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1.
The atomic force microscopy (AFM) is a recently developed, bench-top instrument that can image the surface structures of biological
specimens at high resolution with simultaneous measurement of their size. This paper describes the application of AFM to marine
bacteria. Both natural and cultured bacteria were retained on a filter or placed on glass, washed, air-dried and observed
by AFM. The instrumental condition, the choice of suitable filter, effect of fixation and filtration, comparison with epifluorescent
microscopic (EFM) count, and the size and shape of bacterial cells were investigated. An Isopore filter was best for concentration
and subsequent observation because of its surface flatness. Cross section images showed that both rod and coccoid cells were
flattened, the former usually having a two-humped shape. Bacterial cells were differentiated from non-living particles based
on their cross section shape and size. Bacterial counts by AFM and EFM showed good agreement. Although size measurement is
easily done by the instrument, AFM tends to overestimate the size of microspheres. More work is thus needed on the size measurement
of living organisms. Because AFM easily provides images of natural bacterial cells at high magnification, it can be used as
a new tool to study the fine structures of marine bacteria.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
2.
HAN Jian LI Guoxiang Kubota Shin Ou Qiang Toshino Sho Wang Xing Yang Xiaoguang Uesugi Kentaro Masato Hoshino Sasaki Osamu Kano Harumas Sato Tomohiko Komiya Tsuyoshi 《《地质学报》英文版》2016,90(1):38-65
The early Cambrian pentamerous microfossil Olivooides/Punctatus in South China, which is characterized by a diagnostic stellate tubular apex, has been well-known for its almost complete development sequence that can be confidently traced from embryos and hatched juveniles, to conical adults. However, its zoological affinity remains highly controversial. Here we describe the internal microanatomic structures of the soft-body inside the peridermal theca of Olivooides multisulcatus Qian, 1977, including interradial pairs of tentacles, adradial and perradial frenula, perradial oral marginal lappets and twins of perradial gastric saccule-like humps as well as a circular velarium with striated coronal muscles. Particularly, one specimen shows bifurcated velarial canals along the bell aperture. Both the components of the soft-body and the external theca (or cyst) are arranged in perfect pentaradial symmetry. These characteristics are more compatible with those of living cubomedusans and co-occurring Cambrian athecate embryonic cubozoans. Concerning the presence of peridermal theca, Olivooides most likely represents an extinct thecate stem-group cubomedusae but devoid of both perradial eyes and specialized pedalia. The well-grown soft body inside the peridermal tube displays a set of mixed features of both polyp and medusa. 相似文献
3.
Malarvannan G Takahashi S Isobe T Kunisue T Sudaryanto A Miyagi T Nakamura M Yasumura S Tanabe S 《Marine pollution bulletin》2011,63(5-12):172-178
Three species of sea turtles (green, hawksbill and loggerhead turtles) stranded along the coasts or caught (by-catch) around Ishigaki Island and Kochi, Japan were collected between 1998 and 2006 and analyzed for six organohalogen compounds viz., PBDEs, PCBs, DDTs, CHLs, HCHs and HCB. The present study is the first and foremost to report the occurrence of organohalogen compounds in the sea turtles from Japan. Among the compounds analyzed, concentrations of PCBs, DDTs and CHLs were the highest in all the turtle samples. PBDEs were ubiquitously present in all the turtle species. Comparing with the other two species, concentrations of organohalogens in green turtle were relatively low and decreasing trend in the concentrations were noted with increasing carapace length. Concentrations of OCs in sea turtles from the coasts of Ishigaki Island and Kochi were relatively low as compared to those from other locations in the world. 相似文献
4.
Yoshida A Nomura H Toyoda K Nishino T Seo Y Yamada M Nishimura M Wada M Okamoto K Shibata A Takada H Kogure K Ohwada K 《Marine pollution bulletin》2006,52(1):89-95
Microbial responses to the addition of oil with or without a chemical dispersant were examined in mesocosm and microcosm experiments by using denaturing gradient gel electrophoresis of bacterial ribosomal DNA and direct cell counting. When a water-soluble fraction of oil was added to seawater, increases in cell density were observed in the first 24h, followed by a decrease in abundance and a change in bacterial species composition. After addition of an oil-dispersant mixture, increases in cell density and changes in community structure coincided, and the amount of bacteria remained high. These phenomena also occurred in response to addition of only dispersant. Our results suggest that the chemical dispersant may be used as a nutrient source by some bacterial groups and may directly or indirectly prevent the growth of other bacterial groups. 相似文献
5.
Naoki Niwa Takuji Kobori Motoichi Takahashi Tomohiko Hatada Haruhiko Kurino Jun Tagami 《地震工程与结构动力学》1995,24(5):655-671
This paper describes a feasibility study on a high-damping device (HiDAM) installed in a building structure by way of a bracing mechanism. A seismic response analysis with respect to a high-rise building approximately 100 m high is reported. Proper adjustment of the damping coefficient of the HiDAM (oil damper) provided an over all damping factor of about 10–20 per cent to the building structure, and reductions in the response, deformation and shear forces were verified analytically. This paper reports also the performance confirmation tests conducted on a model device of the HiDAM which satisfied the specifications determined from the analytical results. The test results demonstrate the feasibility of the HiDAM and the possibility of utilizing it in high-rise buildings. 相似文献
6.
Xiong Yan Kanako Muramatsu Masahiro Hirata Kazato Oishi Ichirow Kaihotsu Tamio Takamura 《地球空间信息科学学报》2013,16(2):117-122
We plan to estimate global net primary production (NPP) of vegetation using the Advanced Earth Observing Satellite-II (ADEOS-II) Global Imager (GLI) multi-spectral data. We derive an NPP estimation algorithm from ground measurement data on temperate plants in Japan. By the algorithm, we estimate NPP using a vegetation index based on pattern decomposition (VIPD) for the Mongolian Plateau. The VIPD is derived from Landsat ETM+multi-spectral data, and the resulting NPP estimation is compared with ground data measured in a semi-arid area of Mongolia. The NPP estimation derived from satellite remote sensing data agrees with the ground measurement data within the error range of 15% when all above-ground vegetation NPP is calculated for different vegetation classifications. 相似文献
7.
Shin Takahashi Tomoko Oshihoi Tomohiko Isobe Tsunemi Kubodera 《Marine pollution bulletin》2010,60(2):187-196
Twelve species of deep-sea fishes collected in 2005 from the western North Pacific, off-Tohoku, Japan were analyzed for organohalogen compounds. Among the compounds analyzed, concentrations of DDTs and PCBs (up to 23,000 and 12,400 ng/g lipid wt, respectively) were the highest. The present study is the foremost to report the occurrence of brominated flame retardants such as PBDEs and HBCDs in deep-sea organisms from the North Pacific region. Significant positive correlations found between δ15N (‰) and PCBs, DDTs and PBDEs suggest the high biomagnification potential of these contaminants in food web. The large variation in δ13C (‰) values observed between the species indicate multiple sources of carbon in the food web and specific accumulation of hydrophobic organohalogen compounds in benthic dwelling carnivore species like snubnosed eel. The results obtained in this study highlight the usefulness of deep-sea fishes as sentinel species to monitor the deep-sea environment. 相似文献
8.
Hikaru Sawada Ernest Tafumanei Mugandani Tomohiko Sato Yusuke Sawaki Shuhei Sakata Yukio Isozaki Shigenori Maruyama 《地学学报》2019,31(5):438-444
The ca. 2.2–2.1 Ga Magondi Supergroup on the Zimbabwe Craton in Southern Africa is mainly composed of sedimentary rocks deposited in a rift basin/passive continental margin, which record a unique episode in carbon isotope perturbation called the Lomagundi–Jatuli Event (LJE). This study reports new U–Pb ages of detrital zircons from the Deweras and Lomagundi groups of the Magondi Supergroup, and of igneous zircons from underlying granitoids, to constrain the timing of the LJE and to identify the provenance of the Magondi Supergroup. Most analysed detrital zircon grains range in ages between ca. 2.9 and 2.6 Ga. Three ca. 2.3–2.2 Ga detrital zircons from sandstone of the Deweras Group, with the youngest 207Pb‐206Pb age of 2,216 ± 22 Ma, indicate the onset of LJE in the Zimbabwe Craton was almost simultaneous to that in Fennoscandia and the Superior Craton, supporting the global synchronicity of the LJE. 相似文献
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10.
Muhammad Ilyas Agus Sudaryanto Iwan Eka Setiawan Tomohiko Isobe Shin Takahashi 《Marine pollution bulletin》2011,62(1):89-98
A total of 33 surficial sediments from riverine and coastal waters from Surabaya, Indonesia were analyzed for PCBs and BFRs. Concentrations of PCBs (62 congeners), PBDEs (14 congeners) and HBCDs (3 isomers) varied from <DL-420, <DL-35 and <DL-5.4 ng g−1 dw, respectively. Higher concentrations of these compounds were found in riverine than coastal sediments. Their levels and distribution were influenced by proximity to the point sources and TOC. The predominant congeners were CB-153, -28, -138, -149, -180, -33 and BDE-209, -207, -206, -197, -196, -183, -99, -47 for PCBs and PBDEs, respectively, and γ-isomer for HBCDs. Debromination of BDE-209 might be taking place producing lower toxic congeners in sediment. Levels of PCBs in riverine sediments were comparable with some polluted areas worldwide, but PBDEs and HBCDs were lower. Hazard assessment of PCBs indicated possible toxic potential, particularly in areas close to point sources. 相似文献