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This study is part of a project aiming to validate the use of the intertidal shanny Lipophorys pholis as a sentinel species in pollution monitoring in NW European marine ecosystems. To this end, a characterisation of acethylcholin (AChE), butyrylcholin (BChE) and propionylcholin (PrChE) esterases in L. pholis muscle was performed and the results indicated that AChE was predominant. Furthermore, the use of eserine sulphate and BW284c51 (0.64–800 μM), and iso-OMPA (0.08–16 mM), confirmed the measurement of true cholinesterases (ChEs) as well as the presence of pseudocholinesterases. The field application of these markers to L. pholis, sampled in seven locations along the Portuguese coast, revealed that fish were likely to be affected by neurotoxic compounds. This was indicated by the significant depletion of AChE (p < 0.05) in animals collected at urban and industrialised sites, compared with those from reference locations. The inclusion of a marker of effect, measured as lipid peroxidation levels in muscle tissue, also revealed the existence of site differences. Overall, the study further validates the utility of L. pholis in pollution monitoring studies. 相似文献
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The first use of Fulton's K for assessing and comparing the conditions of inter‐tidal fish populations 下载免费PDF全文
Fulton's K condition factor was applied, for the first time, to inter‐tidal specimens of the shanny (Lipophrys pholis) and long‐spined scorpion fish (Taurulus bubalis) from two English rocky shore and two Welsh rocky shore sites during summer 2010 and winter 2011. As both species contribute to the diet of commercial species such as cod (Gadus morhua) and near‐threatened species such as the European otter (Lutra lutra), their condition may affect that of these predators. Fulton's K found that inter‐tidal Welsh fish maintained a ‘good’ condition between seasons, whereas the inter‐tidal English fish were in a poorer condition during winter. Although condition also changed amongst the sites on each coast, further studies are needed into fish morphologies, environmental parameters, prey availabilities and abundances, and fish specimen sex and maturities. 相似文献
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The temporal patterns of feeding activity of Lipophrys pholis and Coryphoblennius galerita were studied by snorkelling dives during high-waters. The feeding activity was especially intense during the rising-tide when compared to the ebbing-tide. The relative abundances of C. galerita and small L. pholis observed on rock-walls were higher during the rising- and high-tide. However, L. pholis larger than 7–8 cm showed higher relative abundance on rock-walls during the ebbing phase, apparently because they move up and down with the tide, and many individuals were in their downward movements as the tide ebbs. It is hypothesized that the asymmetry in feeding pattern observed in both species reflects a trade off between two conflicting needs, maximizing feeding time and reaching their low-tide shelters in time, avoiding the risk of getting stranded in unsuitable locations. 相似文献
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