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1.
The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological
specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine
bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed
by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent
microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration
and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were
flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based
on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is
easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement
of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as
a new tool to study the fine structures of marine bacteria.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
2.
The R/V Mirai conducted hydrographic surveys in the western Arctic Ocean during summer 2004 (Mirai04) over wide east-west
ranges from Alaska to eastern Siberia, where sea-ice cover has been greatly reduced in recent summers. The obtained data reveal
differences in silicate profiles between shelf slope areas east and west of the Chukchi Plateau, the ridge that divides the
Canada Basin into the Alaskan and east Siberian sides. East of the plateau, a single silicate maximum was found in a layer
of Pacific-origin winter water, as examined in many previous studies. In contrast, west of the plateau, we found vertical
double silicate maxima, which are reported for the first time in this study. The shallower silicate maximum corresponded to
an N** minimum, signaling denitrification at the shelf bottom. This suggests that the shallower silicate maximum was caused
by the spreading of shelf water. In contrast, the deeper silicate maximum corresponded to an oxygen minimum and a maximum
silicate/phosphate ratio (Si/P), suggesting that this deeper maximum resulted from the decomposition of opal-shelled organisms.
We also compared a silicate profile from Mirai04 to aprofile from the Arctic Ocean Section 94 (AOS94) expedition of 1994,
a heavy ice year. The results suggest that sea-ice loss has enhanced biological activities, likely resulting in the appearance
of the deeper silicate maximum. 相似文献
3.
Yoshida A Nomura H Toyoda K Nishino T Seo Y Yamada M Nishimura M Wada M Okamoto K Shibata A Takada H Kogure K Ohwada K 《Marine pollution bulletin》2006,52(1):89-95
Microbial responses to the addition of oil with or without a chemical dispersant were examined in mesocosm and microcosm experiments by using denaturing gradient gel electrophoresis of bacterial ribosomal DNA and direct cell counting. When a water-soluble fraction of oil was added to seawater, increases in cell density were observed in the first 24h, followed by a decrease in abundance and a change in bacterial species composition. After addition of an oil-dispersant mixture, increases in cell density and changes in community structure coincided, and the amount of bacteria remained high. These phenomena also occurred in response to addition of only dispersant. Our results suggest that the chemical dispersant may be used as a nutrient source by some bacterial groups and may directly or indirectly prevent the growth of other bacterial groups. 相似文献
4.
5.
GIS-based weights-of-evidence modelling of rainfall-induced landslides in small catchments for landslide susceptibility mapping 总被引：10，自引：0，他引：10
Ranjan Kumar Dahal Shuichi Hasegawa Atsuko Nonomura Minoru Yamanaka Takuro Masuda Katsuhiro Nishino 《Environmental Geology》2008,54(2):311-324
Landslide susceptibility mapping is a vital tool for disaster management and planning development activities in mountainous terrains of tropical and subtropical environments. In this paper, the weights-of-evidence modelling was applied, within a geographical information system (GIS), to derive landslide susceptibility map of two small catchments of Shikoku, Japan. The objective of this paper is to evaluate the importance of weights-of-evidence modelling in the generation of landslide susceptibility maps in relatively small catchments having an area less than 4 sq km. For the study area in Moriyuki and Monnyu catchments, northeast Shikoku Island in west Japan, a data set was generated at scale 1:5,000. Relevant thematic maps representing various factors (e.g. slope, aspect, relief, flow accumulation, soil depth, soil type, land use and distance to road) that are related to landslide activity were generated using field data and GIS techniques. Both catchments have homogeneous geology and only consist of Cretaceous granitic rock. Thus, bedrock geology was not considered in data layering during GIS analysis. Success rates were also estimated to evaluate the accuracy of landslide susceptibility maps and the weights-of-evidence modelling was found useful in landslide susceptibility mapping of small catchments. 相似文献
6.
Principal fault zone width and permeability of the active Neodani fault, Nobi fault system, Southwest Japan 总被引：2，自引：0，他引：2
A. Tsutsumi S. Nishino K. Mizoguchi T. Hirose S. Uehara K. Sato W. Tanikawa T. Shimamoto 《Tectonophysics》2004,379(1-4):93-108
The internal structure and permeability of the Neodani fault, which was last activated at the time of the 1891 Nobi earthquake (M8.0), were examined through field survey and experiments. A new exposure of the fault at a road construction site reveals a highly localized feature of the past fault deformation within a narrow fault core zone. The fault of the area consists of three zone units towards the fault core: (a) protolith rocks; (b) 15 to 30 m of fault breccia, and (c) 200 mm green to black fault gouge. Within the fault breccia zone, cataclastic foliation oblique to the fault has developed in a fine-grained 2-m-wide zone adjacent to the fault. Foliation is defined by subparallel alignment of intact lozenge shaped clasts, or by elongated aggregates of fine-grained chert fragments. The mean angle of 20°, between the foliation and the fault plane suggests that the foliated breccia accommodated a shear strain of γ<5 assuming simple shear for the rotation of the cataclastic foliation. Previous trench surveys have revealed that the fault has undergone at least 70 m of fault displacement within the last 20,000 years in this locality. The observed fault geometry suggests that past fault displacements have been localized into the 200-mm-wide gouge zone. Gas permeability analysis of the gouges gives low values of the order of 10−20 m2. Water permeability as low as 10−20 m2 is therefore expected for the fault gouge zone, which is two orders of magnitude lower than the critical permeability suggested for a fault to cause thermal pressurization during a fault slip. 相似文献
7.
A newly developed direction finding (DF) technique for auroral hiss based on the measurement of time differences of wave arrival was carried out in 1978 at Syowa Station (geomag. lat. -70.4°), Antarctica and its two slave unmanned observing points located at about 20 km distances from Syowa. The auroral hiss signals (0.3–100 kHz) received at the two spaced points were transmitted to Syowa by a wide-band telemeter of 2 GHz. The arrival time difference of auroral hiss between Syowa and each spaced point was automatically determined by cross-correlating the waveforms of the received signals, and then the incident and azimuthal angles were measured with an accuracy of about 10°.It has been found that the new DF technique can determine localized exit regions at the ionospheric level which show rapid temporal movements. A comparison of the DF results with ground-based auroral data has shown that impulsive type auroral hiss with a wide-band frequency range has not emerged from the whole region of a bright aurora but from some localized regions of bright electron auroras at the ionospheric level, and that the arrival directions of auroral hiss change rapidly in accordance with the auroral movements. 相似文献
8.
Ana M. Aguilar-Islas Robert Rember Shigeto Nishino Takashi Kikuchi Motoyo Itoh 《Polar Science》2013,7(2):82-99
The concentration of dissolved iron (DFe) and suspended leachable particulate iron (LPFe) in the water column of the western Beaufort Sea were investigated during the late summer of 2010. Elevated concentrations of surface DFe (0.49–1.42 nM) were similar to those reported in resent studies, likely reflecting input from melting sea ice and river discharge. The rapid decrease in DFe (5.20–0.48 nM) and LPFe (88.2–1.83 nM) values observed from inshore to offshore in Pacific influenced waters, suggest scavenging processes limit the input of DFe from the shelf to the deep basin. However, frequent eddies found in this region are likely important in promoting lateral advection, as suggested by higher surface DFe concentrations at an offshore station in the vicinity of a warm-core eddy. Within the Atlantic layer, relatively homogeneous DFe (0.69–0.80 nM) and LPFe (1.18–2.13 nM) concentrations were observed at all the stations, reflecting a balance in the interplay between input and removal processes within this watermass. An input of DFe east of the Lomonosov Ridge was inferred by comparing DFe values within the core of Atlantic water between the Eastern and Western Arctic. 相似文献
9.
Toshiyuki Ohtsuka Mitsuru Hirota Xianzhou Zhang Ayako Shimono Yukiko Senga Minguan Du Seiichiro Yonemura Shigeto Kawashima Yanhong Tang 《Polar Science》2008,2(4):277-285
To accurately estimate soil organic carbon (SOC) storage in upper alpine to nival zones on the Tibetan Plateau, we inventoried SOC pools in 0–0.3 m profiles along an altitudinal gradient (4400–5300 m asl). We also studied vegetation properties and decomposition activity along the gradient to provide insight into the mechanisms of SOC storage. The vegetation cover and belowground root biomass showed a gradual increased with altitude, reaching a peak in the upper alpine zone at 4800–4950 m before decreasing in the nival zone at 5200–5300 m.Decomposition activity was invariant along the altitudinal gradient except in the nival zone. SOC pools at lower sites were relatively small (2.6 kg C m−2 at 4400 m), but increased sharply with altitude, reaching a peak in the upper alpine zone (4950 m; 13.7 kg C m−2) before decreasing (1.0 kg C m−2 at 5300 m) with altitude in the nival zone. SOC pool varied greatly within individual alpine meadows by a factor of five or more, as did bulk density, partly due to the effect of grazing. Inventory data for both carbon density and bulk density along altitudinal gradients in alpine ecosystems are of crucial importance in estimating global tundra SOC storage. 相似文献
10.
M. Tahara K. Uehira H. Shimizu M. Nakada T. Yamada K. Mochizuki M. Shinohara M. Nishino R. Hino H. Yakiwara H. Miyamachi K. Umakoshi M. Goda N. Matsuwo T. Kanazawa 《Physics of the Earth and Planetary Interiors》2008,167(1-2):19-33
The Hyuganada region, a forearc region of Southwest Japan, is characterized by several interesting geological and geophysical features, i.e., significant aseismic crustal uplift of 120 m during the past 120 thousand years at the Miyazaki Plain, negative free-air gravity anomalies with the maximum magnitude of −130 mgal, and relatively less cohesive interplate coupling compared with that for off the Shikoku and Kii Peninsula. In order to examine the causes of these observations, we determined a detailed three-dimensional seismic velocity structure based on the seismic data observed by ocean bottom seismometers (OBS) and land stations. P- and S-wave tomographic velocity structures clearly indicate the subducting slab and also the zones of high Poisson's ratio at 25–35 km depth along the coastline of the northeastern part of the Hyuganada. The region with high Poisson's ratio may correspond to the serpentinized mantle wedge as suggested for other mantle wedges, and appears to be coincident with the zone for observed aseismic slips such as the slow-slip and after-slip events. Also, the detection may be related to a relatively weak interplate coupling in the Hyuganada region. The tomographic structures also indicate low velocity zones with a horizontal scale comparable to the Kyushu-Palau Ridge in and around the subducting slab. If we assume that the low velocity zones correspond to the subducted Kyushu-Palau Ridge, then the predicted gravity anomaly due to the density contrast between the low velocity zones and the surrounding region can explain about 60% of the gravity anomaly in the Hyuganada region. The buoyancy is probably an important factor for the crustal uplift observed in the Miyazaki Plain, the steep bending of the subducting slab and the normal fault-type earthquakes around the Hyuganada region. 相似文献