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ZHAN Jiajia XU Huiwen CAI Hongliang .College of Construction Engineering Jilin University Changchun China .China Water Northeastern Investigation Design Research Co.Ltd Changchun China 《东北亚地学研究》2009,12(1):32-35
Drilling fluid additives has a major impact on rheology behavior and other performances of the drilling fluid. The key to low temperature drilling fluids is choosing antifreeze and polymer agent which have a strong ability in resisting low temperature. On the basis of determining glycol as antifreeze agent, the low-temperatureperformance and capacities of anti-collapse of regular validity mud agents have been studied, the mechanism of anti-caving of the partially hydrolyzed polyacrylamide （PHPA）, polypropylene potassium （KPA） and PAC-141 polymer additives have been also analyzed. A pilot study of compatibility between ethylene glycol and the polymers has educed that the polymers, ethylene glycol and inorganic salt are compatible, and they can significantly reduce the freezing point of drilling fluid. Anti-collapse ability of the drilling mud is the results of synergistic action among the agents. 相似文献
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本文研究了六种抗冻保护剂GLY(甘油)、DMSO(二甲基亚砜)、PG(丙二醇)、EG(乙二醇)、DMA(二甲基乙酰胺)和Me OH(甲醇)在三种不同浓度下(5%、10%、15%,v/v)对太平洋牡蛎(Crassostrea gigas)担轮幼虫的毒性作用及冷冻保存的影响。太平洋牡蛎担轮幼虫毒性实验证明:抗冻保护剂的种类和浓度对太平洋牡蛎担轮幼虫产生的毒性强弱不同,在抗冻保护剂浓度为5%时,GLY、DMSO、PG、EG对太平洋牡蛎的毒性作用显著低于其他抗冻保护剂(P0.05);随着抗冻保护剂浓度增加到10%,GLY、DMSO、EG、Me OH对太平洋牡蛎担轮幼虫的毒性作用显著低于其他抗冻保护剂(P0.05);而抗冻保护剂浓度为15%时,GLY、DMSO对太平洋牡蛎担轮幼虫的毒性作用显著低于其他抗冻保护剂(P0.05),且随着抗冻保护剂浓度的升高,其对太平洋牡蛎担轮幼虫的毒性作用也增大。太平洋牡蛎担轮幼虫的超低温冷冻保存实验证明:以10%DMSO作为抗冻保护剂,采用分布降温法冷冻保存太平洋牡蛎担轮幼虫,28°C水浴解冻后担轮幼虫运动率达到(73.00±2.00)%,显著高于其他实验组(P0.05),其次为GLY组,且各浓度间差异不显著(P0.05)。该研究为太平洋牡蛎及其他贝类胚胎保存提供了科学依据,在贝类遗传育种和生物技术等方面具有一定的应用前景,具有重要的生产实践价值。 相似文献
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结合高原冻土层条件,研究钻井液的耐低温特性,进行抗低温研究,初步确定了耐低温钻井体系,可供高原冻土层钻探施工借鉴使用。 相似文献
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天山寒区冰缘植物珠芽蓼叶片抗冻蛋白的发现 总被引：18，自引：5，他引：13
以生长在天山海拔3450m处的珠芽蓼（Polygonum viviparum)为实验材料,从叶片中提取质外体蛋白,经SDS-PAGE分析,发现在分子量为15.2～72.3kD范围内出现七条多肽,PAS染色显示这些多肽中均含有糖基,通过光镜组织切片显示,在珠芽蓼叶片细胞的质外体中存在丰富的蛋白质,PAS染色确定其含有糖蛋白,通过测定其热滞值,进一步确定质外体蛋白是具有抗冻活性的糖蛋白,说明抗冻蛋白是维持珠芽蓼抗冻性的重要物质基础。 相似文献
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Drilling fluid additives has a major impact on rheology behavior and other performances of the drilling fluid. The key to low temperature drilling fluids is choosing antifreeze and polymer agent which have a strong ability in resisting low temperature. On the basis of determining glycol as antifreeze agent, the low-temperature-performance and capacities of anti-collapse of regular validity mud agents have been studied, the mechanism of anti-caving of the partially hydrolyzed polyacrylamide (PHPA), polypropylene potassium (KPA) and PAC-141 polymer additives have been also analyzed. A pilot study of compatibility between ethylene glycol and the polymers has educed that the polymers, ethylene glycol and inorganic salt are compatible, and they can significantly reduce the freezing point of drilling fluid. Anti-collapse ability of the drilling mud is the results of synergistic action among the agents. 相似文献
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