Correlative changes in metabolism and DNA damage in turbot (Scophthalmus maximus) exposed to benzo[a]pyrene     

Telli-Karakoç F  Ruddock PJ  Bird DJ  Hewer A  Van Schanke A  Phillips DH  Peters LD 《Marine environmental research》2002,54(3-5):511-515

Juvenile turbot (Scophthalmus maximus) were injected intraperitoneally with either corn oil or 5 mg/kg benzo[a]pyrene (BaP) dissolved in corn oil and sampled I and 3 days after injection. After 1 day, no elevation of 7-ethoxyresorufin O-deethylase (EROD) activity was observed, however bile metabolites (BaP-7,8 dihydrodiol representing 70% of the total metabolites) and a single hepatic DNA adduct spot (0.47 adducts/10(8) nucleotides) identified by 32P-postlabelling were formed. No BaP metabolites or DNA adducts were observed in either control or carrier control fish. Fish sampled after 3 days reported 5-fold higher (P < 0.05) levels of EROD activity, a shift in the bile metabolite profile towards BaP phenol formation (1OH and 30H BaP comprising up to 60% of total metabolites detected) and the formation of two adduct spots (0.86 and 0.71 adducts/10(8) nucleotides). These results show that BaP can be metabolised and form hydrophobic DNA adducts in turbot without EROD elevation. Following EROD elevation, a shift in the profile of both BaP metabolites and BaP metabolite-DNA interactions occurs indicative of other oxidative processes.  相似文献   

Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure   总被引：3，自引：0，他引：3  

Hayes RA  Regondi S  Winter MJ  Butler PJ  Agradi E  Taylor EW  Kevin Chipman J 《Marine environmental research》2004,58(2-5):665-669

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.  相似文献   

Sampling strategies to evaluate the status of offshore soft sediment assemblages     

Rogers SI  Somerfield PJ  Schratzberger M  Warwick R  Maxwell TA  Ellis JR 《Marine pollution bulletin》2008,56(5):880-894

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Multiscale characterization of a heterogeneous aquifer using an ASR operation   总被引：1，自引：0，他引：1  

Pavelic P  Dillon PJ  Simmons CT 《Ground water》2006,44(2):155-164

Heterogeneity in the physical properties of an aquifer can significantly affect the viability of aquifer storage and recovery (ASR) by reducing the recoverable proportion of low-salinity water where the ambient ground water is brackish or saline. This study investigated the relationship between knowledge of heterogeneity and predictions of solute transport and recovery efficiency by combining permeability and ASR-based tracer testing with modeling. Multiscale permeability testing of a sandy limestone aquifer at an ASR trial site showed that small-scale core data give lower-bound estimates of aquifer hydraulic conductivity (K), intermediate-scale downhole flowmeter data offer valuable information on variations in K with depth, and large-scale pumping test data provide an integrated measure of the effective K that is useful to constrain ground water models. Chloride breakthrough and thermal profiling data measured during two cycles of ASR showed that the movement of injected water is predominantly within two stratigraphic layers identified from the flowmeter data. The behavior of the injectant was reasonably well simulated with a four-layer numerical model that required minimal calibration. Verification in the second cycle achieved acceptable results given the model's simplicity. Without accounting for the aquifer's layered structure, high precision could be achieved on either piezometer breakthrough or recovered water quality, but not both. This study demonstrates the merit of an integrated approach to characterizing aquifers targeted for ASR.  相似文献   

Ideal width of transects for monitoring source-related categories of plastics on beaches     

de Araújo MC  Santos PJ  Costa MF 《Marine pollution bulletin》2006,52(8):957-961

Although there is a consensus on the necessity of monitoring solid wastes pollution on beaches, the methods applied vary widely. Therefore, creating, testing and recommending a method that not only allows comparisons of places and periods, but also the detection of source signals, will be important to reach the objectives of the source-prevention principle. This will also allow the optimisation of time, resources, and processing of samples and data. A classification of the items found into specific categories was made according to their most probable source/use (fisheries, food packaging, hazardous, sewage/personal hygiene, beach user, general home). This study tested different widths of sampling transects to be used in the detection of plastics contamination on beaches, until all the categories were significantly represented. Each transect had its total width (50m) sub-divided into eight intervals of 0-2.5m; 2.5-5m; 5-10m; 10-15m; 15-20m; 20-30m; 30-40m; and 40-50m. The accumulated number of categories in the 50m (up to 2.5m; up to 5m and so on) was used to determine the minimal width necessary to qualitatively characterize the area regarding plastics contamination. The diversity of the categories was directly related to the area of the sampling transect. These results indicate that a significant increase in the number of categories in the first intervals tend to stabilize from 15-20m onwards.  相似文献   

Measurements of biomarker levels in flounder (Platichthys flesus) and blue mussel (Mytilus trossulus) from the Gulf of Gdańsk (southern Baltic)     

Kopecka J  Lehtonen KK  Barsiene J  Broeg K  Vuorinen PJ  Gercken J  Pempkowiak J 《Marine pollution bulletin》2006,53(8-9):406-421

In the framework of the EU funded BEEP project a set of biomarkers, gross morphometric indices and tissue concentrations of selected organic pollutants were measured in flounder (Platichthys flesus) and mussels (Mytilus trossulus) collected twice a year (April and October) from three sites in the inner Gulf of Gdańsk between autumn 2001 and spring 2003. In flounder, seasonal differences in most biomarkers were observed, but no correlations with tissue pollutant levels could be found. In mussels, highly variable levels in biomarker responses were seen, but no clear seasonal or spatial trends, directly related to tissue concentrations, could be established. The observed biomarkers distribution the study sites are probably mostly caused by interannual, seasonal and individual variability and, in case of flounder, possibly by exchange of stocks between the sampling sites.  相似文献   

Use of biliary PAH metabolites as a biomarker of pollution in fish from the Baltic Sea   总被引：2，自引：0，他引：2  

Vuorinen PJ  Keinänen M  Vuontisjärvi H  Barsiene J  Broeg K  Förlin L  Gercken J  Kopecka J  Köhler A  Parkkonen J  Pempkowiak J  Schiedek D 《Marine pollution bulletin》2006,53(8-9):479-487

During field campaigns of the BEEP project (Biological Effects of Environmental Pollution in Marine Coastal Ecosystems) in 2001-2002, metabolites of polycyclic aromatic hydrocarbons (PAHs) were determined in bile samples from three fish species, flounder (Platichthys flesus), perch (Perca fluviatilis) and eelpout (Zoarces viviparus), from four separate areas in the Baltic Sea. Two determination methods were applied: fixed wavelength fluorescence (FF) for pyrene-type metabolites and high-performance liquid chromatography with fluorescence detection (HPLC). There was a good correlation between the FF method and 1-OH pyrene determined by HPLC. Normalisation of the FF data for absorbance at 380 nm or bile protein concentrations greatly increased variance in one third and decreased it in two thirds of the cases and resulted in a loss of significant differences (protein normalisation) between the sampling stations, but normalisation of the HPLC data had little effect on the results. The biliary PAH metabolite content was usually higher in males than in females. In perch and eelpout the biliary PAH contents were at similar levels, whereas in flounder the levels were lower. The sampling areas arranged in decreasing order of biliary PAH contents were: Wismar Bay > Gulf of Gdansk > Lithuanian coast > Kvadofj?rden (reference area). It is concluded that FF with un-normalised data is a reliable and simple method for monitoring purposes and only one sex of a selected species should be used.  相似文献   

大西洋中脊超镁铁质岩中热液硫化物中的合金—铜组合     

Murp  PJ Meyer  G 《贵金属地质》2000,9(3):178-183

发育于超镁铁质岩基底上的Ｌｏｇａｔｃｈｅｖ热液活动区的硫化物,表现出富铜和富锌矿物组合的双峰式分布特点,二者均有较高的金含量。富铜的火山口号有现为一种不同寻常的洼地地貌,少有筒状结构。富铜样品显示出主要为金－铜矿物组合以及小规模交代和再造作用过程中金富集的证据。火山口地貌、Ａｕ／Ａｇ值以及硫化作用状态表明,在富铜样品中,金不是以Ａｕ（ＳＨ）２＾－的形式运移的。  相似文献   

A Model for Structure Formation Seeded by Gravitationally Produced Matter     

Hu W  Peebles PJ 《The Astrophysical journal》2000,530(2):L61-L64

The Arecibo H i Strip Survey probed the halos of approximately 300 cataloged galaxies and the environments of approximately 14 groups with sensitivity to neutral hydrogen masses >/=107 M middle dot in circle. The survey detected no objects with properties resembling the high-velocity clouds (HVCs) associated with the Milky Way or Local Group. If the HVCs were typically MHi=107.5 M middle dot in circle objects distributed throughout groups and galaxy halos at distances of approximately 1 Mpc, the survey should have made approximately 70 HVC detections in groups and approximately 250 detections around galaxies. The null detection implies that HVCs are deployed at typical distances of 相似文献   

Resolving mechanisms of toxicity while pursuing ecotoxicological relevance?     

Hinton DE  Kullman SW  Hardman RC  Volz DC  Chen PJ  Carney M  Bencic DC 《Marine pollution bulletin》2005,51(8-12):635-648

In this age of modern biology, aquatic toxicological research has pursued mechanisms of action of toxicants. This has provided potential tools for ecotoxicologic investigations. However, problems of biocomplexity and issues at higher levels of biological organization remain a challenge. In the 1980s and 1990s and continuing to a lesser extent today, organisms residing in highly contaminated field sites or exposed in the laboratory to calibrated concentrations of individual compounds were carefully analyzed for their responses to priority pollutants. Correlation of biochemical and structural analyses in cultured cells and tissues, as well as the in vivo exposures led to the production and application of biomarkers of exposure and effect and to our awareness of genotoxicity and its chronic manifestations, such as neoplasms, in wild fishes. To gain acceptance of these findings in the greater environmental toxicology community, “validation of the model” versus other, better-established often rodent models, was necessary and became a major focus. Resultant biomarkers were applied to heavily contaminated and reference field sites as part of effects assessment and with investigations following large-scale disasters such as oil spills or industrial accidents.

Over the past 15 years, in the laboratory, small aquarium fish models such as medaka (Oryzias latipes), zebrafish (Danio rerio), platyfish (Xiphophorus species), fathead minnow (Pimephales promelas), and sheepshead minnow (Cyprinodon variegatus) were increasingly used establishing mechanisms of toxicants. Today, the same organisms provide reliable information at higher levels of biological organization relevant to ecotoxicology. We review studies resolving mechanisms of toxicity and discuss ways to address biocomplexity, mixtures of contaminants, and the need to relate individual level responses to populations and communities.  相似文献