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1.
本文分别对雌雄白缘(鱼央)的5S rDNA进行了PCR扩增和序列分析,并采用双色荧光原位杂交(FISH)技术,以白缘(鱼央)的5S rDNA序列和小麦的45S rDNA为探针,对其在白缘(鱼央)雌雄中期染色体上进行了FISH定位研究。结果表明,白缘(鱼央)5S rDNA序列无雌雄差异;5S rDNA的保守区序列为117 bp;5S rDNA和45S rDNA分别被定位于白缘(鱼央)的性染色体和第5号染色体上。同时从GenBank中获得了22种鱼的5S rDNA,运用DNAman软件构建了23种鱼的系统发育树,对白缘(鱼央)的进化地位进行了初步研究。本研究为阐明白缘(鱼央)在鱼类系统进化中的位置、重复序列在脊椎动物性染色体上的分布状况以及与性别决定与分化的关系,提供了资料积累和分析依据。 相似文献
2.
《African Journal of Marine Science》2013,35(1):5-35
This paper gives an overview of the main living marine resources of Namibia. It focuses on the scientific research conducted during the past decade as input to the management of these resources. The distribution and habitats of the most important harvested species and the main seabird populations are briefly described and discussed. The life histories of the major exploited species are summarized, with emphasis on spatial and temporal spawning patterns, dispersal of early life stages, migration patterns of recruits and adults, and diet, the latter particularly as it relates to potential competition between species. A number of commercially important species, such as the hake Merluccius capensis and M. paradoxus, deep-sea red crab Chaceon maritae, West Coast rock lobster Jasus lalandii, skipjack tuna Katsuwonus pelamis, southern albacore Thunnus alalunga and to a lesser extent Cape horse mackerel Trachurus capensis, southern African sardine Sardinops sagax and Cape anchovy Engraulis capensis, are distributed across national boundaries, requiring regional cooperation in research and management. The history and current status of the major fisheries is discussed. Over the past 30–40 years total annual catches have declined from a peak of around 2 million tons in the late 1960s to less than a million tons in the 1990s. This decline has been due, mainly, to a collapse in the sardine stock in the late 1960s and 1970s, and a reduction in the catches of hake and horse mackerel under a conservative management strategy in the past decade. Changes in the abundance and distribution of commercially important species, as determined by acoustic and trawl surveys and catch-based analytical methods, are presented. The effect of major environmental anomalies on the distribution and abundance of the resources in recent years is discussed. The most dramatic anomaly in recent years was the wide-scale advection of low-oxygen water into the northern Benguela from the Angola Dome in 1994, and the subsequent Benguela Niño of 1995, which appear to have severely impacted the Namibian sardine population and many other resources. The present socio-economic value of the Namibian fishing industry is given together with the broad policy, legislation and formal structures for managing the living marine resources. 相似文献
3.
《African Journal of Marine Science》2013,35(1):131-144
An age-structured population model was used to examine the variability of yield created by randomly fluctuating recruitment success in the South African anchovy Engraulis capensis stock. Three different harvesting strategies were examined: (1) constant age-specific pattern of fishing mortality, (2) constant quota with effort limitation and (3) annual quota adjustments by means of an F msy procedure. Variable recruitment was generated by a stochastic stock-recruit relationship, and mean yields, mean spawner biomasses and probability distributions of yield were calculated at each exploitation level after 150 projections from the population structure of anchovy in 1981. Under conditions of constant fishing mortality, the variability of yield increased continuously as the exploitation rate was increased. The maximum average yield (MAY) is the stochastic equivalent of the maximum sustainable yield (MSY) of deterministic models, but an attempt to sustain this MAY by a constant quota resulted in a very high risk of stock collapse. An Fmsy policy based on pre-season adult biomass resulted in more variable yields than were obtained at the equivalent constant fishing mortality at age. Stability of yield therefore demanded the acceptance of lower average yields than could be attained in the long term by quota adjustment. It was considered that the South African purse-seine industry could not cope with the wide fluctuations of yield necessary to attain MAY. Specific management policies sufficiently robust to withstand both fluctuating recruitment success and the uncertainties in the parameters of working population models would be required. 相似文献
4.
《African Journal of Marine Science》2013,35(3-4):713-717
The Ilha dos Tigres of Angola is the only sandy island off the coast of the 2 000km-long Namib Desert and it remains the least known coastal wetland on a desert coast rich in shorebirds. Two surveys of the Baia dos Tigres region in 1999 and 2001 indicated a rich wetland bird diversity consisting of 25 species, with a total of 11 000 birds, at a density of 33 birds km?1 of beach. We established breeding by three species of seabirds — two cormorants and the great white pelican Pelecanus onocrotalus — and confirmed northward range extensions for two of these species. The region supports seven regionally threatened Red Data birds. Several threatened marine turtles occur at the nearby Cunene River mouth, and given the possibility that turtles and other Red Data birds may breed at Ilha dos Tigres, it is recommended that it should become an integral part of the Iona National Park on the adjacent mainland. 相似文献
5.
《African Journal of Marine Science》2013,35(3):541-564
This paper considers the scientific achievements of the Benguela Environment, Fisheries, Interaction and Training (BENEFIT) Programme as reflected in 29 internally evaluated BENEFIT research projects carried out between 1998 and 2007 when the programme ended, and draws a number of important lessons relevant to future regional marine research programmes in the Benguela from the manner in which the projects were initiated and executed. The founding of BENEFIT, its organisational structures, and the way in which the projects were planned, formulated and executed through these structures are outlined. The major findings and other achievements of the research on fish resources, the environment and the linkages between them are discussed under these headings, and in the case of projects on resources, in relation to key questions developed from guidelines in the BENEFIT Science Plan. Based largely on a final evaluation by the programme's International Scientific Advisory Panel, it is concluded that BENEFIT added materially to greater knowledge of the dynamics of key resources in the Benguela, markedly increased the extent of environmental monitoring in the region, and the capacity for future such monitoring, and added significant new insights into key physical, chemical and biological processes of relevance to stock dynamics. BENEFIT's most important achievement in a wider sense was the pioneering development, almost from scratch, of a regional marine research community which, inter alia, was crucial in the development of other regional marine research activities in the Benguela, such as the Benguela Current Large Marine Ecosystem (BCLME) Programme whose primary objective was the creation of a Benguela Current Commission. In this sense, the establishment of the Commission in 2007 to deal with transboundary management of the living resources of the Benguela Current and regional biodiversity issues can be regarded as the greatest single legacy of BENEFIT. 相似文献
6.
The diatom genus Pseudo-nitzschia blooms throughout New Zealand's coastal waters. More than a decade of monitoring data were analysed in this study and seasonality was a significant factor in the appearance of Pseudo-nitzschia blooms. Austral spring and summer were the main bloom periods, although there were regional differences. Between January 2000 and August 2011, 8.4% of 29,000 seawater samples analysed for Pseudo-nitzschia exceeded the voluntary trigger level for biotoxin testing (10×104 cells l?1), and these were analysed by fluorescent in situ hybridisation (FISH) assays. Pseudo-nitzschia fraudulenta and P. pseudodelicatissima (low domoic acid [DA] producers) were the dominant bloom formers throughout New Zealand (each contributing 25%) followed by the highly toxic P. australis (10%). Shellfish flesh testing for DA was triggered on 8477 occasions between 2000 and 2011, but no samples exceeded the regulatory limit (20 mg/kg); the maximum concentration was 13 mg/kg (Marlborough Sounds, mid-winter 2010). 相似文献
7.
The dinoflagellate Alexandrium minutum is often associated with harmful algal blooms (HABs). This species consists of many strains that differ in their ability to produce toxins but have similar morphology, making identification difficult. In this study, species-specific rRNA probes were designed for whole-cell fluorescence in situ hybridization (FISH) to distinguish A. minutum from two phylogenetic clades. We acquired the complete SSU to LSU rDNA sequences (GenBank accession numbers JF906989-JF906999) of 11 Alexandrium strains and used these to design rRNA targeted oligonucleotide probes. Three ribotype-specific probes, M-GC-1, M-PC-2, and M-PC-3, were designed. The former is specific for the GC clade ("Global clade") of A. minutum, the majority of which have been found non-toxic, and the latter two are specific for the PSP (paralytic shellfish poisoning)-producing PC clade ("Pacific clade"). The specificity of these three probes was confirmed by FISH. All cells in observed fields of view were fluorescently labeled when probes and target species were incubated under optimized FISH conditions. However, the accessibility of rRNA molecules in ribosomes varied among the probe binding positions. Thus, there was variation in the distribution of positive signals in labeled cells within nucleolus and cytosol (M-GC-1, M-PC-3), or just nucleolus (M-PC-2). Our results provide a methodological basis for studying the biogeography and population dynamics of A. minutum, and providing an early warning of toxic HABs. 相似文献
8.
《African Journal of Marine Science》2013,35(1):27-32
In South Africa, kelp gulls Larus dominicanus first breed when 4 years old. At least 50% of 4-year-olds breed; virtually all older birds breed annually. Most chicks leave natal colonies in the austral autumn and winter, when about 6 months old, and 60% of them may not return until aged 2 or 3 years. One 3-year-old showed signs of defending territory, but birds only attain full adult plumage when aged 4. The plumage of 3-year-olds is similar to that of adults, but the white spots on the outer primaries are less well developed and the secondaries and inner primaries have no white trailing edge. Chicks are dark, whereas birds aged 1 and 2 years gain increasing amounts of pale or white plumage on the face, neck, underparts and tail. 相似文献
9.
《African Journal of Marine Science》2013,35(1):81-110
The Cape commercial linefishery, established during the first half of the 19th century, currently consists of about 2 500 vessels (±20 000 crew), which land some 15 500 tons of fish each year. In spite of a long history, a lack of a long-term catch and effort data series has severely hindered the management of the fishery. This paper providcs commercial catch and effort data for three periods during the 20th century: 1897–1906, 1927–1931 and 1986–1998. Trends in catch per unit effort (cpue) were verified with additional data from the inshore trawl-fishery, from fishery-independent surveys and, where possible, stock assessment. According to a Linefish Management Protocol developed for the Linefishery, any stock demonstrating a historical reduction in cpue or catch contribution of more than 75% is to be regarded as overexploited. Evaluations based on present datasets support this arbitrarily determined reference point for cpue, but reveal that catch composition is a poor indicator of stock status and should be used with caution. In spite of technological advances such as the advent of combustion engines, nylon lines. echo-sounders, electronic navigational aids, onboard freezer facilities and larger vessels, declines in catch rate indicative of severe overexploitation (i.e. 75–99%) were observed for many important linefish species during the 20th century. Most of these were higher-trophic-Level species from the warm/temperate East Coast, several of which are also endemic. Life-history characteristics of the vulnerable species (i.e those demonstrating declines in cpue of >75%) include predictable location in time and space (either coastal migrant or resident), longevity (>15 years) and late maturity (relative to maximum age). Apart frorreduced productivity associated with stock depletion, other setbacks, such as ecosystem alteration, loss of genetic diversity and shor-term commercial extinction, are also anticipated. In order to rebuild depleted linefish stocks it is deemed essential to create additional marine reserves, dramatically improve enforcement, develop extensive public awareness programmes and substantially reduce commercial effort. 相似文献
10.
《African Journal of Marine Science》2013,35(1):297-298
The relationship between mass and length and the landed mass/whole mass conversion factors for Agulhas sole Austroglossus pectoralis were investigated and compared to the results of an earlier study. Earlier length/mass relationships overestimate the mass of small soles but underestimate that of large soles. The mass conversion factor of the present study also differed from the earlier results. 相似文献