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5种有机磷农药对鮸状黄姑鱼胚胎和仔鱼毒性的研究   总被引:10,自引:0,他引:10  
研究了敌百虫、敌敌畏、甲胺磷、呋喃丹和乐果等5种有机磷农药对Miao状黄姑鱼(Nibea miichthiodides)胚胎和前期仔鱼的毒性。结果表明,在实验设置的浓度条件下,甲胺磷对胚胎的毒性最大;乐果对胚胎的毒性最小;敌百虫和敌敌畏对仔鱼的毒性大于其他3种有机磷农药;敌百虫、敌敌畏、甲胺磷和呋喃丹对仔鱼的毒性大于对胚胎的毒性,而乐果对两者的毒性是基本相似。  相似文献   
2.
2002年5~6月,在集美大学水产学院水产试验场,采用生态学方法对人工培育的双棘黄姑鱼(Nibeadiacanthus(Lacpde))仔、稚、幼鱼的摄食习性与生长特性进行分析。结果表明,在人工培育条件下,双刺黄姑鱼仔、稚、幼鱼的摄食率达93.1%,摄食量占体质量的4.09%~21.07%,日摄食量占体质量的23.67%~57.24%;摄食具明显的昼夜节律,黄昏为摄食高峰。摄食量与体质量的关系式为y=0.1267x0.7878,全长与日龄的关系式为L=1.8603e0.0723D,体质量与日龄的回归方程为M=0.0203e0.2484D。全长与体质量的回归方程为M=0.0024L3.4633。  相似文献   
3.
1996年3~6月采用人工注射LRH-A和HCG诱导状黄姑鱼自然产卵受精,观察6批次胚胎发育的结构特征和1~16日龄仔鱼形态变化及温度、盐度对状黄姑鱼早期发育和存活的影响,为状黄姑鱼大规模人工繁殖和苗种培育提供科学依据。根据其外部形态和胚胎行为的出现将状黄姑鱼胚胎发育划分为17期,3日龄开口;状黄姑鱼受精卵孵化的温度范围为16.3~30℃,最适孵化温度为22~24℃;孵化盐度范围为13.7~53,最适孵化盐度范围为26.9~40.7,其中盐度为7.2时受精卵无法孵化,盐度在40.7以上的受精卵全部为浮性,盐度为26.9以下全部呈沉性;状黄姑鱼仔鱼存活的适合盐度为20.2~33.4,随着仔鱼的生长,对耐盐的适应性也逐渐增强。  相似文献   
4.
Bacterin was prepared by formalin-inactivating the virulent strain of Vibrio hollisae isolated from diseased Seriola dumerili (amberjack) suffering from vibriosis. Healthy S. dumeriIi were vaccinated by respective procedures of intramuscular injection, immersion, and orally administration. Results of the three different vaccinations were compared. Blood was drawn from the vaccinated fish every 7 days, and the antibody titers and lysozyme activities of the sera were determined. The antibody titer of injected fish was 1:40 at 7 d, and reached its peak of 1:320 at 28 d, while the fish vaccinated by immersion and orally administration exhibited weak antibody responses, the antibody titres of 〈1:10, 1:20, 1:160 were observed at 7 d, 14 d, 35 d respectively. Compared with the control, the vaccinated fish exhibited significantly higher lysozyme activities (P〈0.05). Upon challenge with virulent strain, the relative percent survival (RPS) of injected, immersed and oral administrated fish were 75%, 45%, and 40% respectively, and the injected fish showed significantly higher RPS than immersed and oral administrated fish. The results suggested that vaccination of S. dumerili by the injection would be the best strategy to prevent the vibriosis in S. dumerili farm.  相似文献   
5.
从患病的杜氏(鱼师)肾脏分离出Y-1菌.人工感染试验,当注射9.0×107CFU/尾时,Y-1菌可致试验鱼100%死亡;注射9.0×106CFU/尾时,死亡率为50%,症状与自然发病相似,结果表明该分离菌为致病菌.Y-1菌为革兰氏阴性,有动力,杆状,极生单鞭毛;发酵葡萄糖,还原硝酸盐,氧化酶、触酶阳性,赖氨酸脱羧酶、鸟氨酸脱羧酶、精氨酸双水解酶阴性.根据形态及生理生化特性,Y-1菌应为弧菌Vibriosp..药敏试验结果表明,该菌对氨基糖甙类、呋喃类、喹诺酮类及大环内脂类药物敏感.  相似文献   
6.
报道杜氏鰤(Seriola dumerili)人工苗养殖生物学特性及养殖技术研究结果。在海区水温6-30.5℃。海水盐度14.33,溶解氧6.5-7.3mg/L,pH7.5-8.1,水流为15-35cm/s的网箱中,杜氏鰤人工苗叉长40-50mm,体质量1.5-1.7g,经208d养殖,叉长达到330-388mm,体质量达到950-1400g,饲料系数为11,成活率为71.7%;养殖720d,体质量达4.5-6.5k,饲料系数为8;养殖l080d,体质量达7.5-11.5kg,饲料系数为7;养殖l440d,体质量达13.,15.5k异,饲料系数为6;养殖l800d,体质量达15.5-18.5kg,饲料系数为5。第4年性腺发育成熟,4-5月份,海上网箱人工催产,平均每尾雌鱼可产卵1.8kg左右。  相似文献   
7.
Bacterin was prepared by formalin-inactivating the virulent strain of Vibrio hollisae isolated from diseased Seriola dumerili (amberjack) suffering from vibriosis. Healthy S. dumerili were vaccinated by respective procedures of intramuscular injection, immersion, and orally administration. Results of the three different vaccinations were compared. Blood was drawn from the vaccinated fish every 7 days, and the antibody titers and lysozyme activities of the sera were determined. The antibody titer of injected fish was 1:40 at 7 d, and reached its peak of 1:320 at 28 d, while the fish vaccinated by immersion and orally administration exhibited weak antibody responses, the antibody titres of <1:10, 1:20, 1:160 were observed at 7 d, 14 d, 35 d respectively. Compared with the control, the vaccinated fish exhibited significantly higher lysozyme activities (P<0.05). Upon challenge with virulent strain, the relative percent survival (RPS) of injected, immersed and oral administrated fish were 75%, 45%, and 40% respectively, and the injected fish showed significantly higher RPS than immersed and oral administrated fish. The results suggested that vaccination of S. dumerili by the injection would be the best strategy to prevent the vibriosis in S. dumerili farm.  相似文献   
8.
美国红鱼“腐皮病”病原的初步研究   总被引:7,自引:2,他引:7  
从有典型“腐皮病”症状的美国红鱼(Sciaenops ocellatus)中分离到一株优势菌。人工感染菌液浓度为10^8CFU/mL时,可致健康美国红鱼出现与自然发病相似的症状,且100%死亡,确认该分离菌为美国红鱼“腐皮病”的致病菌。该菌的主要生理生化特征为革兰氏染色阴性,0/129(150μg)敏感,TCBS上生长呈黄色,氧化酶阳性,伏-普二试验阴性,葡萄糖产酸不产气,蔗糖发酵阳性,甘露糖发酵阳性,阿拉伯糖发酵阳性,肌醇阴性,赖氨酸阴性,精氨酸阳性,鸟氨酸阴性,硝酸盐还原阳性,七叶灵水解阴性,ONPG阳性,有动力,在盐度0~60生长,80和100不生长,30~40℃生长,4℃、45℃不生长,鉴定该菌为河流弧菌(Vibrio fluvialis)。药敏试验表明,该菌对多粘菌素B、四环素、痢特灵敏感;对卡那霉素、链霉素、复方新诺明、呋喃妥咽、庆大霉素、菌必治不敏感;对氟霉素、青霉素、氨苄青霉素、氟哌酸、环丙沙星、利福平中度敏感。  相似文献   
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