| 哈维氏弧菌VIB645毒性相关质粒的结构解析 |
| |
| 引用本文： | 侯晓阳,孙铂光,刘吉文,孙婧,张晓华.哈维氏弧菌VIB645毒性相关质粒的结构解析[J].中国海洋大学学报(自然科学版),2011,41(9):48-52. |
| |
| 作者姓名： | 侯晓阳 孙铂光 刘吉文 孙婧 张晓华 |
| |
| 作者单位： | 中国海洋大学海洋生命学院,山东青岛,266003 |
| |
| 基金项目： | 教育部科学技术研究重点项目(108082); 国家自然科学基金项目(30771656)资助 |
| |
| 摘 要： | 哈维氏弧菌(Vibrio harveyi)是水产养殖动物的常见致病菌。前期研究发现,哈维氏弧菌致病性最强的菌株VIB645含有2个核苷酸序列非常相似的溶血素基因—vhhA和vhhB,分别位于染色体DNA和1个大小约为65.6 kb的质粒上(命名为pVH1)。本研究用碱裂解法提取该菌株的毒性相关质粒pVH1,利用多种限制性内切酶进行酶切,构建随机DNA文库并测序。合计测序97.63 kb,覆盖度为148.3%,鉴定出125个ORF(开放阅读框,>150 bp)。这些预测的ORF,根据其功能主要分为以下几类:(1)毒力相关基因,包括RTX毒素钙离子结合蛋白、转铁蛋白结合蛋白A前体、外膜粘附蛋白;(2)结合转运相关基因,包括TraC、-D-、F-、G-、H-、I-、K-、N-、U-、W、TrbB-、C;(3)转座相关基因;(4)其他功能基因,如甲基转移酶,DNA结合蛋白,DNA复制蛋白DnaC等;(5)未知基因。本研究结果对揭示哈维氏弧菌的致病机理具有重要意义。
|
| 关 键 词： | 哈维氏弧菌 毒性相关质粒 结构解析 |
Random Sequencing and Sequence Analysis of the Virulence-Related Plasmid pVH1 from Vibrio harveyi VIB645 |
| |
| HOU Xiao-Yang,SUN Bo-Guang,LIU Ji-Wen,SUN Jing,ZHANG Xiao-Hua.Random Sequencing and Sequence Analysis of the Virulence-Related Plasmid pVH1 from Vibrio harveyi VIB645[J].Periodical of Ocean University of China,2011,41(9):48-52. |
| |
| Authors: | HOU Xiao-Yang SUN Bo-Guang LIU Ji-Wen SUN Jing ZHANG Xiao-Hua |
| |
| Institution: | HOU Xiao-Yang,SUN Bo-Guang,LIU Ji-Wen,SUN Jing,ZHANG Xiao-Hua(College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China) |
| |
| Abstract: | Vibrio harveyi has emerged as a serious pathogen of aquaculture.Previous studies revealed that the most pathogenic isolate,VIB 645,contained two closely related hemolysin genes(designated vhhA and vhhB).These two genes proved to be located in the genome and a plasmid(named pVH1) which is 65.6 kb in size.In this study,alkaline lysis method was used to extract the virulence-related plasmid pVH1 from Vibrio harveyi VIB645.The plasmid was digested by several restriction enzymes and sequenced by constructing ran... |
| |
| Keywords: | Vibrio harveyi virulence related plasmid sequence analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
