| Cultural characteristics of chromium resistant unicellular cyanobacteria isolated from local environment in Pakistan |
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| 作者姓名： | Abdul Hameed Shahida Hasnain |
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| 作者单位： | Department of Botany, University of the Punjab, Quaid-e-Azam Campus, Lahore-54590, Pakistan |
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| 基金项目： | Financially support by the Ministry of Science and Technology, Pakistan.ACKN0WLEDGMENTS The Ministry of Science and Technology of Pakistan is acknowledged for the financial funding of this work, a part of the project“Screening of Chromium Resistant bacteria/Cyanobacteria for Detoxification Potential and Plant Growth Stimulation under Chro- mium Stresses”. |
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| 摘 要： | Many unicellular cyanobacteria were isolated from different places： fields, ponds, polluted water, and soils from Muredkey and Kasur tannery areas, near Lahore, Pakistan. Different media like BG 11 medium, Bold Basal medium, Chu＇s # 10 medium and Gotham＇s medium, in standard forms and with slight variations of ingredients, and different pH, temperature and light regimes were checked for the optimum growth of the isolates. The isolation pro- cedure was repeated with different concentrations of chromium to select the resistant strains. These selected strains grew on chromium of the range 100-200 μg/ml in BG 11 medium. Cyanobacteria were maintained in solid and liquid media with/without shaking. Cyanobacterial strains were collected from natural habitats that were accompanied by a diversified group of organisms including bacteria, protozoan, and rotifers etc. In order to eliminate these agents termed as contaminants, we used several methods including phenol treatment, use of antibiotic and careful manual picking of unicellular cyanobacteria. Resistance of these strains against different heavy metals （ZnSO4, MnSO4, NiSO4, COCl2, Pb（NO3）3, CuSO4, HgCl2, AgNO3 and CdCl2） and antibiotics （erythromycin, streptomycin, kanamycin, chloramphenicol, neomycin） was evaluated. Optimum temperature was 30℃ with variable pH for the reduction of Cr^6＋ in to Cr^3＋ in majority of strains.
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| 关 键 词： | 巴基斯坦 铬 重金属 环境污染 生态系统 |
| 收稿时间： | 2004-08-19 |
| 修稿时间： | 2005-03-11 |
Cultural characteristics of chromium resistant unicellular cyanobacteria isolated from local environment in Pakistan |
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| Abdul Hameed Shahida Hasnain.Cultural characteristics of chromium resistant unicellular cyanobacteria isolated from local environment in Pakistan[J].Chinese Journal of Oceanology and Limnology,2005,23(4):433-441. |
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| Authors: | Abdul Hameed Shahida Hasnain |
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| Institution: | (1) Department of Botany, University of the Punjab, Quaid-e-Azam Campus, 54590 Lahore, Pakistan |
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| Abstract: | Many unicellular cyanobacteria were isolated from different places: fields, ponds, polluted water, and soils from Muredkey
and Kasur tannery areas, near Lahore, Pakistan. Different media like BG 11 medium, Bold Basal medium, Chu's #10 medium and
Gorham's medium, in standard forms and with slight variations of ingredients, and different pH, temperature and light regimes
were checked for the optimum growth of the isolates. The isolation procedure was repeated with different concentrations of
chromium to select the resistant strains. These selected strains grew on chromium of the range 100–200 μg/ml in BG 11 medium.
Cyanobacteria were maintained in solid and liquid media with/without shaking. Cyanobacterial strains were collected from natural
habitats that were accompanied by a diversified group of organisms including bacteria, protozoan, and rotifers etc. In order
to eliminate these agents termed as contaminants, we used several methods including phenol treatment, use of antibiotic and
careful manual picking of unicellular cyanobacteria. Resistance of these strains against different heavy metals (ZnSO4, MnSO4, NiSO4, CoCl2, Pb(NO3)3, CuSO4, HgCl2, AgNO3 and CdCl2) and antibiotics (erythromycin, streptomycin, kanamyci chloramphenicol, neomycin) was evaluated. Optimum temperature was
30°C with variable pH for the reduction of Cr6+ in to Cr3+ in majority of strains.
Financially support by the Ministry of Science and Technology, Pakistan. |
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| Keywords: | cyanobacteria Cr 6+ reduction heavy metals |
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