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硒结合蛋白1(selenium-binding protein 1,SBP1)是一种高度保守的蛋白,广泛参与了机体的多个理化过程。本研究首次获得了杂色鲍(Haliotis diversicolor)SBP1基因的全长c DNA序列2269 bp,并命名为Hd SBP1,ORF为1494 bp,共编码497个氨基酸。荧光定量PCR结果显示,Hd SBP1基因在杂色鲍各组织中均有表达,其中在鳃和肾中的表达量相对较高。缺氧处理后,Hd SBP1在鳃组织和血细胞中的表达量分别在处理24 h和192 h后显著升高(P0.05);高温应激之后,在鳃组织中,Hd SBP1的表达量在第1时相与第3时相显著升高(P0.05),而在血细胞中只有在第3时相表现出显著性差异;缺氧和高温联合应激之后,鳃组织中Hd SBP1的表达量在192 h显著上调(P0.05),在血细胞中Hd SBP1的表达量在0 h、4 h和24 h时均显著升高(P0.05);副溶血弧菌注射感染之后,在鳃组织中Hd SBP1的表达量在6 h和24 h时显著性上调(P0.05),而血细胞中Hd SBP1基因的表达量在每个时相的实验组都显著高于对照组(P0.05)。以上不同的应激条件均会导致Hd SBP1在不同组织中的表达量的显著变化,说明Hd SBP1在杂色鲍的免疫反应中可能扮演着重要的角色。 相似文献
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甲壳动物高血糖激素家族在调控甲壳动物的变态发育、生殖、蜕皮、血糖平衡及体内的渗透压平衡等方面起着重要的作用,甲壳动物高糖激素(CHH)是甲壳动物高血糖激素家族中的重要成员.通过Tail-PCR方法克隆获得拟穴青蟹(Scylla paramamosain)chh基因的近端启动子区序列,从翻译起始位点(ATG)起,共657 bp.生物信息学分析结果显示,转录起始位点位于翻译起始位点(ATG)上游第74个碱基(A),在转录起始位点(A)上游31 bp处存在TATA box;核心启动子区位于-41~+9 bp之间,包含在线软件预测的转录起始位点;潜在的转录因子结合位点包括Oct-1、C/EBPalp、GATA-1、Sp1、Ap-1、NF-κappa B、NF-1、RAP1、HNF-3等;目前获得的chh基因启动子区中没有Cp G岛.成功构建了chh基因4个启动子不同长度片段C1—C4,分别位于-583~+205、-388~+205、-286~+205、-46~+205 bp.含启动子不同长度片段的表达载体瞬时转染HEK293T细胞和Sf9细胞后的活性分析表明,在-286~-46、-583~-388 bp区域可能存在负调控基因表达的转录因子结合位点,在-388~-286 bp区域可能存在正调控基因表达的转录因子结合位点. 相似文献
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We investigated the effect of tributyltin (TBT) exposure on the concentration of malondialdehyde (MDA) and the activity levels
of the superoxide dismutase (SOD), catalase (CAT), and acid and alkaline phosphatase (ACP and AKP) enzymes in the small abalone,
Haliotis diversicolor supertexta. We collected samples of the hepatopancreas and hemolymph 2, 6, 24, 48, 96, and 192 h after exposure to 0.35 μg (Sn)/L TBT.
In the hepatopancreas, ACP activity was significantly higher in animals exposed to TBT 2, 24, and 96 h post-exposure compared
with the control animals. AKP activity was also higher after 2 h, but SOD and CAT activity was unchanged. The concentration
of MDA in the hemolymph was significantly higher than the control animals 2 and 6 h post-exposure. In the hemolymph of animals
exposed to TBT, ACP activity was significantly lower than in the control animals 192 h post-exposure, whereas AKP activity
was significantly lower 2 and 192 h post-exposure. Hemolymph SOD activity and levels of MDA were significantly lower than
in the control animals 24 h after exposure but significantly higher after 96 h. Our results demonstrate that exposure to TBT
cause rapid changes in ACP and AKP activity as well as altering the concentration of MDA in the hepatopancreas and hemolymph.
SOD and CAT do not appear to be involved in the detoxification of TBT in the hepatopancreas of small abalone. 相似文献
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