| 五种海洋鱼类消化道G细胞的定位 |
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| 引用本文： | 韩师昭,朱艾嘉,叶海辉,黄辉洋.五种海洋鱼类消化道G细胞的定位[J].海洋科学,2008,32(1):52-55. |
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| 作者姓名： | 韩师昭 朱艾嘉 叶海辉 黄辉洋 |
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| 作者单位： | 宁波海洋环境监测中心,浙江,宁波,315040 |
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| 基金项目： | 国家自然科学基金
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福建省自然科学基金 |
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| 摘 要： | 应用胃泌素多克隆抗体和链霉菌抗生物素蛋白-过氧化物酶免疫组织化学方法(SP法),对中华乌塘鳢(Bostrichthys sinensis)、黄鳍鲷(Sparus latus)、牙鲆(Paralichthys olivaceus)、褐篮子鱼(Sigauns fusces-ens)和大弹涂鱼(Boleophthalmus pectinirostris)消化道胃泌素细胞(G细胞)进行免疫组化鉴别和定位。结果显示:中华乌塘鳢G细胞主要分布在小肠,直肠偶见。黄鳍鲷G细胞仅在小肠发现。牙鲆G细胞大量位于幽门胃和小肠。褐篮子鱼G细胞在幽门盲囊和小肠有少量分布。大弹涂鱼G细胞在贲门胃、幽门胃和小肠均有出现,大量分布于幽门胃。5种鱼类G细胞主要出现在胃和小肠的分布状况,与胃泌素调节胃肠消化吸收的功能相一致。5种鱼类G细胞形态类型多样,提示了胃泌素以不同的内分泌方式参与消化生理过程。
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| 关 键 词： | 海洋鱼类 消化道 胃泌素细胞 免疫组织化学 |
| 文章编号： | 1000-3096（2008）01-0052-04 |
| 收稿时间： | 2004-10-08 |
| 修稿时间： | 2005-09-08 |
The localization of G cell in the digestive tract in five kinds of marine fish |
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| LI Jin-duo.The localization of G cell in the digestive tract in five kinds of marine fish[J].Marine Sciences,2008,32(1):52-55. |
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| Authors: | LI Jin-duo |
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| Abstract: | By using the gastrin antiserum and the immunohistochemical method of Streotavidin-Peroxid(S-P),the investigation on the distributions of G cells in the digestive tract of Bostrichthys sinensis,Sparus latus,Paralichthys olivaceus,Sigauns fuscesens and Boleophthalmus pectinirostris was made.The distributions of the G cells are as follows: In Bostrichthys sinensis,the G cells are mainly located in intestine,rarely in rectum;In Sparus latus,the G cells were only observed in intestine,none in any other parts of the digestive tract;In Paralichthys olivaceus,the G cells were observed numerously in stamachus pyloricus and intestine;In Sigauns fuscesens,the G cells were distributed in pyloric caecum and intestine;In Boleophthalmus pectinirostris,the G cells were observed in stomachus carsiacus,stamachus pyloricus and intestine,mainly located in stamachus pyloricus.The G cells were detected mainly in the stomach and intestine in 5 kinds of marine fish,the results show that the gastrin was correlated with function of digestion and absorption.The G cells were of polymorphism in 5 kinds of marine fish.It was suggested that the gastrin might exert the digestive physiology in different modes of endocrine. |
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| Keywords: | mrine fish digestive tract gastrin cell immunohistochemistry |
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