Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure   总被引：3，自引：0，他引：3  

Hayes RA  Regondi S  Winter MJ  Butler PJ  Agradi E  Taylor EW  Kevin Chipman J 《Marine environmental research》2004,58(2-5):665-669

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.  相似文献   

A Block-Based Theoretical Model Suited to Gravitational Sliding     

Tinti  Stefano  Bortolucci  Elisabetta  Vannini  Cesare 《Natural Hazards》1997,16(1):1-28

An original theoretical model has been devised to simulate mass flow over hill slopes due to gravitational sliding. The sliding mass is discretized into a sequence of contiguous blocks which are subjected to gravitational forces, to bottom friction and to surface resistance stresses that are generally negligible for subaerial flows, but are relevant for submarine slides. The blocks interact with each other while sliding down the hill flanks because of internal forces that dissipate mechanical energy and produce a momentum exchange between the individual blocks, yet conserving the total momentum of the mass. Internal forces are expressed in terms of interaction coefficients depending on the instantaneous distance between the block centers of mass, which is a measure of the deformation experienced by the blocks: the functional dependence includes three parameters, namely the interaction intensity ¯, the deformability parameter and the shape parameter , by means of which a wide range of interaction types can be fully accounted for. The time integration is performed numerically by solving the equations for the block velocities and positions at any time ti by means of the block accelerations at the previous time t_i-1, and by subsequently updating the block accelerations, which allows to proceed iteratively to the following times. The model has been tested against laboratory results available from literature and by means of several numerical experiments involving a simplified geometry both for the sliding body and the basal surface, with the purpose of clarifying the influence of the model parameters on the slide dynamics. The model improves the performance of the existing kinematic models for slides, moreover preserving an equivalent numerical simplicity. Future applications and possible improvements of this model are suggested.  相似文献   

An extended investigation of Helios 1 and 2 observations: The interplanetary magnetic field between 0.3 and 1 AU     

F. Mariani  U. Villante  R. Bruno  B. Bavassano  N. F. Ness 《Solar physics》1979,63(2):411-421

Helios-1 and 2 spacecraft allowed a detailed investigation of the radial dependence of the interplanetary magnetic field components between 0.3 and 1 AU. The behaviour of the radial component B _ris in a very good agreement with Parker's model (B _r r ^-2) and the azimuthal component B also shows a radial dependence which is close to theoretical predictions (B r ^-1). Experimental results for the normal component B and for the field magnitude B are consistent with those from previous investigations. The relative amplitude of the directional fluctuations with periods less than 12 hr is essentially independent of heliocentric distance, while their power decreases approximately as r ^–3 without any appreciable difference between higher and lower velocity regimes.Also at Laboratorio Plasma nello Spazio, CNR, Frascati.  相似文献   

Atmospheric nitrogen inputs into the North Sea: effect on productivity     

Gerrit de Leeuw  Lucinda Spokes  Tim Jickells  Carsten Ambelas Skjth  Ole Hertel  Elisabetta Vignati  Susanne Tamm  Michael Schulz  Lise-Lotte Srensen  Britta Pedersen  Laura Klein  K. Heinke Schlünzen 《Continental Shelf Research》2003,23(17-19):1743

The ANICE (Atmospheric Nitrogen Inputs into the Coastal Ecosystem) project addressed the atmospheric deposition of nitrogen to the North Sea, with emphasis on coastal effects. ANICE focused on quantifying the deposition of inorganic nitrogen compounds to the North Sea and the governing processes. An overview of the results from modelling and experimental efforts is presented. They serve to identify the role of the atmosphere as a source of biologically essential chemical species to the marine biota. Data from the Weybourne Atmospheric Observatory (UK) are used to evaluate the effect of short episodes with very high atmospheric nitrogen concentrations. One such episode resulted in an average deposition of 0.8 mmol N m⁻² day⁻¹, which has the potential to promote primary productivity of 5.3 mmol C m⁻² day⁻¹. This value is compared to long-term effects determined from model results. The total calculated atmospheric deposition to the North Sea in 1999 is 948 kg N km⁻¹, i.e. 0.19 mmol N m⁻² day⁻¹ which has the potential to promote primary productivity of 1.2 mmol C m⁻² day⁻¹. Detailed results for August 1999 show strong gradients across the North Sea due to adjacent areas where emissions of NO_x and NH₃ are among the highest in Europe. The average atmospheric deposition to the southern part of the North Sea in August 1999 could potentially promote primary production of 2.0 mmol C m⁻² day⁻¹, i.e. 5.5% of the total production at this time of the year in this area of the North Sea. For the entire study area the atmospheric contribution to the primary production per m² is about two-third of this value. Most of the deposition occurs during short periods with high atmospheric concentrations. This atmospheric nitrogen is almost entirely anthropogenic in origin and thus represents a human-induced perturbation of the ecosystem.  相似文献   

Large wood transport modelling by a coupled Eulerian–Lagrangian approach     

Elisabetta Persi  Gabriella Petaccia  Stefano Sibilla 《Natural Hazards》2018,91(1):59-74

The paper discusses a model which predicts the trajectory of floating rigid bodies and may be applied to compute the motion of woody “debris” mobilized during floods. The model couples a Discrete Element (DE) Lagrangian approach for the calculation of motion of rigid bodies with the Eulerian solution of the shallow water equations (SWE), in order to simulate the transport of a cylinder in a two-dimensional stream. It differs from existing models since it is based on a dynamic approach, adapting the Basset–Boussinesq–Oseen equation. In a first step, forces are computed from flow and log velocities; then, the equations of dynamics are solved to model the planar roto-translation of the body. Model results and physical reliability are clearly affected by the values of the drag and side coefficients, especially since logs, modelled as cylinders, are able to change their orientation towards the flow. Experimental studies to evaluate drag and side coefficients can be found in the literature for a submerged cylinder, with various orientations. To extend such results to the case of a floating log, the authors performed a series of laboratory tests on partially submerged cylinders, implementing the outcomes in the proposed DE-SWE model. The coupled model is validated against existing laboratory data concerning spheres and wooden cylinder transport.  相似文献   

Clinopyroxenes from mantle-related xenocrysts in alkaline basalts from Hannuoba (China): augite–pigeonite exsolutions and their thermal significance     

Elisabetta?Brizi  Sabrina?NazzareniEmail author  Francesco?Princivalle  Pier?Francesco?Zanazzi 《Contributions to Mineralogy and Petrology》2003,145(5):578-584

Optically homogeneous augite xenocrysts, closely associated with spinel–peridotite nodules, occur in alkali basalts from Hannuoba (Hebei province, China). They were studied by electron and X-ray diffraction to define the occurrence and significance of pigeonite exsolution microtextures. Sub-calcic augite (Wo₃₄) exsolved into En_62–62Fs_25–21Wo_13–17 pigeonite and En_46–45Fs_14–14Wo_40–42 augite, as revealed by TEM through diffuse coarser (001) lamellae (100–300 Å) and only incipient (100) thinner ones (<70 Å). C2/c augite and P2₁/c pigeonite lattices, measured by CCD-XRD, relate through a(Aug)?a(Pgt), b(Aug)?b(Pgt), c(Aug)≠c(Pgt) [5.278(1) vs 5.189(1)Å] and β(Aug)≠β(Pgt) [106.55(1) vs 108.55(2)°]. Cell and site volumes strongly support the hypothesis that the augite xenocrysts crystallised at mantle depth from alkaline melts. After the augite xenocrysts entered the magma, (001) lamellae first formed by spinodal decomposition at a T_min of about 1,100 °C, and coarsened during very rapid transport to the surface; in a later phase, possibly on cooling, incipient (100) lamellae then formed.  相似文献   

Natural background levels and threshold values derivation for naturally As, V and F rich groundwater bodies: a methodological case study in Central Italy     

Elisabetta Preziosi  Giuseppe Giuliano  Raffaella Vivona 《Environmental Earth Sciences》2010,61(5):885-897

Natural background levels (NBL) and threshold values (TV) derivation is an important task for EU Member States to correctly assess the chemical status of groundwater bodies within the context of the recently adopted Groundwater Directive. Attempts are being made both by water authorities and scientific community to test application viability of proposed methods to different real situations and to develop tailored adjustments and refinements. The aim of this paper is to describe a methodological case study of NBL and TV determination in a large area (5,500 km²) located in Central Italy where both natural and anthropogenic contamination occurs, resulting in several substances be detected in ground waters with concentration frequently exceeding drinking water standards. This paper shows the key role played in the determination process by knowledge on the hydrogeological setting and hydrogeochemical features. The adoption of appropriate pre-selection criteria of samples, the establishment of TV at the groundwater body scale for natural origin substances, and the evaluation of TVs exceedances in a multiparameter context are experimentally discussed. Beyond the critical evaluation of NBL and TV determination method, this research aims at increasing consciousness in the water management agencies that groundwater status assessment should be conceived as a scientific evaluation procedure and not as a mere technical/administrative exercise to be performed using any set of available data. As such, it should be supported by adequate knowledge of hydrogeological setting, hydrochemical processes occurring in groundwater bodies, and human activities acting overland to be progressively verified.  相似文献   

Principal-component analysis of RR Lyrae light curves     

S. M. Kanbur  H. Mariani 《Monthly notices of the Royal Astronomical Society》2004,355(4):1361-1368

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Search for long term variations of the interplanetary magnetic field     

F. Mariani  L. Diodato  G. Moreno 《Solar physics》1975,45(1):241-254

A statistical study is made of the long term variations of the interplanetary magnetic field parameters collected in the years 1964 to 1973 by 12 spacecraft (IMP's, Pioneers and HEOS). Although temporal fluctuations are observed on field components and magnitudes no clear solar cycle variation is found. The same conclusion holds for the statistical distributions and variances of these parameters. A search for possible heliographic latitude effects on the field also leads to a negative conclusion.  相似文献   

Chemical characterization of fine particulate matter during peak PM10 episodes in Apulia (South Italy)     

Martino Amodio  Paolo Bruno  Maurizio Caselli  Gianluigi de Gennaro  Paolo Rosario Dambruoso  Barbara Elisabetta Daresta  Pieirna Ielpo  Francesco Gungolo  Claudia Marcella Placentino  Vincenzo Paolillo  Maria Tutino 《Atmospheric Research》2008,90(2-4):313-ICNAA07

Some peak PM10 episodes, occurred during PM monitoring campaigns performed on October 2005 and February and June 2006 in Bari town, have been characterized. Moreover back trajectories of air masses and Principal Component Analyses were applied. Three of the peak PM10 episodes investigated were related to local emissions of primary pollutants during poor atmospheric dispersion conditions. The other two peak PM10 episodes considered are related with long range transport air masses toward Apulia region: in one case the chemical characterization and the back trajectories analysis indicate that high PM10 value detected is due to the Saharan dust advection in the Apulia region; in the other case air masses with different origin give rise to high PM10 value.Moreover PM10 daily mean concentrations, presented in this paper collected from January 2005 to August 2007 and obtained by automatic device in six stations of air quality monitoring networks in Bari territory, do not show a seasonal trend for PM10 concentrations, contrary to the PM10 trend shown in the towns of North Italy. This can be explained mostly considering that our region presents generally meteo-climatic conditions that favour pollutants dispersion.  相似文献