| 气相色谱质谱联用及电喷雾质谱法分析多棘海盘车的脑苷脂结构 |
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| 引用本文： | 丛培旭,李兆杰,徐杰,于槚槚,王玉明,薛长湖.气相色谱质谱联用及电喷雾质谱法分析多棘海盘车的脑苷脂结构[J].中国海洋大学学报(自然科学版),2011,41(12):63-68. |
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| 作者姓名： | 丛培旭 李兆杰 徐杰 于槚槚 王玉明 薛长湖 |
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| 作者单位： | 中国海洋大学食品科学与工程学院,山东青岛,266003 |
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| 基金项目： | 海洋公益性行业科研专项(201105029); 国际科技合作项目(2010DFA31330); “泰山学者”建设工程专项资助 |
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| 摘 要： | 通过硅胶柱层析法分离得到多棘海盘车脑苷脂,并采用气相色谱质谱联用(GC-MS)及电喷雾质谱(ESI-MS)分析鉴定其结构。GC-MS分析表明,多棘海盘车脑苷脂AAC-1、AAC-2的糖基组成相同,均为葡萄糖;长链碱主要由d18∶2、d18∶3以及少量的d22∶1组成;两者结构最主要的差别在脂肪酸部分,AAC-1由C2位不含羟基的脂肪酸组成,而AAC-2则由C2位含1个羟基取代的脂肪酸组成。ESI-MS分析结果表明,AAC-1和AAC-2的分子量范围在740～850amu之间。通过GC-MS和ESI-MS结合使用,可快速推测出多棘海盘车脑苷脂基本结构及单体分子组成,对下一步的构效关系研究具有重要意义。
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| 关 键 词： | 脑苷脂 气相色谱质谱联用 电喷雾质谱 长链碱 多棘海盘车 |
Structure Analysis of Cerebrosides in Starfish (Asterias amurensis) by GC-MS and ESI-MS |
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| CONG Pei-Xu,LI Zhao-Jie,XU Jie,YUJia-Jia,WANG Yu-Ming,XUE Chang-Hu.Structure Analysis of Cerebrosides in Starfish (Asterias amurensis) by GC-MS and ESI-MS[J].Periodical of Ocean University of China,2011,41(12):63-68. |
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| Authors: | CONG Pei-Xu LI Zhao-Jie XU Jie YUJia-Jia WANG Yu-Ming XUE Chang-Hu |
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| Institution: | CONG Pei-Xu,LI Zhao-Jie,XU Jie,YU Jia-Jia,WANG Yu-Ming,XUE Chang-Hu(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China) |
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| Abstract: | To determine the structure of the cerebrosides in the starfish Asterias amurensis.The cerebrosides isolated from Asterias amurensis through a silica gel column were further analyzed by Gas Chromatography-Mass Spectrometry(GC-MS) and electrospray ionization mass spectrometry(ESI-MS).The GC-MS rusults suggested that the LCBs and glycoside compositions of AAC-1 and AAC-2 are similar but with an obvious difference in their fatty acid composition.Their glycoside composition is glucose while the major LCBs are d1... |
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| Keywords: | cerebroside GC-MS ESI-MS long-chain base Asterias amurensis |
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