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INTRODUCTIONOverthepastdecade ,Haematococcuspluvialishasbeenasubjectofnumerousinvestigationsbe causeitsconspicuousredcolorpointstoitspossibilityasahopefulregenerativebioresourceofastaxan thin .Extensivestudyofculturefactors (suchastemperature,illuminatio…  相似文献   
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This study on dynamic changes of culture color,astaxanthin and chlorophylls,inorganic N including N-NO3^-,N-NO2^- and N-NH4^ in batch culture of Haematococcus pluvialis exposed to different additive nitrate concentration showed(1)ast/chl ratio was over 0.8 for brown and red algae,but was usually less than 0.5 for green and yellow algae;(2)N-NO3^-,in general,was unstable and decreased,except for a small unexpected increase in nitrate enriched treatment groups;(3)measurable amounts of N-NO2^- and N-NH4^ were observed respectively with three change modes although no extemal nitrite and ammonia were added into the culture;(4)a non-linear correlation between ast/chl ratio(or color)changes and the levels of N-NO3^-,N-NO2^-,N-NH4^ in H.pluvialis culture;(5)up and down variation of the ast/chl ratio occurred simultaneously with a perceptible color change from yellow to brown(or red)when N-NO3^-,N-NO2^- and N-NH4^ fluctuated around 30,5,5μmol/L respectively;(6)existence of three dynamic modes of N-NO3^-,N-NO2^- and N-NH4^ changes,obviously associated with initial extemal nitrate;(7)the key level of total inorganic N concentration regulating the above physiological changes during indoor cultivation was about 50 μmol/L;and(8)0.5-10mmol/L of nitrate was theoretically conducive to cell growth in batch culture.  相似文献   
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雨生红球藻的信号物质   总被引:15,自引:1,他引:14  
以单细胞雨生红球藻为材料,在室内通气培养条件下,研究了经该藻处理过的培养液内是否存在对细胞生长或转化产生影响的信号物质。对比研究结果表明,处理过的旧液明显不利于红球藻游动细胞生长,但有利于不动细胞增加。预显着旧液中可能存在着降低游动细胞生长、同时还促使游动细胞向不动细胞转化的信号物质。旧液中该信号物质的多少与原来处理旧液的藻细胞密度有密切关系,密度越大信号物质越多,对细胞生长和转化的作用也越强。  相似文献   
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光生物反应器的组建   总被引:1,自引:0,他引:1  
于1997年4月在借鉴国外资料基础上,组建成了一种适用于实验室培养光自养生物(如光合细菌,微藻和单克隆无性繁殖系)的气升式螺旋管道串联的光生物反应器,该装置内可插入pH值电极,溶解氧电极,温度电极,细胞密度电极,粘度电极等,通过传感器获得的电信号,进一步传导入微机,实现按程序化自动记录和控制有关参数,进行一次性,间歇和连续浮培养,以红球藻为材料进行培养和初步结果表明,培养时间较常规方法缩短了一半,  相似文献   
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