排序方式： 共有5条查询结果，搜索用时 15 毫秒
1
1.
Jin Hai-zhu Guo Cheng-hua He Jun Wang Qing-hua Jiang Zhu-mao Sun Fu-zhang 《中国海洋湖沼学报》1998,16(4):364-368
The effect of Mg2+, NaCl and citric acid on the accumulation of β-carotene inDunaliella Salina was studied. The experimental results showed that 10.5 mmol/L Mg2+, 5 mol/L NaCl, 3 μmol/L citric acid, and CO2 are favorable forDunaliella Salina cell growth and β-carotene accumulation. After 144 h culture under the above conditions, theDunaliella Salina biomass increased by 7.18 times; β-carotene reached 9.61%.
Project 89023990 supported by the Shandong Natural Science Fund. 相似文献
2.
On the red coloration of saltern crystallizer ponds 总被引：3，自引：0，他引：3
To assess, respectively, the contribution of red bacteria of theHalobacterium-Haloferax-Haloarcula group and of the β-carotene-rich green algaDunaliella salina to the red colour of saltern crystallizer ponds, we studied the optical properties of the brines of NaCl-saturated saltern
ponds in Eilat, Israel, and quantified the pigments present in their biota. The absorption spectrum of the brines, as measured
using the opal glass method to reduce the interference by scattering, or using an integrating sphere, nearly corresponded
to thein vivo absorption spectrum of bacterioruberin-containing red archaeobacteria. However, extracts of the microbial community in organic
solvents were dominated by β-carotene, a pigment occurring in high concentrations in theDunaliella cells present, and bacterioruberin contributed only between 13 and 28 per cent of the total visible light absorption by the
extracts. The apparent discrepancy between these results can be explained by the very small in vivo optical cross-section
of β-carotene, which is densely packed in globules inside theD. salina cells. 相似文献
3.
Experimental determination of carotenoid degradation 总被引：1，自引：0，他引：1
P. R. Leavitt 《Journal of Paleolimnology》1988,1(3):215-227
4.
Dunaliella salina, a halotolerant unicellular green alga, can accumulate a large amount of β-carotene under certain environmental conditions.
The isomers of β-carotene extracted fromD. salina cultured in medium with different nitrate and phosphate concentrations were analysed by HPLC with Alox-T alumina column.
At least six isomers were found in different proportions depending on the culture media's nitrate and/or phosphate concentrations.
Nitrate and/or phosphate deficiency was conducive to the accumulation of total cis isomers but not of all trans isomer. It
is suggested that 1 mmol/L KNO3 and 0.1 mmol/L KH2 PO4 are favourable for accumulation of total cis β-carotene.
Contribution No. 2090 from the Institute of Oceanology, Chinese Academy of Sciences.
This project was supported by the National Natural Science Foundation of China, Grant No. 38970587. 相似文献
5.
On the red coloration of saltern crystallizer ponds. II. Additional evidence for the contribution of halobacterial pigments 总被引：1，自引：0，他引：1
In a previous paper we attempted to assess the contribution of red bacteria of theHalobacterium — Haloferax — Haloarcula group and of the -carotene-rich green algaDunaliella salina to the red colour of saltern crystallizer ponds. By means of light absorption measurements, we showed that bacterioruberin contained in the bacteria was mainly responsible for the colour of the brines, in spite of the fact that -carotene derived fromDunaliella was the pigment present in the greatest amount. This apparent discrepancy was explained by the very smallin vivo optical cross-section of -carotene, which is densely packed in globules inside theD. salina cells. We recently observed that the centrifugation technique used in the previous study to collect biomass from the ponds was unsuitable for this type of measurements, as a substantial part of theDunaliella cells present did not sediment upon centrifugation due to the low specific gravity caused by the high -carotene content. Therefore similar measurements were performed with biomass collected by filtration. Again,in vivo absorption spectra were dominated by the absorption peaks of bacterioruberin. The results reported here show that, in spite of the methodological problem associated with the earlier study, all views and conclusions expressed in our earlier paper retain their validity. 相似文献
1