Cloning of a chub metallothionein cDNA and development of competitive RT-PCR of chub metallothionein mRNA as a potential biomarker of heavy metal exposure   总被引：3，自引：0，他引：3  

Hayes RA  Regondi S  Winter MJ  Butler PJ  Agradi E  Taylor EW  Kevin Chipman J 《Marine environmental research》2004,58(2-5):665-669

Metallothionein has been assayed in a range of aquatic animal tissues as an indicator of metal exposure. We sequenced chub (Leuciscus cephalus) metallothionein cDNA which showed over 90% homology to common carp, goldfish and stone loach and 77% homology to rainbow trout sequences for metallothionein. We then used the extended primer method to develop an accurate quantitative competitive RT-PCR assay for metallothionein mRNA. RT-PCR was used to measure metallothionein mRNA in feral chub from a range of field sites, with different levels of heavy metal pollution, in the West Midlands, UK. Measurements were complemented by analysis of liver and gill metallothionein protein by capillary electrophoresis. There was no significant difference in the metallothionein protein levels between fish of different rivers and there was no evidence of elevation of mRNA at the sites of highest metal exposure. The level of metal exposure (e.g. zinc, nickel and cadmium each ranging between 15 and 28 microg/l ) at the pH (7.5-8.5) of these rivers appears insufficient to elevate hepatic or gill metallothionein in chub. A lack of elevation of hepatic metallothionein mRNA in chub exposed to zinc, copper and manganese for 24 h and 10 days in the laboratory also suggests a non-responsiveness of this species.  相似文献   

Polycyclic aromatic hydrocarbons in water, seston and copepods in a harbour area in the Western Mediterranean (Ligurian Sea)   总被引：2，自引：0，他引：2  

Luigi Pane  Simona Boccardo  Fabio Bonfiglioli  Gian Luigi Mariottini  Francesca Priano  & Osvaldo Conio 《Marine Ecology》2005,26(2):89-99

Polycyclic aromatic hydrocarbons (PAHs) are widespread carcinogenic, mutagenic and teratogenic pollutants that can significantly affect ecosystems and exert an influence on marine planktonic and benthic communities as well as on higher organisms such as fish and mammals, including humans. In this paper the concentrations of six PAHs in water, seston and copepods were examined in a harbour zone of the Genova Gulf (Ligurian Sea) from November 1997 to June 1998. The importance of freshwater inputs coming from a polluted stream and the influence of seasonal and atmospheric factors were also considered. Highest PAH values were recorded during spring. Fluoranthene, Benzo[b]fluoranthene and Benzo[k]fluoranthene were the most abundant PAHs either in water, seston or copepods. The concentration of total PAHs in seawater was often lower than the detection limit: recorded values ranged between 0.001 and 0.06 ppb. In seston, total PAHs ranged between 0.033 and 6.26 ng·g^?1 and in copepods between 0.01 and 2.34 ng·g^?1. With this study, PAH contamination in the harbour zone of Genova Sestri‐Ponente and the importance of inputs of organic pollutants coming from land and freshwaters have been emphasized.  相似文献   

Plio-Quaternary continental deposits of the Castellamonte area, between Orco and Dora Baltea Basins (Torino Province, Italy)     

Maria Gabriella Forno  Simona Ferrando 《Quaternary International》2008,190(1):103

New geologic mapping of the area near Castellamonte (Torino Province) describes a wide Plio-Quaternary continental succession, up to about 100-m thick. This sequence is distinguished according to allostratigraphic criteria. Altitude, degree of pedogenesis and sediment supply from different basins are also used. Within a single alloformation, several lithofacies are described.Fine-grained fan-delta sediments (Vespia Alloformation) are described in the lower part of the continental succession. The fossil plant remains indicate an Early–Middle Pliocene age. These sediments show an evident deformation and fill ancient lacustrine basins, likely associated with the Canavese Line trend. Their wide distribution and considerable thickness suggest subsidence.Coarse-grained glacial, outwash and fluvial sediments (Colleretto Castelnuovo, S. Defendente, Case Musso, Pagliero, Serra and Piverone Alloformations) are described in the upper part of the continental succession. They consist of undeformed sediments deposited during Middle to Late Pleistocene. These sediments indicate the presence of small local glaciers along the current valley trends. The terracing ratios among the different units and their small thicknesses indicate subsequent differential uplift.  相似文献   

The influence of physical and biological processes on the ichthyoplankton communities in the Gulf of Sirte (Southern Mediterranean Sea)          下载免费PDF全文

Angela Cuttitta  Sergio Bonomo  Salem Zgozi  Angelo Bonanno  Bernardo Patti  Enza Maria Quinci  Marco Torri  Mohamed Hamza  Abdul Fatah  Daw Haddoud  Akram El Turki  Abdul Bari Ramadan  Simona Genovese  Salvatore Mazzola 《Marine Ecology》2016,37(4):831-844

This paper reports on the first study on the ichthyoplankton community structure in the Gulf of Sirte and its spatial distribution in relation to environmental conditions and zooplankton abundance. The results make an important contribution to the future management of fisheries in this unexplored, but much exploited, area. Biological samples were collected in July 2008 using a Bongo40 net. In total, 1914 larvae were found and 1652 of these were identified. In particular, bathypelagic taxa were the most abundant, followed by demersal, mesopelagic, pelagic and epipelagic taxa. The ichthyoplankton community had a patchwork distribution influenced by oligotrophic conditions, the bottom depth and oceanographical features. The results suggest that environmental forcings were able to transport the ichthyoplankton to productive areas. Indeed, maximum fish egg densities were found in coastal stations in correspondence with the Atlantic Tunisian Current inflow, whereas larvae were mainly concentrated in the east side of the Gulf, probably as a result of advection by the anticyclonic circulation. Additionally, the distribution patterns of the total larvae density and the different assemblages were well matched with the abundance of the zooplankton, probably determining final larval survival, growth and recruitment.  相似文献   

Climate and human land-use as a driver of Lake Narlay (Eastern France,Jura Mountains) evolution over the last 1200 years: implication for methane cycle     

Simon Belle  Valérie Verneaux  Laurent Millet  David Etienne  Andrea Lami  Simona Musazzi  Jean-Louis Reyss  Michel Magny 《Journal of Paleolimnology》2016,55(1):83-96

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Impact of drilling mud on chemistry and microbiology of an Upper Triassic groundwater after drilling and testing an exploration well for aquifer thermal energy storage in Berlin (Germany)     

Simona Regenspurg  Mashal Alawi  Guido Blöcher  Maria Börger  Stefan Kranz  Ben Norden  Ali Saadat  Traugott Scheytt  Lioba Virchow  Andrea Vieth-Hillebrand 《Environmental Earth Sciences》2018,77(13):516

After completion of an exploration well, sandstones of the Exter Formation were hydraulically tested to determine the hydraulic properties and to evaluate chemical and microbial processes caused by drilling and water production. The aim was to determine the suitability of the formation as a reservoir for aquifer thermal energy storage. The tests revealed a hydraulic conductivity of 1–2 E-5 m/s of the reservoir, resulting in a productivity index of 0.6–1 m³/h/bar. A hydraulic connection of the Exter Formation to the overlaying, artesian “Rupelbasissand” cannot be excluded. Water samples were collected for chemical and microbiological analyses. The water was similarly composed as sea water with a maximum salinity of 24.9 g/L, dominated by NaCl (15.6 g/L Cl and 7.8 g/L Na). Until the end of the tests, the water was affected by drilling mud as indicated by the high pH (8.9) and high bicarbonate concentration (359 mg/L) that both resulted from the impact of sodium carbonate (Na₂CO₃) additives. The high amount of dissolved organic matter (>?58 mg/L) and its molecular-weight distribution pattern indicated that residues of cellulose, an ingredient of the drilling mud, were still present at the end of the tests. Clear evidence of this contamination gave the measured uranine that was added as a tracer into the drilling mud. During fluid production, the microbial community structure and abundance changed and correlated with the content of drilling mud. Eight taxa of sulfate-reducing bacteria, key organisms in processes like bio-corrosion and bio-clogging, were identified. It can be assumed that their activity will be affected during usage of the reservoir.  相似文献   

The chronostratigraphy of a quaternary sequence at the distal part of the nile littoral cell,Haifa Bay,Israel     

Simona Avnaim‐Katav  Ahuva Almogi‐Labin  Amir Sandler  Dorit Sivan  Naomi Porat  Ari Matmon 《第四纪科学杂志》2012,27(7):675-686

The current detailed chronostratigraphic framework of the last 1 Ma of an eastern Mediterranean sequence (Haifa Bay, Israel) aims to examine the relative roles of sea‐level changes, climate and tectonics. Seven continuous marine cores, up to ～120 m long, were recovered from shallow water depths. The cores were dated by optically stimulated luminescence, ¹⁴C, magnetostratigraphy, ²³⁰Th/²³⁴U, ²⁶Al/¹⁰Be, occurrence of index fossils and correlated to the global sea‐level curve and Marine Isotope Stages (MIS). The sedimentary sequence accumulated during the last ca. 1.0 Ma consists of 21 transgression–regression units with hiatuses between them. Five marine/terrestrial cycles, which occur in the lower part of the sequence, are attributed to the Jaramillo subchron and the Brunhes–Matuyama boundary, and correspond to MIS 29–21. The top ～50 m includes three sedimentary cycles deposited in the last ca. 400 ka. The regressive phases during this interval correspond to Glacial MIS 8, 6 and 2, while the transgressions correspond to Interglacial MIS 11, 7, 5 and 1. Thus, for the first time, this study documents the longest Quaternary succession dated so far in a key area of the Levant, sensitive to global history of sea‐level changes and glacial/interglacial fluctuations. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   

Qualitative and quantitative aspects of the evolution of the planktonic populations in Lake Lugano   总被引：2，自引：0，他引：2  

Bruno Polli  Marco Simona 《Aquatic Sciences - Research Across Boundaries》1992,54(3-4):303-320

This study is a summary of all available information on the long-term and on the more recent development of the planktonic populations in Lago di Lugano with regard to its trophic evolution. The first effects of the lake eutrophication became manifest beginning in the 1950's and brought to important changes in the planktonic community as the appearance and the rapid increase of the filamentous algaeOscillatoria rubescens andStephanodiscus hantzschii, and the disappearance of the zooplanktonic Diaptomidae. Since 1980 the phosphorus concentration began to decrease in lake's epilimnion and the planktonic community showed a new composition, evidencing the presence of others predominant species:Oscillatoria redekei, Lyngbya limnetica, Stephanodiscus sp. (little form). Since 1989 algal standing crop reduced its value below 2 g m^–2 (dry weight); furthermore a strong decrease of the Cyanophceae and the appearance of new predominant species (Tabellaria fenestrata, div. Ulotrichales) occurred. At the same time an increase of the herbivorous zooplankton (Daphnia hyalina) and the come-back of the Diaptomidae was observed. Only in the northern basin a decrease tendency of the primary production was observed, changing from 480 to about 300 g C m^–2 yr^–1 during the last 10 years.  相似文献   

The Calabrian Stage redefined     

Maria Bianca Cita Luca Capraro Neri Ciaranfi Enrico Di Stefano Fabrizio Lirer Patrizia Maiorano Maria Marino Isabella Raffi Domenico Rio Rodolfo Sprovieri Simona Stefanelli Gian Battista Vai 《《幕》》2008,31(4):408-419

The name Calabrian was introduced in the geological literature by the French stratigrapher Maurice Gignoux in 1910, and later described in his important monograph （633 pages） ＂Les formations marines pliocknes et quaternaires de l＇ltalie du sud et de la Sicile ＂published in 1913. Detailed data were provided on several sections （Santa Maria di Catanzaro, Caraffa, Monasterace, Palermo） and on their fossil content. The Calabrian Stage has commonly been used for over fifty years as the oldest subdivision of the Qua- ternary, notably in the time scales of Berggren ＆ van Cou- vering （1974） and Haq ＆ Eysinga （1987）. However, after the GSSP for the Pliocene/Pleistocene boundary （P/P） was approved by INQUA in 1982 and ratified by lUGS in 1984 at the Vrica section of Calabria, there was a decline in the usage of the stage name, and an increasing tendency by many Quaternary workers to question the boundary stratotype. This was because there was increasing evidence that it did not correspond to the beginning of the ＂ice age＂. In doing so, they were not complying with the recommendations presented at the 18th International Geological Congress （IGC） in London, 1948 （Oakley, 1950）.  相似文献   

Speciation of naturally-accumulated uranium in an organic-rich soil of an alpine region (Switzerland)     

Simona Regenspurg  Camille Margot-Roquier  Pascal Froidevaux  Pilar Junier 《Geochimica et cosmochimica acta》2010,74(7):2082-2098

Very high concentrations of uranium (up to 4000 ppm) were found in a natural soil in the Dischma valley, an alpine region in the Grisons canton in Switzerland. The goal of this study was to examine the redox state and the nature of uranium binding in the soil matrix in order to understand the accumulation mechanism. Pore water profiles collected from Dischma soil revealed the establishment of anoxic conditions with increasing soil depth. A combination of chemical extraction methods and spectroscopy was applied to characterize the redox state and binding environment of uranium in the soil. Bicarbonate extraction under anoxic conditions released most of the uranium indicating that uranium occurs predominantly in the hexavalent form. Surprisingly, the uranium redox state did not vary greatly as a function of depth. X-ray absorption near edge spectroscopy (XANES), confirmed that uranium was present as a mixture of U(VI) and U(IV) with U(VI) dominating. Sequential extractions of soil samples showed that the dissolution of solid organic matter resulted in the simultaneous release of the majority of the soil uranium content (>95%). Extended X-ray absorption fine structure (EXAFS) spectroscopy also revealed that soil-associated uranium in the soil matrix was mainly octahedrally coordinated, with an average of 1.7 axial (at about 1.76 Å) and 4.6 to 5.3 equatorial oxygen atoms (at about 2.36 Å) indicating the dominance of a uranyl-like (UO₂²⁺) structure presumably mixed with some U(IV). An additional EXAFS signal (at about 3.2 Å) identified in some spectra suggested that uranium was also bound (via an oxygen atom) to a light element such as carbon, phosphorus or silicon. Gamma spectrometric measurements of soil profiles failed to identify uranium long-life daughter products in the soil which is an indication that uranium originates elsewhere and was transported to its current location by water. Finally, it was found that the release of uranium from the soil was significantly promoted at very low pH values (pH 2) and increased with increasing pH values (between pH 5 and 9).  相似文献