Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri     

YU Ziniu  WEI Xiaohu  KONG Xiaoyu  YU Shanshan 《海洋学报(英文版)》2007,26(1):93-100

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.  相似文献   

基于MAPGIS的军事标图系统的设计   总被引：2，自引：0，他引：2  

张剑波  陈宇等 《地球科学》2002,27(3):301-305

军事领域是一个非常特殊的行业，信息的安全性和空间数据的多样性是应用开发的重要问题。在全球信息化日趋高速发展和逐步成熟的今天，军队信息化建设也必须与GIS技术紧密结合，跟上时代前进的步伐。较系统地研究了GIS技术在军事标图绘制中的应用和集成问题，并提供了相应的理论方法和实现系统。在此基础上介绍了以MAPGIS为开发平台的军事标绘系统的实用功能和特点。首先分析了军事信息系统对地理信息数据的要求，并且评述了相关领域的研究现状。然后较为系统地介绍了军事标图系统的总体设计，根据军事标绘对象的数据特征，设计符合要求的应用系统提出了面向对象和无缝交互工具等的设计思想。对军标对象数据模型和操作模型进行了详细地阐述和说明，并将这些模型在系统中进行了实现。最后介绍了军事标图系统的主要模块和功能，探讨了今后GIS研究和发展的方向。  相似文献   

Deformation patterns of upper Quaternary strata and their relation to active tectonics,Po Basin,Italy     

Alessandro Amorosi  Luigi Bruno  Bruno Campo  Bianca Costagli  Wan Hong  Vincenzo Picotti  Stefano Claudio Vaiani 《Sedimentology》2021,68(1):402-424

Despite increased application of subsurface datasets below the limits of seismic resolution, reconstructing near‐surface deformation of shallow key stratigraphic markers beneath modern alluvial and coastal plains through sediment core analysis has received little attention. Highly resolved stratigraphy of Upper Pleistocene to Holocene (Marine Isotope Stage 5e to Marine Isotope Stage 1) alluvial, deltaic and coastal depositional systems across the southern Po Plain, down to 150 m depth, provides an unambiguous documentation on the deformation of previously flat‐lying strata that goes back in time beyond the limits of morphological, historical and palaeoseismic records. Five prominent key horizons, accurately selected on the basis of their sedimentological characteristics and typified for their fossil content, were used as highly effective stratigraphic markers (M1 to M5) that can be tracked for tens of kilometres across the basin. A facies‐controlled approach tied to a robust chronology (102 radiocarbon dates) reveals considerable deformation of laterally extensive nearshore (M1), continental (M2 and M3) and lagoon (M4 and M5) marker beds originally deposited in a horizontal position (M1, M4 and M5). The areas where antiformal geometries are best observed are remarkably coincident with the axes of buried ramp anticlines, across which new seismic images reveal substantially warped stratal geometries of Lower Pleistocene strata. The striking spatial coincidence of fold crests with the epicentres of historic and instrumental seismicity suggests that deformation of marker beds M1 to M5 might reflect, in part at least, syntectonically generated relief and, thus, active tectonism. Precise identification and lateral tracing of chronologically constrained stratigraphic markers in the ¹⁴C time window through combined sedimentological and palaeoecological data may delineate late Quaternary subsurface stratigraphic architecture at an unprecedented level of detail, outlining cryptic stratal geometries at the sub‐seismic scale. This approach is highly reproducible in tectonically active Quaternary depositional systems and can help to assess patterns of active deformation in the subsurface of modern alluvial and coastal plains worldwide.  相似文献   

鄂尔多斯盆地前寒武纪末期古地貌恢复及其对烃源岩的控制作用   总被引：1，自引：0，他引：1  

侯林君  陈洪德  罗林军  张成弓  赵俊兴  苏中堂 《地球科学与环境学报》2019,(4):475-490

鄂尔多斯盆地前寒武纪末期古地貌控制着后期沉积体系的空间配置与古地理展布。通过野外剖面、岩芯分析、测井数据等建立鄂尔多斯盆地寒武系层序地层格架;选取寒武纪SS1最大海泛面(徐庄组沉积晚期地层)为标志层,通过残余厚度印模法,并结合古地质图分析,对研究区前寒武纪进行古地貌恢复,明确了前寒武纪沉积背景、构造格局及物源方向。总体上,前寒武纪鄂尔多斯盆地南、北濒临秦祁海和兴蒙海,东、西被贺兰和晋豫陕坳拉槽所夹持,古地貌东北高、西南低,受秦祁海影响最大,可将其划分为3个古构造地貌单元:高地剥蚀区、受古构造控制形成的坳陷区以及处于剥蚀与沉积过渡地带的斜坡阶地区。通过研究前寒武纪末期古地貌与寒武纪早期烃源岩分布认为,拉张活动背景下的坳陷带以及斜坡带是鄂尔多斯盆地寒武纪早期烃源岩发育的有利区块,前寒武纪古地貌格局影响了寒武纪早期烃源岩发育与分布。恢复古地貌形态对于揭示物源供给,研究沉积物搬运、平面和纵向展布特征,探究沉积、沉降中心的迁移规律,以及进一步揭示生储盖组合和分布具有重大意义。  相似文献   

裙带菜配子体基因工程选择标记的研究   总被引：1，自引：1，他引：0  

张亦陈  高江涛  张喆  姜鹏  秦松 《海洋科学》2007,31(12):64-68

研究了裙带菜(Undaria pinnatifida)配子体对除草剂草丁膦的敏感性以及温度对草丁膦处理效果的影响。结果表明,裙带菜配子体对草丁膦敏感;统计学分析得出了草丁膦在不同温度条件下处理裙带菜配子体的半致死浓度(LD50)及95%可信限;温差达到10℃时,相同处理时间各组内不同处理温度样品的LD50之间呈现显著差异,第6天的LD50由10℃组的15.78 mg/L降为20℃组的11.56 mg/L和25℃组的10.33 mg/L。研究发现可以尝试采用草丁膦作为裙带菜配子体遗传转化的选择试剂。  相似文献   

Methods Comparison for Microsatellite Marker Development： Different Isolation Methods, Different Yield Efficiency     

Aibin Zhan  Zhenmin Bao  Xiaoli Hu  Wei Lu  Jingjie Hu 《中国海洋大学学报(英文版)》2009,8(2):161-165

Microsatellite markers have become one kind of the most important molecular tools used in various researches. A large number of microsatellite markers are required for the whole genome survey in the fields of molecular ecology, quantitative genetics and genomics. Therefore, it is extremely necessary to select several versatile, low-cost, efficient and time- and labor-saving methods to develop a large panel of microsatellite markers. In this study, we used Zhikong scallop (Chlamys farreri) as the target species to compare the efficiency of the five methods derived from three strategies for microsatellite marker development. The results showed that the strategy of constructing small insert genomic DNA library resulted in poor efficiency, while the microsatellite-enriched strategy highly improved the isolation efficiency. Although the mining public database strategy is time- and cost-saving, it is difficult to obtain a large number of microsatellite markers, mainly due to the limited sequence data of non-model species deposited in public databases. Based on the results in this study, we recommend two methods, microsatellite-enriched library construction method and FIASCO-colony hybridization method, for large-scale microsatellite marker development. Both methods were derived from the mi-crosatellite-enriched strategy. The experimental results obtained from Zhikong scallop also provide the reference for microsatellite marker development in other species with large genomes.  相似文献   

黄颡鱼(Pelteobagrus eupogon)不同生态地理分布群体遗传多样性的微卫星分析   总被引：1，自引：0，他引：1  

李大宇  殷倩茜  侯宁  孙效文  梁利群 《海洋与湖沼》2009,40(4):460-469

利用40个黄颡鱼微卫星分子标记对吉林省月亮湖野生黄颡鱼(YL,30尾)、四川野生黄颡鱼(SC,30尾)、黑龙江省哈尔滨市松花江段黄颡鱼(SH,18尾)、湖北省荆州市长湖野生黄颡鱼子代(YY,30尾)及其亲本(QQ,30尾)、天津市换新国家级原良种场黄颡鱼1号(TJ,30尾)6个黄颡鱼群体的观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)和有效等位基因数(Ae)等进行了遗传检测,根据基因频率计算遗传相似系数和Nei氏标准遗传距离,x2检验估计Hardy-weinberg平衡,采用近交系数(FST)和基因流(Nm)分析群体的遗传分化及其来源.同时,使用PHYLIP3.63软件绘制基于Nei氏标准遗传距离的UPGMA进化树.6个群体共检测到5042个扩增片段,长度在150-650bp,40个基因座扩增出等位基因数从1-8个不等,共计143个等位基因,平均每个基因座扩增得到3.575个等位基因.结果显示:(1)6个黄颡鱼群体的多态性指标均适中,PIC在0.00-0.79之间,平均值为0.40、0.37、0.35、0.39、0.35和0.19,有效等位基因数(Ae)1.00-5.44个不等,平均有效等位基因数依次为2.05、2.30、1.93、2.07、1.93和1.47,无偏期望杂合度(He)在0.00-0.83之间,平均值为0.45、0.46、0.36、0.46、0.41和0.23;(2)遗传相似系数YY与QQ遗传相似度最高(0.9947),TJ与SC遗传相似系数最低(0.4846),聚类结果与地理分布呈一定相关性.  相似文献   

皱纹盘鲍中国群体和日本群体的自交与杂交F₁ 的RAPD 标记   总被引：47，自引：8，他引：47  

张国范  王继红  赵洪恩  阙华勇  刘晓 《海洋与湖沼》2002,33(5):484-491

利用皱纹盘鲍中国群体和日本群体野生个体的单自交和单正反杂交获得了 4个F1家系 ,其中家系AJC、BCC、CCJ、DJJ分别为日本♀×中国♂、中国♀×中国♂、中国♀×日本♂和日本♀×日本♂组合 ,采用 2 2个引物对上述四个家系及其各自亲本个体的遗传结构进行了RAPD分析。结果表明 ,四个家系的平均杂合度 :AJC为 0 .2 32 9,BCC为 0 .1 667,CCJ为 0 .1 773,DJJ为 0 .1 649;各家系两亲本间遗传距离 :AJC为 0 .2 4 62 ,BCC为 0 .1 70 1 ,CCJ为 0 .2 1 0 9,DJJ为 0 .1 688。各家系子代群体与父母本的遗传距离 :AJC为 0 .1 772和 0 .2 2 2 4 ,BCC为 0 .1 1 81和 0 .1 92 0 ,CCJ为0 .1 1 2 3和 0 .1 691 ,DJJ为 0 .0 694和 0 .1 947。各家系子代个体间的遗传距离AJC为 0 .0 976,BCC为0 .0 95 1 ,CCJ为 0 .0 699,DJJ为 0 .0 682 ,而子代各群体间遗传距离均小于 0 .0 2 2 0。  相似文献   

花岗岩构造研究及花岗岩构造动力学刍议   总被引：15，自引：1，他引：14       下载免费PDF全文

王涛  王晓霞  郑亚东  洪大卫  王新社 《地质科学》2007,42(1):91-113

花岗岩可以视为一种很好的构造标志体,犹如褶皱、断裂一样。从花岗岩浆的形成、融体分离、岩浆上升到岩体定位以及变形改造的全过程都蕴含着丰富的构造动力学信息。研究花岗岩浆上升、迁移和定位可以探讨构造块体抬升及区域构造动力学。岩体生长方式与构造块体的运动学、动力学有密切的关系,极性生长揭示了上、下构造块体或岩石圈之间的相对的近水平方向剪切运移。变形花岗岩体是一种区域尺度的应变标志体,可以进行岩石有限应变测量和流变学参数估算,为分析区域构造变形特征提供应变参数。以对不同期次、不同变形程度花岗岩体为间接标志体,通过锆石定年可以限定变形的时间,特别是有可能确定早期变形的时间。岩体定位深度的系统研究有利于了解构造块体的抬升和深部构造作用。花岗岩构造与花岗岩成因类型特别是其演变研究的结合是判别构造块体动力学背景以及其转换的有效途径。通过这几方面的系统研究和有机结合,可以提供丰富的构造动力学信息,是否可能发展成较系统的花岗岩构造动力学值得探讨。  相似文献   

被动椭圆形标志物应变分析的计算机模拟   总被引：1，自引：0，他引：1  

何绍勋  王焕金 《大地构造与成矿学》1991,15(3):233-242

本文采用计算机数学模拟的方法更深入地探讨了椭圆形标志物应变分析技术(R_f/Φ去应变法、Shimamoto等提出的代数法、调和平均值法和算术平均值法)的适用条件。一共形成了十一组有代表性的应变前数组,四种变形路径:纯剪、单剪、纯剪加单剪和体变。当应变前数组在(-90°,90°)内均匀分布或随机分布时,无论变形路径是哪一种(共轴的还是非共轴的),上述的所有方法都是适用有,且以代数法最佳。当应变前数组具有优选方位(单峰分布)且变形路径为共轴递进变形时,在一定条件下,R_f/Φ法能用来估计应变。经分析发现当应变足够大时,调和平均值法是一种简单快速的应变计算法。  相似文献