bay Benthic Index (TBBI) that was developed specifically for Tampa Bay. Stepwise discriminant analysis was applied to a comprehensive list of potential benthic metrics. Results from the stepwise procedure identified the metrics that best discriminated between "healthy" and "degraded" conditions, as defined by sediment contaminant effect levels and dissolved oxygen. Discriminant analysis was then applied to the resultant three variables to determine the linear combination for the index. 相似文献
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为从根本上解决常规钢衬钢筋混凝土压力管道因运行期的裂缝过宽而带来的结构耐久性问题,采取将常规混凝土改性为高性能的钢纤维混凝土或钢纤维自应力混凝土的方法。以某水电站全背坝面管为原型,以1：10缩尺制作了钢衬钢筋钢纤维混凝土压力管道模型和钢衬钢筋钢纤维自应力混凝土压力管道模型。试验结果表明,改性为钢纤维混凝土的压力管道表现出很好的限裂能力,其初裂荷载有一定的提高,管道裂缝宽度显著下降;改性为钢纤维自应力混凝土的压力管道表现出很好的抗裂能力,管道的初裂荷载有了大幅度的提高,钢材的性能也得到了较充分的利用。模型试验的结果显示了改性钢衬钢筋混凝土压力管道良好的应用前景。 相似文献
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Permeameter data verify new turbulence process for MODFLOW 总被引：3，自引：0，他引：3
A sample of Key Largo Limestone from southern Florida exhibited turbulent flow behavior along three orthogonal axes as reported in recently published permeameter experiments. The limestone sample was a cube measuring 0.2 m on edge. The published nonlinear relation between hydraulic gradient and discharge was simulated using the turbulent flow approximation applied in the Conduit Flow Process (CFP) for MODFLOW-2005 mode 2, CFPM2. The good agreement between the experimental data and the simulated results verifies the utility of the approach used to simulate the effects of turbulent flow on head distributions and flux in the CFPM2 module of MODFLOW-2005. 相似文献
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Mitrovic SM Hamilton B McKenzie L Furey A James KJ 《Marine environmental research》2005,60(3):397-401
The change in concentration of the disulfated polyether yessotoxin (YTX) produced by a culture of the marine dinoflagellate Protoceratium reticulatum was measured in laboratory experiments under light and dark conditions. Experimental cultures were inoculated and grew at a growth rate of 0.14 d(-1) until stationary phase was reached, after approximately 21 days. Cultures were maintained in the stationary phase until 31 days after inoculation. Cells of P. reticulatum contained a concentration of approximately 10-15 pg YTX cell(-1) during stationary phase but this was considerably lower (<5 pg cell-1) during the growth phase. Low amounts of 45-hydroxy-YTX were also detected. At day 32, P. reticulatum was killed by cooling to 1 degrees C (confirmed microscopically) and YTX concentrations were measured periodically under light and dark conditions. YTX concentrations decreased rapidly to approximately 10% of the initial concentration within the first 3 days and depleted to near zero within a week in the light treatment. In the dark environment, YTX persisted longer with approximately 10% of the initial YTX concentration still remaining after 18 days. 相似文献
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Physiological tolerances limit the distribution of marine species, with geographical ranges being set by environmental factors, such as temperature and salinity, which affect the rates of vital processes and survival of marine ectotherms. The physiological tolerances of the non-native marine amphipod Caprella mutica were investigated in laboratory experiments. Adult C. mutica were collected from a fish farm on the west coast of Scotland and exposed to a range of temperatures and salinities for 48 h. C. mutica were tolerant of a broad range of temperature and salinity conditions, with 100% mortality at 30 degrees C (48 h LT50, 28.3+/-0.4 degrees C), and salinities lower than 16 (48 h LC50, 18.7+/-0.2). Although lethargic at low temperatures (2 degrees C), no mortality was observed, and the species is known to survive at temperatures as low as -1.8 degrees C. The upper LC(50) was greater than the highest salinity tested (40), thus it is unlikely that salinity will limit the distribution of C. mutica in open coastal waters. However, the species will be excluded from brackish water environments such as the heads of sea lochs or estuaries. The physiological tolerances of C. mutica are beyond the physical conditions experienced in its native or introduced range and are thus unlikely to be the primary factors limiting its present distribution and future spread. 相似文献