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温室气体过度排放引起的全球气候变化已经成为世界各国需要共同应对的首要环境问题,其中化石燃料燃烧产生的二氧化碳排放被认为是引起全球气候变暖的主要因素,所以减少二氧化碳排放可能是逆转全球气候变暖的主要措施。大规模微藻培养可以在减排二氧化碳的同时处理废水、 相似文献
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In 2008,a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao.The causative species was determined to be Enteromorpha prolifera (Ulva prolifera O.F.Müller),a familiar green macroalga along the coastline of China.Rapid accumulation of a large biomass of floating U.prolifera prompted research on different aspects of this species.In this study,we constructed a nonnormalized cDNA library from the thalli of U.prolifera and acquired 10 072 high-quality expressed sequence tags ... 相似文献
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A harmful algae bloom (HAB) is a dense aggregation of algae in a marine or aquatic environment that can result in significant environmental problems. To forecast the occurrence of HAB, development of a rapid and precise detection method is urgently required. In this study, two Skeletonema costatum-like diatoms (SK-1 and SK-2), were identified morphologically under a light microscope, and detected using fluorescent in situ hybridization (FISH). Strain SK-1 was isolated from a frequently HAB affected area of the East China Sea, and strain SK-2 from an aquatic farm in Qingdao, China. Fluorescent DNA probes were designed that were complementary to the ITS sequence (including 5.8S rDNA) of strain SK-1. After hybridization, strong green fluorescence was observed in cells of strain SK-1 under an epifluorescence microscope; however, no such fluorescence was observed with strain SK-2, which indicates that probes hybridized only the DNA of the target strain, SK-1, in species-specific manner, and that the two strains do not belong to a same species. This finding was confirmed by ITS sequence analysis. The FISH technique used in this study was sensitive, simple, and rapid, and is a promising tool for detecting target HAB species in natural environments. 相似文献
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不同碳氮浓度对雨生红球藻生长及虾青素累积的影响 总被引：1，自引：0，他引：1
研究了不同二氧化碳浓度和硝酸钾浓度对雨生红球藻生长及虾青素累积的影响。结果表明,较高浓度的CO2(600×10-6)能够显著促进雨生红球藻的生长、光合作用的进行和虾青素的累积。红球藻单个细胞内的虾青素含量随着培养液中硝酸钾浓度的降低而增加,绿色游动藻种和绿色不动藻种培养12 d后获得的最大虾青素值分别为10.93 pg/个和12.64 pg/个。连续通气是促进雨生红球藻生长及虾青素累积的一种有效碳源提供方式。 相似文献
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Gracilaria lemaneiformis Bory is an economically important alga that is primarily used for agar production. Although tetraspores are ideal seeds for
the cultivation of G. lemaneiformis, the most popular culture method is currently based on vegetative fragments, which is labor-intensive and time-consuming.
In this study, we optimized the conditions for tetraspore release and evaluated the photosynthetic activities of different
colonies formed from the branches of G. lemaneiformis using a PAM (pulse-amplitude-modulated) measuring system. The results showed that variations in temperature and salinityhad
significant effects on tetraspore yield. However, variations in the photon flux density (from 15 μmol m−2 s−1 to 480 μmol m−2 s−1) had no apparent effect on tetraspore yield. Moreover, the PAM-parameters Y(I), Y(II), ETR(I), ETR(II) and F
v/F
m of colonies formed from different branches showed the same trend: parameter values of first generation branches>second generation
branches>third generation branches. These results suggest that the photosynthetic activities of different colonies of branches
changed with the same trend. Furthermore, photosynthesis in G. lemaneiformis was found to be involved in vegetative reproduction and tetraspore formation. Finally, the first generation branches grew
slowly, but accumulated organic compounds to form large numbers of tetraspores. Taken together, these results showed that
the first generation branches are ideal materials for the release of tetraspores. 相似文献
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A harmful algae bloom (HAB) is a dense aggregation of algae in a marine or aquatic environment that can result in significant
environmental problems. To forecast the occurrence of HAB, development of a rapid and precise detection method is urgently
required. In this study, two Skeletonema costatum-like diatoms (SK-1 and SK-2), were identified morphologically under a light microscope, and detected using fluorescent in situ hybridization (FISH). Strain SK-1 was isolated from a frequently HAB affected area of the East China Sea, and strain SK-2
from an aquatic farm in Qingdao, China. Fluorescent DNA probes were designed that were complementary to the ITS sequence (including
5.8S rDNA) of strain SK-1. After hybridization, strong green fluorescence was observed in cells of strain SK-1 under an epifluorescence
microscope; however, no such fluorescence was observed with strain SK-2, which indicates that probes hybridized only the DNA
of the target strain, SK-1, in species-specific manner, and that the two strains do not belong to a same species. This finding
was confirmed by ITS sequence analysis. The FISH technique used in this study was sensitive, simple, and rapid, and is a promising
tool for detecting target HAB species in natural environments. 相似文献
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随着DNA序列测定技术的飞速发展，人们可以在很短的时间内获得某种生物基因组的全序列。生物体的生命活动主要依赖于基因组的产物——蛋白质组，但是蛋白质组的研究相对于基因组来说却要复杂得多。蛋白质组技术是在细胞内整体蛋白质水平上进行研究，从蛋白质整体活动的角度来研究生命活动的规律。综述了蛋白质组和蛋白质组学出现的背景、起源、概念、研究技术、研究内容、面对的挑战、开展该项研究的意义、陆生动植物和微生物蛋白质组学的研究进展及海洋生物蛋白质组学的研究概况。目前，普通生物方面的蛋白质组工作开展得较多，海洋生物方面却相对滞后。种种迹象表明，开展海洋生物蛋白质组学研究，对揭示生命的奥秘、促进海洋资源的开发都有重大的推动作用。 相似文献
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龙须菜琼胶的提取方法研究 总被引：2，自引：2，他引：2
采用多种方法提取了龙须菜(Gracilaria lemaneiformis)中的琼胶,并研究了碱处理、微波处理、超声波处理等方法对龙须菜的琼胶提取率、硫酸基质量分数和多糖质量分数的影响。结果表明:当碱质量分数由2.5%增加到10%时,琼胶产率由15%降低到9.8%,硫酸基质量分数由2.66%降低到2.06%,同时,多糖质量分数由98.3%增加到102.6%;微波和超声波处理在不同程度上增加了琼胶的提取率和多糖质量分数,同时降低了硫酸基质量分数。另外,还从宏观和微观两个角度考察了龙须菜碱处理前后藻体的变化。 相似文献