2型糖尿病、非酒精性脂肪肝与血清胆碱酯酶的关系探讨     

周侠  游娜  徐家蓉  缪珩 《寒旱区科学》2017,9(5):140-145

目的 探讨2型糖尿病（T2DM）、非酒精性脂肪肝（NAFLD）与血清胆碱酯酶（S-CHE）的关系。方法 随机选取221例T2DM患者,将患者分为T2DM合并NAFLD、未合并NAFLD两组。并随机选取同一时期体检的工人及退休人员206例,分为单纯NAFLD和正常对照组。所有被检者进行餐前和餐后2h血糖、糖化血红蛋白（HbA1c）、肝肾功能、血脂、S-CHE的检测以及肝胆胰脾B超检查;对T2DM患者进行空腹C肽和餐后C肽的测定,并计算胰岛素抵抗指数（HOMA2-IR）、胰岛素敏感度指数（HOMA2-%S）、胰岛β细胞功能（HOMA2-%B）。结果 DM合并NAFLD和单纯NAFLD组的S-CHE水平明显高于T2DM未合并NAFLD组和正常对照组（P均<0.01）;随着T2DM病程的延长,S-CHE、ALT、GGT、TG、TC、空腹C肽、餐后C肽的水平逐渐下降,年龄逐渐增高（P<0.05或P<0.01）;将T2DM患者分为A（S-CHE<8900IU/L）、B（S-CHE≥8900IU/L）两组,B组患者的BMI、脂肪肝百分比、ALT、AST、GGT、TG、TC、LDL-C、HOMA2-IR高于A组,HOMA2-%S低于A组（P<0.05或P<0.01）;Pearson偏相关分析显示,校正年龄、性别、病程、HbA1c、FBG、收缩压、舒张压后,S-CHE与HOMA2-IR、BMI、TG、TC、LDL-C、ALT、GGT水平呈正相关,与HOMA2-%S呈负相关（P<0.05或P<0.01）;多元线性回归分析显示,S-CHE与TC、HOMA2-IR、ALT水平呈正相关（R²=0.281,P均<0.01）。结论 S-CHE的水平在NAFLD患者明显增高,而未合并NAFLD的T2DM患者S-CHE的水平并无增高,S-CHE水平升高主要与脂肪肝、胰岛素抵抗及血脂紊乱相关,常规检测S-CHE可以反映T2DM患者胰岛素抵抗的情况以及早期预测NAFLD的发生。  相似文献   

Cholinesterase activities in the Antarctic scallop Adamussium colbecki: Tissue expression and effect of ZnCl₂ exposure     

I. Corsi  S. Bonacci  G. Santovito  M. Chiantore  L. Castagnolo  S. Focardi 《Marine environmental research》2004,58(2-5):401

Biochemical characterization of cholinesterase activity (ChE) was carried out on the Antarctic scallop Adamussium colbecki collected in winter 2000 from Campo Icaro (Ross Sea, Antarctica) in order to increase its suitability as a sentinel organism for monitoring the Antarctic environment. The digestive gland, gills and adductor muscle were investigated for substrate specificity and inhibitors sensitivity using acetylthiocholine iodide (ASCh) and butyrylthiocholine iodide (BSCh) as substrates and tetra (monoisopropyl)pyrophosphor-tetramide (Iso-OMPA), 1,5-bis(4-allyldimethylammoniumphenyl)-penthan-3-one dibromide (BW284c51) and the insecticide chlorpyrifos as inhibitors. Effect of in vivo exposure to ZnCl₂ was also investigated. All the tissues expressed ChE activity (gill > adductor muscle > digestive gland) and low substrates specificity throughout the hydrolysis of both ASCh and BSCh substrates. Partial (25–29%) and total inhibition (100%) of ChE activity in gills was demonstrated following in vitro incubation with Iso-OMPA and BW284c51 (3 mM), respectively. Concentration-dependent inhibition was also evident with chlorpyrifos in the range 10⁻⁴–10⁻¹⁰ M (IC₅₀ 10⁻⁶) while in vivo exposure to ZnCl₂ did not seem to affect ChE activity in the scallop. The potential use of ChE in the A. colbecki as biomarker for monitoring water contamination in the marine Antarctic environment is discussed.  相似文献