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几种红藻和蓝藻的光合作用色素 总被引：2，自引：0，他引：2
分离纯化出几种海产红藻和一种蓝藻的光合作用色素,并测定了它们的化学性质和光谱学性质。这些藻类是3种红藻:多管藻(Polysiphonia urceolata)、橡叶藻(Phycodrys sp.)和条斑紫菜(Porphyra yezoensis);蓝藻:钝顶螺旋藻(Spirulina platensis)。用羟基磷灰石柱层析法从上述藻类中分离到几种不同的藻胆蛋白。经SDS-PAGE及光谱测定,发现条斑紫菜中的藻红蛋白不同于其它两种红藻。而橡叶藻中存在的两条藻红蛋白也有差异,条斑紫菜和钝顶螺旋藻中的两种别藻蓝蛋白之间也有区别。叶绿素分析表明,钝顶螺旋藻中叶绿素a的含量高于红藻中叶绿素a的含量。 相似文献
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几种海洋微藻的固定化培养 总被引：15，自引：2，他引：15
用褐藻酸钙固定化培养了7种海洋微藻,它们是等鞭金藻(Isochrysis galbana)、微形角刺藻(Chaetoccros minutissimus)、三角褐指藻(Phaeodactylum tricornutum)、青岛大扁藻(Platymonas helgolandica var.tsingtaoensis Tseng et T.J.Chang)、亚心形扁藻(Pl.subcordiformis)、隐杆藻(Aphanothece sp.)和盐藻(Dunaliella sp.). 相似文献
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固定化培养对亚心形扁藻生理功能及超微结构的影响 总被引：6，自引：1，他引：6
以亚心形扁藻(Platymonas subcordiformis)为材料,研究了2%(W/V)的褐藻胶包埋,用不同浓度的CaCl2(0.5、0.75、1.0、1.5及2.0mol/dm3)处理,在不同培养温度下(25℃、15℃)对细胞的生长、形态、光合作用及呼吸作用的影响.发现最佳固定化培养条件为CaCl2 0.5mol/dm3,培养温度为25℃.固定北培养的细胞有较高的光合作用速率.在其他条件相同的情况下,在15℃条件下比25℃条件下有较高的光合作用速率.扫描电镜观察表明,固定化培养的亚心形扁藻细胞变大,表面凹凸不平.本文还就固定化培养各个因子之间的关系及固定化培养的意义和应用前景进行了讨论. 相似文献
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几种红藻和蓝藻藻胆体的分离和光谱特性 总被引：3，自引：0，他引：3
本文分析了高等红藻龙须菜(Gracilaria lemaneiformis)藻胆体的吸收光谱和荧光光谱,并与低等红藻条斑紫菜(Porphyra yezoensis)及蓝藻钝顶螺旋藻(Spirulina platensis)作了比较研究。龙须菜藻胆体的吸收峰位于498nm、541nm、565nm、620nm和650nm。荧光发射峰位于576nm和672nm。从光谱学性质和能量传递分析,龙须菜和条斑紫菜胆体的组成、结构相似,但有细微差别。而螺旋藻藻胆体的组成、结构则与两种红藻差别较大。 相似文献
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Polyc . lonal antibodies raised against LHC II isolated from SDS-solubilized Bryopsis corticulans thylakiod membranes by SDS-PAGE, were characterised by double immunodifiusion, Rocket immunoelectrophoresis and antigen-antibody crossed immunoelectro - phoresis assays showed the antibodies had strong cross-reaction with all B , corticulans LHC II components (even with those which were incubated in boiling water)and showed immunological cross-reactivity with LHC II polypeptides of spinach and the marine green alga Codium fragile. The results suggested that LHC II of different species had similar antigenic determinants and also conservation of amino acid sequences of the polypeptides during evolution, and that the antibodies could cross react with apoproteins of D2 proteins (which contain P680) from B. corticulans, spinach and C. fragile, but not with apoproteins of P 700 Chl-proteins. Our results indicated some similarities in primary structure between LHC II of different species, and between LHC II and D2 相似文献
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P700 Chi-protein was isolated from a marine green alga Bryopsis corticulans with SDS-resolved thylakoid membranes by SDS-PAGE. After elution from the gel, the recovered protein revealed a 100 KD polypeptide by re-electrophoresis . The same SDS-PAGE procedure was used to isolate P700 Chl-proteins from spinach (Spinacea okracea ) and another marine green alga Codium fragile . Polyclonal antibodies to Bryopsis P700 protein were raised in rabbits. The antibodies were shown to cross-react with P700 Chl-protein and Chl-protein complexes containing P700 protein from B . Corticulans, C. f ragile, and even spinach. Results indicate similarity of the amino acid sequences of the P700 Chl-proteins and the highly conserved structure of the apoproteins of phylogenetically distant species over evolution. The antibodies cross-react with none of the components of PSUU in the species tested , indicating an independent pathway of evolution of photosystem I and photosystem II of later origin. 相似文献
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The excitation energy transfer from phycobiliproteins to thylakoid PSII of higher plants was investigated. When incubated with spinach thylakoids, phycobiliproteins isolated from red and blue- green algae transferred light energy absorbed to spinach PSII. The efficiency of energy transfer was dependent on the kind of phycobiliproteins used. If spinach thylakoids were replaced by the thylakoids of Brassica chinensis, R phycoerythin or C- phycocyanin did not transfer their excitation energy to PSII of Brassica chinensis unless allophycocyanin was present. 相似文献
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