| 养殖黄颡鱼(Pelteobagrus fulvidraco) 鮰爱德华氏菌(Edwardsiella ictaluri)的分离鉴定与生物学特性研究 |
| |
| 引用本文： | 耿 毅,汪开毓,范方玲,陈德芳,黄锦炉.养殖黄颡鱼(Pelteobagrus fulvidraco) 鮰爱德华氏菌(Edwardsiella ictaluri)的分离鉴定与生物学特性研究[J].海洋与湖沼,2010,41(1):61-67. |
| |
| 作者姓名： | 耿 毅 汪开毓 范方玲 陈德芳 黄锦炉 |
| |
| 作者单位： | 动物疫病与人类健康四川省重点实验室,四川农业大学,雅安,625014 |
| |
| 基金项目： | 教育部“长江学者和创新团队发展计划”创新团队项目资助,IRTO848号;;四川省教育厅重点项目资助,08ZA082号 |
| |
| 摘 要： | 从四川眉山与新津两地患红头病的养殖黄颡鱼体内分离到2株优势菌(CHNYC001与CHNYC002),以腹腔注射与浸泡的方式进行人工感染试验,证实其为养殖黄颡鱼红头病的病原菌。根据分离菌株的形态、生理生化特性,结合16SrDNA序列测定(GenBank登录号分别为FJ766524、FJ766525)与系统发育分析,将其鉴定为鮰爱德华氏菌(Edwardsie lla ictaluri)。分离菌株最适生长温度为20—30℃,最适生长pH为7.0,在含盐量2%以上的培养基上不生长,对氟苯尼考、强力霉素、洛美沙星等敏感,对乙酰螺旋霉素、磺胺甲基异噁唑和麦迪霉素等不敏感。临床病理特征分析发现,养殖黄颡鱼感染爱德华氏菌临床上分为急性与慢性两种类型,急性型主要表现为败血症的病变特征,慢性型表现为头顶出血,发红与溃疡的病变特征。
|
| 关 键 词： | 黄颡鱼 红头病 爱德华氏菌 分离鉴定 生物学特性 |
| 收稿时间： | 2008/12/27 0:00:00 |
| 修稿时间： | 2009/3/10 0:00:00 |
ISOLATION, IDENTIFICATION AND BIOLOGICAL CHARACTERIZATION OF EDWARDSIELLA ICTALURI FROM CULTURED YELLOW CATFISH PELTEOBAGRUS FULVIDRACO |
| |
| GENG Yi,WANG Kai-Yu,FAN Fang-Ling,CHEN De-Fang and HUANG Jin-Lu.ISOLATION, IDENTIFICATION AND BIOLOGICAL CHARACTERIZATION OF EDWARDSIELLA ICTALURI FROM CULTURED YELLOW CATFISH PELTEOBAGRUS FULVIDRACO[J].Oceanologia Et Limnologia Sinica,2010,41(1):61-67. |
| |
| Authors: | GENG Yi WANG Kai-Yu FAN Fang-Ling CHEN De-Fang and HUANG Jin-Lu |
| |
| Institution: | Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University;Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University |
| |
| Abstract: | Bacterial strains CHNYC001 and CHNYC002 were isolated from cultured yellow catfish Pelteobagrus fulvidraco with red-head disease in Meishan and Xinjin counties, Sichuan Province. The strains were proved to be the pathogens of the disease in our challenging experiments by immersion and intraperitoneal injection. The isolated strains were identified as Edwardsiella ctaluri in morphology, physiology and biochemistry, as well as 16S rDNA sequence (Ac- cessible Number in GenBank: FJ766524, FJ766525). The optimum... |
| |
| Keywords: | Pelteobagrus fulvidraco Red-head disease Edwardsiella ictaluri Isolation Identification Bio- logical characteristics |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《海洋与湖沼》浏览原始摘要信息 |
| 点击此处可从《海洋与湖沼》下载免费的PDF全文 |
|
