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在鳗弧菌侵染下,利用SMART方法构建了青蛤的cDNA文库,并采用高通量测序方法和BLASTX比对筛选出ESTs及免疫相关因子的基因.结果表明,所构建的cDNA文库的库容量为1.12×106,插入片段均在500bp以上;大于100bp的有效ESTs序列1113条,平均长度725bp,拼接后获得一致性序列420条,其中包括126个叠联群和294个单拷贝EST,BLASTX比对后获得注释序列271条,进一步筛选获得青蛤免疫相关因子基因序列45条,为克隆其免疫防御相关因子的基因提供了重要的基础. 相似文献
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Penelope A. Luckens 《新西兰海洋与淡水研究杂志》2013,47(4):497-514
The breeding and settlement seasons of the intertidal barnacles Chamaesipho brunnea, C. columna and Elminius plicatus were determined, and experiments using cages on the shore to examine barnacle survival and growth rate at four levels were carried out at Leigh during 1964–66. Chamaesipho brunnea bred in spring and summer; C. columna and E. plicatus bred throughout the year, but settled intermittently. When protected from predators and from overgrowth by algae, all three species survived at levels lower than normal. At higher levels C. columna, E. modestus, Balanus trigonus and Tetraclita purpuraseens survived for significant periods (C. brunnea occurs naturally higher on the shore than the highest experimental level). 相似文献
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This study made use of data from three long-term fish tagging projects along the South African coastline to investigate the movement behaviour of the endemic black musselcracker Cymatoceps nasutus (Sparidae). From 1984 to 2016, a total of 3 430 C. nasutus (178–980 mm fork length) were tagged (with small plastic dart tags) throughout the species’ distributional range, with an overall reported recapture rate of 7.2%. Recaptured individuals displayed high levels of residency, moving an average of 14.8 km, with time-at-liberty ranging from 0 to 6 809 days. The majority of recaptures (84.6%) were made within 1 km of the tagging sites; however, some large-scale movements, of up to 528 km, were recorded. Although C. nasutus individuals moving greater distances were characterised by greater mean sizes (mm fork length) at time of recapture, the distances moved by juveniles were not significantly different from those of adults (p > 0.05). Low levels of connectivity among coastal regions were therefore expected due to the high degree of residency displayed by this species. Combining traditional management approaches together with a well-designed network of no-take marine protected areas is likely to be the most effective way to protect this vulnerable species. A lack of data on the movements of adults remains a challenge, but this could be overcome by tracking large individuals tagged with long-life acoustic transmitters. 相似文献
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Monitoring gravel augmentation in a large regulated river and implications for process‐based restoration 
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下载免费PDF全文 Fanny Arnaud Hervé Piégay David Béal Pierre Collery Lise Vaudor Anne‐Julia Rollet 《地球表面变化过程与地形》2017,42(13):2147-2166
The artificial gravel augmentation of river channels is increasingly being used to mitigate the adverse effects of river regulation and sediment starvation. A systematic framework for designing and assessing such gravel augmentations is still lacking, notably on large rivers. Monitoring is required to quantify the movement of augmented gravel, measure bedform changes, assess potential habitat enhancement, and reduce the uncertainty in sediment management. Here we present the results of an experiment conducted in the Rhine River (French and German border). In 2010, 23 000 m3 of sediments (approximately the mean annual bedload transport capacity) were supplied in a by‐passed reach downstream of the Kembs dam to test the feasibility of enhancing sediment transport and bedform changes. A 620‐m‐long and 12‐m‐wide gravel deposit was created 8 km downstream from the dam. Monitoring included topo‐bathymetric surveys, radio‐frequency particle tracking using passive integrated transponder (PIT) tags, bed grain size measurement, and airborne imagery. Six surveys performed since 2009 have been described (before and after gravel augmentation, and after Q2 and Q15 floods). The key findings are that (i) the augmented gravel was partially dispersed by the first flood event of December 2010 (Q1); (ii) PIT tags were found up to 3200 m downstream of the gravel augmentation site after four years, but the effects of gravel augmentation could not be clearly distinguished from the effects of floods and internal remobilization on more than 3500 m downstream; (iii) linear and log‐linear relationships linking bedload transport, particle mobility, and grain size were established; and (iv) combined bathymetry and PIT tag surveys were useful for evaluating potential environmental risks and the first morpho‐ecological responses. This confirmed the complementary nature of such techniques in the monitoring of gravel augmentation in large rivers. Copyright © 2017 John Wiley & Sons, Ltd. 相似文献