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采用Wilbert蛋白银法及Chatton—Lwoff银浸法对一种附生于栉孔扇贝鳃丝上的寄生车轮虫进行了光镜水平下的研究。依据其形态学和纤毛图式特征,该种被鉴定为TrichodinajadranicaRaabe,1958.此为我国新记录,同时也是第一例有关外寄生于扇贝鳃表纤毛虫原生动物的报道。 相似文献
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通过多种化学分析方法 ,初步探讨了扇贝肝胰腺组织的化学成分。结果表明 :该组织中含有较丰富的营养物质 ,并含有重要生理活性的 EPA和 DHA,微量元素含量也很丰富。这些结果为扇贝加工后下脚料的利用 ,提供了基础资料 相似文献
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长岛养殖扇贝丰,欠原因的初步探讨 总被引:1,自引:0,他引:1
通过对长岛扇贝养殖环境两年(1989、1990)监测及历史资料的初步分析研究,得出了影响长岛养殖扇贝丰欠的主要因子有:1)长岛地区的降水量;2)海水中浮游植物含量;3)海水盐度。建立了适合长岛水域的模糊综合评判关系式用以综合评判长岛的扇贝养殖环境及其与养殖扇贝丰、欠之间的关系。 相似文献
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Sequence variation of the first internal transcribed spacer of ribosomal DNA ( ITS - 1 ) was examined and its application to the study of genetic variation was explored in four populations of farter' s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA (analysis of molecular variance) showed that the majority (96.26%) of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 ( = 0.042), indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly. 相似文献
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海洋贝类利用模式生命周期评价方法研究 总被引:2,自引:0,他引:2
首次将产品生命周期评价方法(LCA)应用于海洋贝类利用模式上。根据海洋贝类利用技术产业发展的特性和趋势,设计构建了由确定目标和范围、清单分析、影响评价3个步骤组成的评价体系,挑选了具有典型代表的2种扇贝利用模式进行评价,对其生产过程中的资源消耗、固体废弃物、富营养化、温室效应、酸化影响和潜在影响进行对比评价。评价结果显示其中资源消耗、温室效应、酸化影响、潜在健康影响的影响潜值模式2(产品模式为扇贝柱、复合氨基酸、鱼虾饵料和贝壳工艺品)比模式1(产品模式为扇贝柱、食用贝边、鱼虾鲜饵料和饲料添加剂)低;而固体废弃物、富营养化的影响潜值,模式2比模式1高。本评价方法可用于选择和优化海洋贝类的绿色化高值利用模式。研究表明,利用文章提出的海洋贝类利用评价方法可以有效掌握贝类利用的整个过程的环境行为,确定其中优化资源、节省能源和减少污染的关键步骤,为优化利用模式提供基础数据支持。 相似文献
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高温刺激导致虾夷扇贝死亡因素的探究 总被引:2,自引:0,他引:2
本文探究了高温刺激导致一龄和二龄虾夷扇贝死亡的原因。在高温刺激以及恢复过程中,两种贝龄的扇贝存活率存在显著差异(P0.05),同时两者的谷丙转氨酶(glutamic-pyruvic transaminase,GPT)活性、p53蛋白含量、总抗氧化能力(total antioxidant capacity,TAOC)以及HSP70(heat shock protein 70)含量在高温刺激以及恢复过程中的变化均存在显著差异。而且这些酶的活性(TAOC活性除外)以及蛋白质含量(p53以及HSP70)均受到贝龄、处理时间以及两者综合作用的显著影响。经过Cox模型分析可知,虾夷扇贝受到高温刺激后的存活受到贝龄、GPT活性、TAOC活性、p53含量以及HSP70含量的显著影响。此外,高温刺激会影响扇贝的心跳频率以及心输出量,从而影响心脏对机体的供氧供血能力,影响扇贝对高温的适应能力。 相似文献
9.
Molecular identification of scallop planktonic larvae using species-specific microsatellites 总被引:3,自引:0,他引:3
ZHAN Aibin HU Xiaoli BAO Lisui LU Wei PENG Wei WANG Mingling HU Jingjie 《海洋学报(英文版)》2008,27(5):134-146
The identification of scallop larvae is essential to understand the population structure and community dynamics and to assess the potential environmental impacts caused by scallop larvae released or escaped. However, the larvae identification by morphological characteristics is notoriously difficult, mainly due to the small size (usually being less than 150 μm) and vague morphological characteristics among different scallop species. A simple and accurate molecular method was developed to identify four economically farmed scallop species, the Zhikong scallop Chlamys farreri, the noble scallop C. nobilis, the bay scallop Argopecten irradians and the Yesso scallop Mizuhopecten yessoensis. The tests used the high degree of species-specific microsatellite markers, which was specified by transferability analyses, assessed by reference individuals and evaluated by BLAST searches. The sensitivity test indicated that the species-specific microsatellites were sensitive enough for the detection of 1% -2% larvae in mixed plankton samples. Larvae collected from scallop hatcheries and their effluents and from the artificially controlled crosses were well identified to the species/hybrid level. The results demonstrated that the one-step PCR-based assay was technically simple, inexpensive and robust in identification analyses, and also less sensitive to initial quality of template DNA extracted from the ethanol-preserved samples for several years. 相似文献
10.
Two Large-insert genomic bacterial artificial chromosome (BAC) libraries of Zhikong scallop Chlamys farreri were constructed to promote our genetic and genomic research. High-quality megabase-sized DNA was isolated from the adductor muscle of the scallop and partially digested by BamH I and Mbo I, respectively. The BamH I library consisted of 53 760 clones while the Mbo I library consisted of 7 680clones. Approximately 96 % of the clones in BamH I library contained nuclear DNA inserts in average size of 100 kb, providing a coverage of 5.3 haploid genome equivalents. Similarly, the Mbo I library with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1 haploid genome equivalents. 相似文献