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本研究采用大气压下强电离放电协同气液混溶技术,高效制备羟基自由基(·OH)杀灭3个门的典型有害赤潮物种,使用荧光染色、测定光合作用潜能等生物学检测方法确定·OH致死阈值。结果表明,5.05×104 cells/mL的赤潮异弯藻(Heterosigma akashiwo)、5.28×104 cells/mL的亚历山大藻(Alexandrium tamarense)和5.02×104 cells/mL的中肋骨条藻(Skeletonema costatum),其致死阈值分别为1.24 mg/L、2.01 mg/L、1.12 mg/L,此时其叶绿素a分解率分别为77%、85%和74%。利用光学显微镜观察,处理前后藻细胞结构有明显的改变。因此,·OH致死方法可有效地杀灭压载水中的有害赤潮藻。 相似文献
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The relationship between microsatellite polymorphism and body weight of captive bred Chinese sea cucumber Apostichopus japonicus was investigated in two local populations in Dalian. Among ten loci discovered, nine show changes except for AJ07 loci. Seven
loci were found highly polymorphic in both populations. For each locus in two populations, the average number of alleles is
6.428 6 and 6.285 7, the average observed heterozygosity at 0.225 7 and 0.245 9, the expected heterozygosity at 0.776 8 and
0.748 8, the polymorphism information content (PIC) at 0.709 2 and 0.674 6, respectively. Further analysis show significant
correlation between A. japonicus body weight and occurrence markers AJ02 and AJ04. The findings of the relation may be helpful for molecular breeding, as
well as the marker-assisted selection of sea cucumbers.
Supported by the National High Technology Research and Development Program of China (863 Program, No. 2006AA10A411), the Natural
Science Foundation of Liaoning Province (No.20072139), the Grant of Dalian Fisheries University, the Key Laboratory Foundation
of the Educational Department of Liaoning Province (No.2009S024) 相似文献
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