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在真鲷Pagrosomusmajor三倍体诱导研究中，对染色体倍数的分析表明，受精卵受精5min后，在1．5℃冰水中处理5min，可获得17％的三倍体诱导率和28％的嵌合体比率，染色体数分别是：2N＝48，3N＝7Z，嵌合体50—68。仔鱼红血细胞测量和组织切片分析显示，三倍体和二倍体的红血细胞核直径（长径，下同）、视网膜神经节细胞核直径和中脑细胞核直径大小存在差异，其比率分别是1．42，1．35和1．41，这种差别必然导致三倍体鱼和二倍体鱼在代谢与功能方面的差异。应加强对鱼类三倍体形成机制及应用潜力的研究，同时注意开展其生理学、生化学和行为学的比较研究。 相似文献
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分别于1989年3—6月、1990年3—5月在天津和青岛两地采用冷、热休克法对黑鲷进行三倍体的人工诱导研究。结果表明,冷休克法诱导三倍体的效果好于热休克法,其最适诱导条件为卵子在受精后5min,温度为3—4℃的海水中处理10—20min,三倍体诱导率最高可达50.35％;正交试验证明,三倍体率受冷、热休克法的三因素影响程度的顺序由大到小依次为,处理时间、处理时刻、处理温度;黑鲷染色体数目:2n=48,3n=72,它们的染色体组型也同时获得。 相似文献
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用冷热休克诱导三倍体近江牡蛎 总被引：1，自引：0，他引：1
在近江牡蛎(Ostrea rivularis Gould)卵子授精后25分钟,分别用高温(35℃)和低温(10℃)持续处理20分钟,其胚胎发育初期的三倍体诱导率分别达到64％和69％。在这个高温点和低温点以外随着处理温度的升高或降低,三倍体诱导率均迅速减小。 相似文献
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Heat shocks effectively produced triploids inPenaeus chinensis. Fertilized eggs heat shocked (28–32°C) for 8 to 16 minutes, starting from 8 to 20 minutes after spawning, resulted in triploidy
induction rates of 39%–75%. Several triploid populations were cultured to 10 cm. In a triploid population, two kinds of ovaries
were observed. Histological examination showed apparent differences between these two kinds of ovaries, whereas among male
shrimp, there were no such differences.
Contribution No. 3465 from the Institute of Oceanology. Chinese Academy of Sciences.
The study was supported by the International Foundation for Science, Asian Fisheries Society and Chinese Climbing Project
PDB 6–2, and Chinese National 863 Project (No. 863-819-01-05). 相似文献
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Temperature treatment to inhibit extrusion of the polar body of the egg was first used on a lower crustacean,Artemia salina (Gross, 1932), and then was used for inducing triploids of amphibian (Frankhauser and Griffiths, 1939), fish (Svardson, 1945),
and mammal (Beatly and Fishchberg, 1949).
So far, induction of triploidy has been extensively used to obtain sterile or quick-growth individuals in fish (Swarup, 1956;
Lincoln, and Scott, 1983 and Thorgaard, 1986) and mollusk (Stanley et al., 1984), but similar work has not been reported on
crab, a higher crustancean. 相似文献
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AN EXPERIMENT ON INDUCTION OF TRIPLOIDY IN ERIOCHEIR JAPONICUS HEPUENSIS DAI BY HEAT TREATMENT 总被引：1，自引：0，他引：1
Temperature treatment to inhibit extrusion of the polar body of the egg was first used on a lower crustacean, Anemia salina (Gross, 1932), and then was used for inducing triploids of amphibian (Frankhauser and Griffiths, 1939), fish (Svardson, 1945), and mammal (Beatly and Fishchberg, 1949).So far, induction of triploidy has been extensively used to obtain sterile or quick-growth individuals in fish (Swarup, 1956; Lincoln.and Scott, 1983 and Thorgaard, 1986) and mollusk (Stanley et al., 1984), but similar work has not been reported on crab, a higher crustacean. 相似文献
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ＩＮＴＲＯＤＵＣＴＩＯＮＴｒｉｐｌｏｉｄｙｉｎｄｕｃｔｉｏｎａｎｄｇｙｎｏｇｅｎｅｔｉｃｄｉｐｌｏｉｄｉｎｄｕｃｔｉｏｎａｓｍａｉｎｐａｒｔｓｏｆｃｈｒｏｍｏｓｏｍａｌｍａｎｉｐｕｌａ ｔｉｏｎｈａｖｅｂｅｅｎｐｌａｙｉｎｇｍｏｒｅａｎｄｍｏｒｅｉｍｐｏｒｔａｎｔｒｏｌｅｓｉｎｍｏｄｅｒｎｆｉｓｈｂｒｅｅｄｉｎｇ .Ｍｏｓｔｔｒｉｐｌｏｉｄｆｉｓｈａｒｅｓｔｅｒｉｌｅａｎｄｔｈｅｉｒｒｅｄｕｃｅｄｇｏｎａｄａｌｄｅｖｅｌｏｐｍｅｎｔｒｅｓｕｌｔｓｉｎｉｍｐｒｏｖｅｍｅｎｔｏｆｇｒｏｗｔｈｒａｔｅａ… 相似文献
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采用冷休克抑制第二极体排出的方法诱导了半滑舌鳎三倍体,并探讨了三倍体诱导的最佳条件.通过流式细胞仪倍性检测和染色体观察验证各组三倍化率.结果显示:在受精后5 min开始,将受精卵放在4℃海水中进行冷休克处理,持续时间为20 min,三倍体诱导率最高为35％.通过对三倍体染色体进行分析,辨认其雌雄染色体型为ZWW/ZZZ,出现频率为1∶1,没有发现ZZW型雌鱼,暗示半滑舌鳎Z染色体和W染色体之间的交叉和互换受到严重抑制. 相似文献