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以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。 相似文献
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E. Schlecht U. Dickhöfer M. PredotovaA. Buerkert 《Journal of Arid Environments》2011,75(11):1136-1146
Goat husbandry in Oman’s Hajar Mountains supplies income and manure to farmers. An earlier analysis concluded that it uses purchased feeds inefficiently, but did not value the contribution of mountain pastures to goat nutrition and cropland fertilization. Therefore intake of pasture vegetation, cultivated forages and purchased feeds was determined in goats from three villages in spring and autumn 2005. Faecal excretion was quantified using TiO2 and diet digestibility was calculated from faecal nitrogen (N).Organic matter (OM) intake varied from 71 to 107 g kg−0.75 d−1. Pasture vegetation supplied 45-71% of OM intake, and at least 28%, 33% and 42% of phosphorus (P), metabolizable energy (ME) and N intake. While ME intake just covered maintenance and locomotion requirements, N and P intake exceeded the animals’ requirements. Therefore faecal concentrations (g kg−1 OM) of 26-36 g N and 4-8 g P were high, making goat dung a key element of sustainability for the local cropping systems.Since mountain pastures supply nutrients to goats and cropland, their long-term productivity is crucial. Feeding cultivated forages seemingly reduces intake on pasture, but a comprehensive evaluation of nutritional and economic implications of this strategy and possible alternatives is needed. 相似文献
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3种益生素配伍对异育银鲫(Carassius auratus gibelio)生长、消化及肠道茵群组成的影响 总被引：4，自引：1，他引：4
选用196尾1龄异育银鲫[体重为(30±2)g],随机分成4组,其中,1组为对照组,2、3、4为试验组,1组投喂的为基础饲料,2、3、4组投喂的饲料是在基础饲料中分别添加100μg/g地衣芽孢杆菌、100μg/g地衣芽孢杆菌与100μg/g低聚木糖、100μg/g地衣芽孢杆菌与300μg/g复合酶制剂,在室内流水养殖系统中饲养112天。研究3种益生素及其配伍对异育银鲫生长、消化及肠道菌群组成等的影响。结果表明,2、3、4组异育银鲫的增重率比1组(对照)均有提高,并分别比对照组提高了23.97%、43.78%和18.36%(P<0.05)。试验组的营养物质表观消化率、肠道酶活性及肝胰脏蛋白酶mRNA表达量与对照组相比均有提高且差异显著(P<0.05)。在肠道菌群组成上,各试验组大肠杆菌数和乳酸杆菌均比对照组少且差异显著(P<0.05);而在肠道芽孢杆菌方面,除芽孢杆菌与酶制剂配伍组外,其余两试验组对芽孢杆菌均有促生长作用,芽孢杆菌与寡糖配伍组对芽孢杆菌的促生长作用明显(P<0.05)。由此可见,芽孢杆菌、低聚木糖、复合酶制剂及它们的配伍物可以促进异育银鲫的生长,提高饲料利用率,促进肠道有益微生物的生长和抑制有害微生物,并且能提高肝胰脏蛋白酶mRNA表达量和肠道酶活性。 相似文献
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Edgardo D. Cerqueira Alicia M. Senz Celia M. Rabotnikof 《Journal of Arid Environments》2004,59(4):645-656
This study assessed the nutritive value of the most important forage species of the Calden forest (central semi-arid La Pampa, Argentina), for samples collected in fall, winter and spring, under grazing conditions and during two successive years, for ranges of good and fair conditions. The crude protein concentration (CP) of short-winter grasses (Piptochaetium napostaense, Poa ligularis, Stipa clarazii and Hordeum stenostachys) was about 10%. Mid-winter grasses (S. tenuissima and S. gynerioides) never reached 6% CP. Summer grasses (Digitaria californica and Trichloris crinita) ranged from 7% to 9% CP. In vitro dry matter digestibility (IVDMD) was similar among short-winter and summer grasses (40–50%). Mid-winter grasses had the lowest IVDMD for all seasons (<40%). Effects of sampling year and range condition on CP were consistently significant only for short-winter grasses. Good condition ranges provide a more acceptable forage supply than fair condition ranges. 相似文献
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以棉粕蛋白为酶解底物,用枯草杆菌蛋白酶对其进行酶解,以酶解产物1.5%和3.0%两个梯度等量替代鱼饲料配方中棉粕,在室内流水养殖系统中喂养异育银鲫鱼种[体重为(30±2)g]65天。测定鱼的生长、营养物质表观消化率、消化蛋白酶活性及肝胰脏中胰蛋白酶mRNA表达水平等指标。结果表明,添加1.5%和3.0%棉粕酶解产物的鱼在饲养35天后的特定增长率(SGR)分别比对照组高32.5%和56.7%,且差异显著(P<0.05);在饲养65天后,两组特定增长率分别比对照组高8.0%和21.0%,且差异极显著(P<0.01),肝胰脏中胰蛋白酶mRNA表达水平也随棉粕酶解产物添加梯度提高而相应提高,表明鱼的生长与消化蛋白酶mRNA表达水平相关。同时棉粕蛋白酶解物对肠道蛋白酶的活性和营养物质表观消化率都有促进作用,而棉粕蛋白酶解物对鱼肌肉成分并没有改变,这也表明棉粕蛋白酶解物在促进鱼生长、内源酶活性同时并未降低鱼的品质。 相似文献
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