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The effects of recombinant eel growth hormone (reGH).methyltestosterone (MT)and L-thyroxine (T4)on the growth of red sea bream. Pagrosomus major.were investigated.Administration of reGH to fry by immersion at 2 mg 1 for 2 hevery 5 dsys resulted in significant increase in both weight and length.but the condition factor (CF) diminished relative to that of similarly treated controls over the 37day treatment period.Immersion in 0.1 mg:1 T4 also resulted in significant increase in both weight and length and higher survival rate of test fry compared to the controls. Immersion in MT had less effect on growth and high-dose resulted in high mortality.In the second study.injection of 2 μg reGH(gwk)caused a significant increase in the specific growth rate (SGR) of test red sea bream fingerlings relative to that of the controls during the 4-week treatment period and maintained the increasing trend over the post-treatment period (weeks 4-6).Injection of MT at a dosage of 1μg (gwk) resulted in a significant in-creas 相似文献
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the effects of recombinant eel growth hormone (reGH), methyltestosterone (MT) and L-thyroxine (T4) on the growth of red sea
bream,Pagrosomus major, were investigated. Administration of reGH to fry by immersion at 2 mg/1 for 2 h every 5 days resulted in significant increase
in both weight and length, but the condition factor (CF) diminished relative to that of similarly treated controls over the
37 day treatment period. Immersion in 0.1 mg/l T4 also resulted in significant increase in both weight and length and higher
survival rate of test fry compared to the controls. Immersion in MT had less effect on growth and high-dose resulted in high
mortality. In the second study, injection of 2 μg reGH/(g·wk) caused a significant increase in the specific growth rate (SGR)
of test red sea bream fingerlings relative to that of the controls during the 4—week treatment period and maintained the increasing
trend over the post-treatment period (weeks 4–6). Injection of MT at a dosage of 1μg/(g·wk) resulted in a significant increase
in SGR during the 4—week treatment period. Intramuscular injection of 0.1 μg T4/(g·wk) also resulted in a significant increase
in weight. Injection of high-doses of MT and T4 inhibited growth and resulted in darkening skin, bulging eyes and thinning
body. In muscle chemical composition, the treated groups had no significant differences compared to the controls, but the
high-dose MT and T4 groups showed significant increases in lipid content. Potential practical methods for hormone application
in fish culture are discussed. 相似文献
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JIANG Jie LIN Hong FU Xiaoting LI Mingming 《中国海洋大学学报(英文版)》2005,4(3):248-251
The use of synthetic anabolic steroid methyltestosterone (MT) as growth promoter is prohibited in China. Validations of analytical methods for MT residue in food and the results obtained have become indispensable. The high performance liquid chromatography (HPLC) method for the detection of MT with liquid-liquid extraction by trichloromethanemethanol in carp muscle tissue was preliminarily validated with reference to the following parameters: recovery (accuracy) at the 1, 5 and 10 mgkg^-1 level, between-run and within-run CV values (repeatability, also called relative standard deviation (RSD)) and limit of detection. The recoveries were above 80% and the between-run and within-run CV values below 10% for muscle tissue. The limit of detection was 0.05 mgkg^-1. 相似文献
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本研究利用高压液相色谱法(HPLC)法测定:外源睾酮(T)和甲基睾酮(MT)(10μg/g)腹腔注射给入真鲷(Pagrosomusmajor)鱼体后,在血液中的代谢状况.证明,T和MT的吸收和清除很快。MT在注射1h达最大吸收峰(283ng/ml),24h内很快降低。T的吸收和清除状况与MT相似,不同的是其吸收值(最大值为64ng/ml)比MT低许多。随着鱼类养殖至商品规格,类固醇激素的含量将降至很低,因此,MT可做为一种生长促进因子用于水产养殖生产。 相似文献
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