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Isolation, cloning and sequencing of AFLP markers related to disease-resistance traits in Fenneropenaeus chinensis 总被引：1，自引：0，他引：1
Amplified fragment length polymorphisms （AFLP） technique was used to analyze the fingerprinting of four successive generations of Fenneropenaeus chinensis to reveal their disease-resistance traits. Some loci showed quite different genetic frequencies due to artificial selection, which implied that these fragments were putative markers related to the disease-resistance trait. We developed a simple and effective method to further characterize these AFLP fragments. Specific AFLP bands were cut directly from polyacrylamide gels, re-amplified, cloned and sequenced. Eight putative genetic markers were sequenced and their sizes ranged from 63 to 209 bp. The sequences were submitted to dbGSS （database of Genome Sequence Survey）; and the BLAST analysis showed low similarity to the function genes, indicating these markers were tightly linked to a disease-resistance trait but were not functional genes. 相似文献
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1 INTRODUCTION Silver carp (Hypophthalmichthys molitrix) is an important species in freshwater fisheries and ranks the first in world fish production (Li, 1993). China has a long history of aquaculture of silver carp that is very difficult for selective breeding because it has a long life cycle. However, development of cytoge-netics and modern molecular genetics built up a strong impact to the research on selective breeding. A rapid, efficient approach for establishment of a pure line of… 相似文献
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本文介绍了成都市地震监测台网中心系统构成,为节省微机安装时间和方便工作人员操作,重点介绍了计算机克隆技术在地震监测中心台网系统维护中的应用。 相似文献