Effects of water-borne copper on metallothionein and lipid peroxidation in the marine amphipod Gammarus locusta   总被引：1，自引：0，他引：1  

Correi AD  Livingstone DR  Costa MH 《Marine environmental research》2002,54(3-5):357-360

The aim of the current work was to determine over 10 days the effects of water-borne exposure of the marine amphipod Gammarus locusta to 4 microgCu l(-1) on the metallothionein (MT; measured by differential pulse polarography) protection system and lipid peroxidation (LP: thiobarbituric acid-reactive malondialdehyde equivalents) as a measure of oxidative damage. MT levels in exposed animals increased significantly at day 2 (36% > control; P < 0.001) and remained high at days 6 and 10 (55 and 38%, respectively, P < 0.001). The maximum level of MT at day 6 coincided with the highest Cu body-burden. LP increased within I day of exposure, indicative of Cu as an oxidative stressor. However, in contrast to MT, the highest LP level was seen at day 4 (68% > control, P < 0.001) before returning to control values by day 6, indicating a protective role of MT against the pro-oxidant effects of Cu.  相似文献   

Microphytobenthos vertical migratory photoresponse as characterised by light-response curves of surface biomass     

Joo Serdio  Helena Coelho  Snia Vieira  Snia Cruz 《Estuarine, Coastal and Shelf Science》2006,68(3-4):547

The migratory response of intertidal microphytobenthos to changes in irradiance was studied on undisturbed estuarine sediments. Two non-destructive optical techniques were used to trace variations in vivo of surface biomass: PAM fluorometry, for measuring the minimum fluorescence level (F_o); and spectral reflectance analysis, for quantifying the normalized difference vegetation index (NDVI). Following the formation of a dense biofilm at the surface, replicated sediment samples were simultaneously exposed to six different irradiance levels, ranging from 50 to 1500 μmol m⁻² s⁻¹, during a period of 120 min. The migratory photoresponse of the biofilms was characterised by constructing biomass vs. light curves (BLC), relating the accumulation of microalgal biomass after that period (estimated by F_o or NDVI) to the irradiance level incident on the surface. BLCs allow characterising the main features of the migratory photoresponse of intact biofilms. Typical BLC showed a clear biphasic pattern, with an increase in microalgal accumulation under irradiances below 100 μmol m⁻² s⁻¹, maximum values under 100–250 μmol m⁻² s⁻¹, and a gradual decrease of surface biomass under higher irradiances, indicating a strong photophobic downward migratory response. Similar BLC patterns were obtained when measuring F_o or NDVI. The construction of BLCs for biofilms from intertidal sites with distinctive sediment characteristics and diatom taxonomic composition allowed to detected significant differences in the migratory photoresponse. Biofilms from a muddy sediment exhibited considerably larger amplitude in the migratory photoresponse than the biofilms from a sandy mud site, especially under high irradiances. The photophobic migratory response to high light was found to vary among diatom species, particularly in the case of the biofilms from the muddy sediments.  相似文献   

Multi-level assessment of chronic toxicity of estuarine sediments with the amphipod Gammarus locusta: II. Organism and population-level endpoints     

Costa FO  Neuparth T  Correia AD  Costa MH 《Marine environmental research》2005,60(1):93-110

This study aimed to test the performance of the amphipod Gammarus locusta (L.) in chronic sediment toxicity tests. It constitutes part of a multi-level assessment of chronic toxicity of estuarine sediments, integrating organism and population-level endpoints with biochemical markers responses. Here we account for organism and population-level effects, while biomarker responses were reported in a companion article. Five moderately contaminated sediments from Sado and Tagus estuaries were tested, comprising 3 muddy and 2 sandy sediments. These sediments either did not show acute toxicity or were diluted with control sediment as much as required to remove acute toxicity. Subsequent chronic tests consisted of 28-day exposures with survival, individual growth and reproductive traits as endpoints. Two of the muddy sediments induced higher growth rates in the amphipods, and improved reproductive traits. This was understood to be a consequence of the amount of organic matter in the sediment, which was nutritionally beneficial to the amphipods, while concurrently decreasing contaminant bioavailability. Biomarker responses did not reveal toxicant-induced stress in amphipods exposed to these sediments. One of the sandy sediments was acutely toxic at 50% dilution, but in contrast stimulated amphipod growth when diluted 75%. This was presumed to be an indication of a hormetic response. Finally the two remaining contaminated sediments showed pronounced chronic toxicity, affecting survival and reproduction. The sex ratio of survivors was highly biased towards females, and offspring production was severely impaired. The particulars of the responses of this amphipod were examined, as well as strengths versus limitations of the sediment test. This study illustrates the utility of this chronic test for toxicity assessment of contaminated estuarine sediments, with potential application all along Atlantic Europe.  相似文献   

Interpolation by regularized spline with tension: I. Theory and implementation   总被引：3，自引：0，他引：3  

Helena Mitáová  Lubos Mitá 《Mathematical Geology》1993,25(6):641-655

Bivariate and trivariate functions for interpolation from scattered data are derived. They are constructed by explicit minimization of a general smoothness functional, and they include a tension parameter that controls the character of the interpolation function (e.g., for bivariate case the surface can be tuned from a membrane to a thin steel plate), Tension can be applied also in a chosen direction, for modeling of phenomena with a simple type of anisotropy. The functions have regular derivatives of all orders everywhere. This makes them suitable for analysis of surface geometry and for direct application in models where derivatives are necessary. For processing of large datasets (thousands of data points), which are now common in geosciences, a segmentation algorithm with a flexible size of overlapping neighborhood is presented. Simple examples demonstrating flexibility and accuracy of the functions are presented.On leave from the Department of Physical Geography and Cartography, Comenius University, Mlynská dolina, Bratislava, Czechoslovakia.On leave from the Institute of Physics, Dúbravská cesta 9, Bratislava, Czechoslovakia.  相似文献   

New Labour, new geography? The electoral geography of the 1997 British General Election     

Charles Pattie  Ron Johnston  Danny Dorling  Dave Rossiter  Helena Tunstall  Iain MacAllister 《Area》1997,29(3):253-259

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GRASS as Open Source Free Software GIS: Accomplishments and Perspectives     

Helena Mitasova  Markus Neteler 《Transactions in GIS》2004,8(2):145-154

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Geospatial analysis of a coastal sand dune field evolution: Jockey's Ridge, North Carolina     

Helena Mitasova  Margery Overton  Russell S. Harmon 《Geomorphology》2005,72(1-4):204-221

Preservation and effective management of highly dynamic coastal features located in areas under development pressures requires in-depth understanding of their evolution. Modern geospatial technologies such as lidar, real time kinematic GPS, and three-dimensional GIS provide tools for efficient acquisition of high resolution data, geospatial analysis, feature extraction, and quantification of change. These techniques were applied to the Jockey's Ridge, North Carolina, the largest active dune field on the east coast of the United States, with the goal to quantify its deflation and rapid horizontal migration. Digitized contours, photogrammetric, lidar and GPS point data were used to compute a multitemporal elevation model of the dune field capturing its evolution for the period of 1974– 2004. In addition, peak elevation data were available for 1915 and 1953. Analysis revealed possible rapid growth of the dune complex between 1915–1953, followed by a slower rate of deflation that continues today. The main dune peak grew from 20.1 m in 1915 to 41.8 m in 1953 and has since eroded to 21.9 m in 2004. Two of the smaller peaks within the dune complex have recently gained elevation, approaching the current height of the main dune. Steady annual rate of main peak elevation loss since 1953 suggests that increase in the number of visitors after the park was established in 1974 had little effect on the rate of dune deflation. Horizontal dune migration of 3–6 m/yr in southerly direction has carried the sand out of the park boundaries and threatened several houses. As a result, the south dune section was removed and the sand was placed at the northern end of the park to serve as a potential source. Sand fencing has been an effective management strategy for both slowing the dune migration and forcing growth in dune elevation. Understanding the causes of the current movements can point to potential solutions and suggest new perspectives on management of the dune as a tourist attraction and as a recreation site, while preserving its unique geomorphic character and dynamic behavior.  相似文献   

Numerical modeling of fracking fluid migration through fault zones and fractures in the North German Basin     

Helena Pfunt  Georg Houben  Thomas Himmelsbach 《Hydrogeology Journal》2016,24(6):1343-1358

Gas production from shale formations by hydraulic fracturing has raised concerns about the effects on the quality of fresh groundwater. The migration of injected fracking fluids towards the surface was investigated in the North German Basin, based on the known standard lithology. This included cases with natural preferential pathways such as permeable fault zones and fracture networks. Conservative assumptions were applied in the simulation of flow and mass transport triggered by a high pressure boundary of up to 50 MPa excess pressure. The results show no significant fluid migration for a case with undisturbed cap rocks and a maximum of 41 m vertical transport within a permeable fault zone during the pressurization. Open fractures, if present, strongly control the flow field and migration; here vertical transport of fracking fluids reaches up to 200 m during hydraulic fracturing simulation. Long-term transport of the injected water was simulated for 300 years. The fracking fluid rises vertically within the fault zone up to 485 m due to buoyancy. Progressively, it is transported horizontally into sandstone layers, following the natural groundwater flow direction. In the long-term, the injected fluids are diluted to minor concentrations. Despite the presence of permeable pathways, the injected fracking fluids in the reported model did not reach near-surface aquifers, either during the hydraulic fracturing or in the long term. Therefore, the probability of impacts on shallow groundwater by the rise of fracking fluids from a deep shale-gas formation through the geological underground to the surface is small.  相似文献   

Structural and Functional Composition of Benthic Nematode Assemblages During a Natural Recovery Process of <Emphasis Type="Italic">Zostera noltii</Emphasis> Seagrass Beds     

Patrick Materatski  Anna-Maria Vafeiadou  Tom Moens  Helena Adão 《Estuaries and Coasts》2016,39(5):1478-1490

In 2008, the stable seagrass beds of the Mira estuary (SW Portugal) disappeared completely; however, during 2009, they have begun to present early symptoms of natural recovery, characterised by a strongly heterogeneous distribution. This study was designed to investigate the spatial and temporal variability patterns of species composition, densities and trophic composition of the benthic nematode assemblages in this early recovery process, at two sampling sites with three stations each and at five sampling occasions. Because of the erratic and highly patchy seagrass recovery and the high environmental similarity of the two sampling sites, we expected within-site variability in nematode assemblages to exceed between-site variability. However, contrary to that expectation, whilst nematode genus composition was broadly similar between sites, nematode densities differed significantly between sites, and this between-site variability exceeded within-site variability. This may be linked to differences in the Zostera recovery patterns between both sites. In addition, no clear temporal patterns of nematode density, trophic composition and diversity were evident. Nematode assemblages generally resembled those of other estuarine muddy intertidal areas, which have a high tolerance of stress conditions.  相似文献   

Ecotoxicological evaluation of dredged sediments from Rodrigo de Freitas Lagoon (Rio de Janeiro State,Brazil) using bioassays with earthworms and collembolans     

Mariana Vezzone  Ricardo Cesar  Helena Polivanov  Aline Serrano  Danielle Siqueira  Leticia Abreu  Miriam Bianchi  Maria Elizabeth Correia  Zuleica Castilhos  Tácio de Campos 《Environmental Earth Sciences》2018,77(21):743

The toxicity and metal bioavailability were studied in dredged sediments from Rodrigo de Freitas Lagoon (Rio de Janeiro, RJ, Brazil) using acute and avoidance tests with Eisenia andrei, and reproduction tests with Folsomia candida. The sediment was mixed with an artificial soil, and two natural soils (ferralsol and chernosol—representative Brazilian tropical soils) to obtain the following doses: 1, 3, 6, 12, 18, 24 and 30%. Total metal concentrations were determined in the sediment to support the interpretation of ecotoxicological data. Metal concentrations in the mixtures were in agreement with the threshold limits established by Brazilian law. However, significant avoidance responses were found on doses ≥?3% and were the most sensitive endpoint. Earthworm mortality found in artificial soil mixtures (LC50?=?3.9) suggests higher toxicity levels than those obtained in ferralsol (LC50?=?7.6%) and chernosol (11.0%) treatments. Earthworm mortality, avoidance responses and collembolan reproduction levels found in ferralsol mixtures (LC50?=?9.2; avoidance EC50?=?2.3%; reproduction EC50?=?2.8%) were higher compared to chernosol treatments (LC50?=?11.0%; avoidance EC50?=?4.3%; reproduction EC50?=?4.9%). The reduction of toxicity levels in chernosol mixtures is probably due to the abundance of expansive clay minerals in chernosols with capacity of adsorbing metals and other xenobiotic substances from soil pore water, decreasing metal bioavailability. Finally, threshold limits defined by Brazilian legislation for soil quality and land disposal of dredged sediments are not sufficient to prevent noxious effects on soil fauna and should be complemented with a preliminary ecotoxicological evaluation.  相似文献