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O-phthaldialdehyde is used for the determination of free amino acid in seawater by measuring the fluorescence produced by its reaction with the a-amino group of amino acids. This method is so sensitive that as little as 1-10 nM of amino acids and less than 1 ml of seawater may be sufficient for one test.This method is simple, rapid and accurate and a-amino acids contained in seawater can directly be determined without concentration and extraction. It is free from the interference of large content of inorganic salts and other nitrogenous compounds in seawater.A hundred and fifty-two samples collected at 24 stations in offshore of Zhejiang have been analyzed. The results obtained show that the range of concentration of the total free amino acid is 0.37-4.70 μM. The values are high near shore and low offshore.  相似文献
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