|
|||||
|
|
| 不同方法制备的海星皂甙细胞毒性评价 | |
| 引用本文： | 文震,党志,宗敏华,赵金华,李靖.不同方法制备的海星皂甙细胞毒性评价[J].海洋通报,2006,25(6):26-29,46. |
| 作者姓名： | 文震 党志 宗敏华 赵金华 李靖 |
| 作者单位： | 1. 华南理工大学生物科学与工程学院轻工技术与工程博士后流动站,广东,广州,510640;深圳企业博士后工作站海王分站,广东,深圳,518054 2. 华南理工大学环境科学与工程学院,广东,广州,510640 3. 华南理工大学生物科学与工程学院轻工技术与工程博士后流动站,广东,广州,510640 4. 深圳企业博士后工作站海王分站,广东,深圳,518054 |
| 基金项目： | 中国博士后科学基金;广东省自然科学基金 |
| 摘 要： | 考察超临界CO2萃取、乙醇萃取两种方法制备的南海产多棘海星皂甙的溶血过程,采用分光光度法测定溶血指数,结合红细胞形态血观察,评价其细胞毒性。结果显示,两种方法制备的海星皂甙都具有明显溶血作用,超临界CO2萃取、溶剂萃取的海星皂甙溶血指数分别为19231,12821,超临界CO2萃取的海星皂甙保持了更强的细胞毒性,这为海星皂甙制备工艺的选择,探索其物质基础提供了参考依据。 |
| 关 键 词： | 海星 皂甙 溶血 超临界 |
| 文章编号： | 1001-6392（2006）06-0026-04 |
| 收稿时间： | 2005-09-30 |
| 修稿时间： | 2005-09-302005-12-26 |
Cell Toxicity of Two Saponins Extracted by Different Methods from Starfish (Asterias amurensis) |
|
| WEN Zhen,DANG Zhi,ZONG Minhua,ZHAO Jinhua,LI Jing.Cell Toxicity of Two Saponins Extracted by Different Methods from Starfish (Asterias amurensis)[J].Marine Science Bulletin,2006,25(6):26-29,46. | |
| Authors: | WEN Zhen DANG Zhi ZONG Minhua ZHAO Jinhua LI Jing |
| Institution: | 1. Postdoctoral Station of Light Industry Technology and Engineering, College of Biological Science and Engineering, South China Univ, of Tech,, Guangzhou 510640, Guangdong, China; 2. Postdoctoral Scientific Research Station of Shenzhen, Neptunus Corporation branch, Shenzhen 518054, Guangdong, China; 3. College of Environmental Science and Engineering, South China Univ. of Tech., Guangzhou 510640, Guangdong, China |
| Abstract: | According to the hemolytic process, the cell toxicity of two saponins extracted by supercritical CO2 and organic solvent from Asterias amurensis in the South China sea was evaluated. The result showed all the saponins prepared by different methods had hemolysis to the red blood cell. Hemolytic activity showed that these saponins destroyed the membrane of the red blood cell The hemolytic index of two saponins extracted by supercritical CO2 and organic solvent were 19231 and 12821 respectively. The result provides reference for the selection extraction technique and material basis for toxicity. |
| Keywords: | saponin starfish cell toxicity hemolysis supercritical |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |