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A new C15 acetogenin, cyclic enyne (12E)‐cis‐maneonene‐E ( 1 ) and (12Z)‐trans‐maneonene‐B ( 2 ), together with different secondary metabolites including sesquiterpenoid 2,10‐dibromo‐3‐chloro‐α‐chamigrene ( 3 ) and fatty acid aldehydes ( 4–8 ) were isolated from the petroleum ether extract of a red alga, identified as Laurencia papillosa, collected from the Saudi Red Sea coast. The L. papillosa crude extract and its active ingredient acetogenin ( 1 ) have been evaluated for the ability to control two main economically important pests, the confused flour beetle larvae Tribolium confusum and Culex pipiens mosquito larvae. Compound 1 showed high potential as a natural insecticide against these two pests. In the case of T. confusum larvae, the LD50 values were 0.21 and 0.16% after 6 and 12 days of treatment with crude extract and acetogenin, respectively. On the other hand, the resulting LC50 values of mosquito larvae bioassay were 30.7, 36.9, and 41.8 ppm after 24 h treatment against 2nd, 3rd, and 4th larval instars, respectively. Also, an increase of the insecticidal effects against mosquito larvae was recorded after extending treatment time to 48 h. 相似文献
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The muscle myogen proteins of 10 species of the family Galaxiidae exhibited a high degree of species specificity when separated by starch gel electrophoresis. Non‐diadromous, non‐lacustrine species had some variation in the proteins present, while diadromous and lacustrine species, except for Galaxias maculatus, had remarkably uniform muscle myogen electrophero‐grams. The variation can be explained by polymorphism and mosaic evolution of isolated freshwater populations. It was found that previously erected specific and generic categories, except for G. vulgaris, are clearly defined by differences in muscle protein. The standardised electropherogram types obtained were subjected to cluster analysis using both squared Euclidean distance and the complement of Jacard's coefficient with average sorting strategy. A principal component analysis was also carried out. The first two components of the principal component analysis widely spaced the three genera included in this study (Salmo, Galaxias, and Neochanna), with N. burrowsius occupying an intermediate position between Galaxias and Neochanna. Previous workers have considered this species to be a connecting link between the two genera. The dendrogram produced by using squared Euclidean distance (which included all available information) first clustered the types into Salmoniidae and Galaxiidae. The latter were split into the genus Neochanna and the genus Galaxias which was then subdivided into stout‐bodied species (G. argenleus and G. jasciatus) and slender‐bodied species. G. brevipinnis and G. vulgaris types were further separated from the three alpine Galaxias species and G. maculatus. The substantial agreement of these results with previous taxonomic efforts indicates that electrophoretic data can be a valid taxonomic tool. 相似文献
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The dytiscid beetle Rhantus sikkimensis, Regimbart, 1899 (Coleoptera: Dytiscidae), a member of the freshwater insect communities of the Darjeeling Himalayas, were noted to predate on the coexisting larvae of Chironomus sp. Meigen. Evaluation of predation by R. sikkimensis on Chironomus sp. larvae, in the laboratory, revealed that a single adult morph of R. sikkimensis could kill and consume on an average 10–90 and 10–78 numbers of small and large Chironomus sp. larvae, respectively, per day, depending on the prey density. The attack rate ranged between 520 and 537, and the handling time ranged between 4.3 and 8.6 depending on the size of the preys. The predation varied with respect to predator density also, with a maximum of 151 larvae killed by three predators per day. Two indices of predation, ingestion rate (IR) ranging between 13.33 and 74.15 larvae/day/predator and clearance rate (CR) ranging between 19.67 and 39.99 L prey/day/predator, varied with the prey size and predator density, significantly, when the predation was observed for 9 consecutive days, at two predator densities. It was also noted that R. sikkimensis predated on an average 9.8 larvae of Chironomus sp. and 1 larva of Culex sp., when the larvae of both the species are present together as preys, showing a preference for the Chironomus sp. larvae. 相似文献
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