Isolation of protein from Chlorella sorokiniana CY1 using liquid biphasic flotation assisted with sonication through sugaring-out effect     

Chia  Shir Reen  Chew  Kit Wayne  Show  Pau Loke  Sivakumar  Manickam  Ling  Tau Chuan  Tao  Yang 《中国海洋湖沼学报》2019,37(3):898-908

Microalgae, a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical, bioenergy and food applications. This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga, Chlorella sorokiniana CY1 which was assisted with sonication. A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides, glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein). The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases, as the yield of protein increased to 77%. The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well. The optimum protein productivity was obtained with 10 s of resting time in between sonication. Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode. The optimum condition for protein extraction were found as followed: 200 g/L glucose as bottom phase with volume ratio of I:1.25, 10 s of resting time for ultrasonication, 5 s of ultrasonication in pulse mode and 0.25 g of biomass weight. The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.  相似文献   

Fracture mechanics analysis of multiple cracks in anisotropic media     

Chia‐Huei Tu  C.‐S. Chen  T.‐T. Yu 《国际地质力学数值与分析法杂志》2011,35(11):1226-1242

This paper presents a single‐domain boundary element method (BEM) for linear elastic fracture mechanics analysis in the two‐dimensional anisotropic material. In this formulation, the displacement integral equation is collocated on the un‐cracked boundary only, and the traction integral equation is collocated on one side of the crack surface only. A special crack‐tip element was introduced to capture exactly the crack‐tip behavior. A computer program with the FORTRAN language has been developed to effectively calculate the stress intensity factors of an anisotropic material. This BEM program has been verified having a good accuracy with the previous researches. Furthermore, by analyzing the different anisotropic degree cracks in a finite plate, we found that the stress intensity factors of crack tips had apparent influence by the geometry forms of cracks and media with different anisotropic degrees. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献   

Coupled surface and ground water models for investigating hydrological processes     

Ray‐Shyan Wu  Dong‐Sin Shih  Ming‐Hsu Li  Chia‐Ching Niu 《水文研究》2008,22(8):1216-1229

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Nonlinear influences on ocean waves observed by X-band radar     

Li-Chung Wu  Dong-Jiing Doong  Beng-Chun Lee  Laurence Zsu-Hsin Chuang  Chia Chuen Kao 《Marine Geophysical Researches》2008,29(1):43-50

The purpose of this study is to discuss the influence of signal nonlinearity upon X-band radar observations. A method for estimating the degree of nonlinearity by bispectral analysis was applied and discussed. We found that bispectral analyses from spatial radar backscatter series are similar to results obtained from water level time series. In addition, the average nonlinear degree from radar backscatter is related to wind speed. The accuracy of wave observations derived by consideration of the nonlinear effect from radar backscatter was also investigated. The estimated error in wave height from the radar data is also related to the degree of nonlinearity. In order to improve accuracy, the modulation transfer function method was applied in order to eliminate the influence of nonlinearity.  相似文献   

Evaluating the stress intensity factors of anisotropic bimaterials using boundary element method     

Chao‐Shi Chen  Chien‐Chung Ke  Chia‐Huei Tu 《国际地质力学数值与分析法杂志》2008,32(11):1341-1364

This paper presents a boundary element method (BEM) procedure for a linear elastic fracture mechanics analysis in two‐dimensional anisotropic bimaterials. In this formulation, a displacement integral equation is only collocated on the uncracked boundary, and a traction integral equation is only collocated on one side of the crack surface. A fundamental solution (Green's function) for anisotropic bimaterials is also derived and implemented into the boundary integral formulation so that except for the interfacial crack part, the discretization along the interface can be avoided. A special crack‐tip element is introduced to capture the exact crack‐tip behavior. A computer program using FORTRAN has been developed to effectively calculate the stress intensity factors of an anisotropic bimaterial. This BEM program has been verified to have a good accuracy with previous studies. In addition, a central cracked bimaterial Brazilian specimen constituting cement and gypsum is prepared to conduct the Brazilian test under diametral loading. The result shows that the numerical analysis can predict relatively well the direction of crack initiation and the path of crack propagation. Copyright © 2007 John Wiley & Sons, Ltd.  相似文献   

On the mass-radius relation in close binary systems     

T. T. Chia 《Astrophysics and Space Science》1977,51(1):33-38

In a close binary system with circular orbits in which mass transfer occurs when material from one star fills up and possibly spills over its Roche lobe, the usual mass-radius relation cannot be used as it would lead to unreasonable results in some situations.  相似文献   

THE STRATEGIC STRAIT WITH SPECIAL REFERENCE TO THE MALACCA STRAITS1     

Allan Chia  Lee Yong Leng 《Singapore journal of tropical geography》1987,8(2):97-113

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Changes in hydrogeological properties of the River Choushui alluvial fan aquifer due to the 1999 Chi-Chi earthquake, Taiwan     

Cheng-Shin Jang  Chen-Wuing Liu  Yeeping Chia  Li-Hsin Cheng  You-Ching Chen 《Hydrogeology Journal》2008,16(2):389-397

Changes in hydrogeological properties of the River Choushui alluvial fan aquifer before and after the 1999 Chi-Chi earthquake, Taiwan, have been identified using pumping tests. Three wells, SH2, YL2 and SC2, located in a compressional zone with high coseismic groundwater levels, were tested. The threshold of the aquifer deformation with respect to transmissivity (T) is greater than that with respect to storage coefficient (S). Decreases in the post-earthquake S are approximately 60% at SH2 and SC2, indicating aquifer compression after the Chi-Chi earthquake. Changes in the post-earthquake T range from 61% increase to 0.8% decrease. Moreover, results from anisotropy analysis of T at SC2 further illustrate that normal stresses induced by the Chi-Chi earthquake have consolidated soil particles. Soil particles dilated laterally after the earthquake, resulting in an increase of the equivalent T. The changes in hydrogeological properties have a considerable influence on spatiotemporal fluid pressure and horizontal groundwater movement, resulting in different amounts of drawdown during post-earthquake pumping.  相似文献   

The effects of gravitational radiation on circular binary systems with spins     

T. T. Chia 《Astrophysics and Space Science》1985,114(1):151-156

In any theory of gravitation that predicts the existence of gravitational waves, it is shown that, in the absence of mass exchange and mass loss and subjected to a condition, a circular binary system with spherically-symmetrical components cannot evolve in such a way that the spin angular velocity is always a linear function of the orbital velocity. Probably this relation between the angular velocities cannot be realized in any time interval. In particular, the system cannot remain in a synchronized state. Of eight special cases of evolution of this binary system, three cases, where the separation increases, are shown to be kinematically impossible while a restriction on the variation of a parameter governing the evaluation of the system can be made in another. In one of the kinematically allowed special cases the separation increases.  相似文献   

Equivalence of some integrals of the radiation theory     

T. T. Chia 《Astrophysics and Space Science》1977,46(1):239-246

A definite integral which occurs in radiation theory is shown to be equal in value to another definite integral by evaluating the flux from a spherically symmetrical radiating sphere in two ways. As a corollary, an alternate proof of the invariance of the specific intensity of a ray in empty space along its path is presented.Furthermore, the equality of these two indefinite integrals leads to the conversion of members of a class of indefinite and definite integrals involving arbitrary functions of angle into other integrals. These transformations facilitate the calculation of some of these integrals which arise not only in the theory of radiation, but in other physical situations with spherical or axial symmetry — especially those in which inverse-square laws are involved.  相似文献