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β诺达病毒利用其缺乏校正功能的聚合酶进行基因组复制, 易导致突变, 因而具有广泛的宿主感染性, 而且可引发致死率极高的病毒性神经坏死症, 需要有针对性地研究检测及防御方法。本研究以感染牙鲆(Paralichthys olivaceus)的β诺达病毒为对象, 利用引物设计, 将His标签编码序列连接到完整病毒衣壳蛋白C端, 构建原核表达载体; 采用SDS-PAGE及质谱对表达产物进行分离和鉴定; 重组衣壳蛋白经镍离子亲和柱纯化后进行复性条件的正交优化。结果表明4个肽段经质谱鉴
定与预期一致; 纯化产物产量可达36mg/L; 4°C条件下, 复性缓冲液中尿素和PBS的最适浓度为0.8mol/L和0.05mol/L。本研究建立的制备方案可为研制相关疫苗以及开发针对该病毒的快速检测产品等提供有价值的参考。 相似文献
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为更好理解燕山带的构造,作者在燕山地区开展了构造填图.在近年的构造填图中于辽宁省绥中县永安堡地区识别出大型的构造窗,其原始的逆掩推覆构造系统上盘由太古宇片麻岩及上覆岩系组成,而下盘由强烈韧性变形的张家口组火山岩系组成.填图结果表明,永安堡地区并不是一个张家口组岩层形成的宽缓向斜,也不是大型古火山机构.燕山地区在早白垩世张家口组火山岩喷发之后仍然存在强烈的区域挤压变形作用.基于野外观察和填图工作,我们认为国内已发表的地质图件未能真实反映燕山带的区域构造基本特征. 相似文献
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β-agarase AgaB appears to represent a new family of glycoside hydrolase; it is structurally and functionally different from other known agarases. In the present study, AgaB was expressed with a temperature-inducible expression system in E. coli BL21 (DE3) as a fusion protein bearing a C-terminal hexahistidine tag. The protein existed mainly in the form of inclusion body.After being washed and solubilized, AgaB in inclusion body was denatured and purified to electrophoretic purity by immobilized metal affinity chromatography. The purified AgaB was then refolded using a simple pulse dilution method, and the refolded AgaB showed a high specific hydrolysis activity of about 1600 units/mg protein. Forty milligrams of refolded pure protein were obtained from 1L of culture. 相似文献
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The northern fold belt away from the Singhbhum Shear Zone displays a set of folds on bedding. The folds are sub-horizontal
with E-W to ESE striking steep axial surfaces. In contrast, the folds in the Singhbhum Shear Zone developed on a mylonitic
foliation and have a reclined geometry with northerly trending axes. There is a transitional zone between the two, where the
bedding and the cleavage have become parallel by isoclinal folding and two sets of reclined folds have developed by deforming
the bedding—parallel cleavage. Southward from the northern fold belt the intensity of deformation increases, the folds become
tightened and overturned towards the south while the fold hinges are rotated from the sub-horizontal position to a down-dip
attitude. Recognition of the transitional zone and the identification of the overlapping character of deformation in the shear
zone and the northern belt enable the formulation of a bulk kinematic model for the area as a whole. 相似文献
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Several thrust faults have been mapped in theYanshan belt ,as the thrust structure was first iden-tified by Wong (1928) (Ji et al .,2005 ; Wang et al .,2005a ;Davis et al .,2001 ,1998 ; Yang et al .,2001 ;Zheng et al ., 2000 ; Chen, 1998 ; Zhang et al .,1986 ; He ,1957 ;Chern and Hsiung,1935) .Becausethe Archean basement was widely involved in thethrust systemof the Yanshan belt ,domestic popularrecognition emphasized the thick-skinned characteris-tics and li mited horizontal displacement… 相似文献
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For understanding the Mesozoic tectonics of Yanshan （燕山 ） belt, the authors took geological mapping in the belt. A large-scale thrust structure was identified in Yonganpu （永安堡） area. in the western part of Suizhong （绥中 ） County, Liaoning （辽宁 ） Province during our recent mapping in the Yanshan belt. The hanging wall of the thrust was composed of Archean gneiss and the overlying Early Cretaceous Zhangjiakou （张家口 ） Formation; meanwhile, the strongly ductile deformed volcanic rocks of Zhangjiakou Formation comprised the footwall in Yong＇anpu tectonic window. This discovery indicates the existence of strongly contractional deformation in the Yanshan belt after the eruption of Early Cretaceous Zhangjiakou volcanic rocks. On the basis of mapping and research, it is concluded that the published official geological maps have failed to identify the major structural features of the Yanshan belt. 相似文献
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β-agarase AgaB appears to represent a new family of glycoside hydrolase; it is structurally and functionally different from
other known agarases. In the present study, AgaB was expressed with a temperature-inducible expression system in E. coli BL21 (DE3) as a fusion protein bearing a C-terminal hexahistidine tag. The protein existed mainly in the form of inclusion
body. After being washed and solubilized, AgaB in inclusion body was denatured and purified to electrophoretic purity by immobilized
metal affinity chromatography. The purified AgaB was then refolded using a simple pulse dilution method, and the refolded
AgaB showed a high specific hydrolysis activity of about 1600 units /mg protein. Forty milligrams of refolded pure protein
were obtained from 1L of culture. 相似文献
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