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The FG-9307 cell line derived from the gill of flounder Paralichthys olivaceus was used in the present study to determine the acute cytotoxic effects of the organophosphorous pesticide, profenofos. It was found that the cell growth rate was markedly reduced by profenofos at the concentrations of 2.5, 5 and 7.5 mg/L tested; and that the fine structures of the cells were also altered by profenofos, as evidenced by dilation of nuclear membranes and mitochondria cristae, and presence of enlarged lysoscrneswith engulfed organelles and numerous vacuoles in the cytoplama. Probably, mitochondria, the cell ener-gy-generating sites, are the most prcrninent sites of profenofos cytotoxity in the cells. This seems to be the first report of the use of marine fish cell line for evaluation of the acute in vitro cytotoxicity of organophos-phorus pesticide. 相似文献
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ＩＮＴＲＯＤＵＣＴＩＯＮＰｏｌｌｕｔｉｏｎｏｆｍａｒｉｎｅｅｃｏｓｙｓｔｅｍｐｏｓｅｓａｓｅｒｉｏｕｓｔｈｒｅａｔｔｏｍａｒｉｎｅｏｒｇａｎｉｓｍｓａｎｄｕｌｔｉｍａｔｅｌｙｔｈｅｅｎｔｉｒｅｅｃｏｓｙｓｔｅｍ.Ｉｎｒｅｃｅｎｔｙｅａｒｓ,ｏｒｇａｎｏｐｈｏｓｐｈｏｒｏｕｓ(ＯＰ)ｐｅｓｔｉｃｉｄｅｓｈａｖｅｌａｒｇｅｌｙｒｅｐｌａｃｅｄｏｒｇａｎｏｃｈｌｏｒｉｎｅｐｅｓｔｉｃｉｄｅｓｂｅｃａｕｓｅｏｆｔｈｅｉｒｒａｐｉｄｂｒｅａｋｄｏｗｎｉｎｗａｔｅｒａｎｄｔｈｅｉｒｌｏｗｅｎｖｉｒｏｎｍｅｎｔａ… 相似文献
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Dong Shugang 《中国海洋大学学报(自然科学版)》2000,30(Z1):207-214
实验研究了丙溴磷对蜈蚣藻的生 长、光合和呼吸作用以及叶绿素、类胡萝卜素、可溶性糖含量、电解质外渗率、过氧化物酶 、过氧化氢酶和硝酸还原酶活性的影响。结果表明：(1)低浓度(50)为3.7mg/L,对光合作用的 48hEC50为6.19mg/L,对呼吸作用的48hEC50为8.47mg/L。此外,高浓度的丙溴 磷还可引起藻体可溶性糖含量增加,叶绿素、类胡萝卜素含量降低,过氧化氢酶、过氧化物 酶活性降低,细胞膜损伤,电解质外渗率升高。(3)研究还发现藻体在受到丙溴磷伤害时其 硝酸还原酶活性急剧升高,最高可达到对照的几十倍,硝酸还原酶活性有可能成为指示藻体 受伤害程度的一项生化指标。 相似文献
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