16S rRNA gene phylogenesis of culturable predominant bacteria from diseased Apostichopus japonicus (Holothuroidea, Echinodermata) |
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| Authors: | Haiyan Ma Guoliang Jiang Zhiqiang Wu Xin Wang |
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| Institution: | (1) College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, P. R. China |
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| Abstract: | Cultured Apostichopus japonicus in China suffers from a kind of skin ulceration disease that has caused severe economic loss in recent years. The disease,
pathogens of which are supposed to be bacteria by most researchers, is highly infectious and can often cause all individuals
in the same culture pool to die in a very short time. The 16S rRNA gene phylogenesis of the culturable bacteria from the lesions
of diseased individuals was conducted to study the biodiversity of the bacterial communities in the lesions and to identify
probable pathogen(s) associated with this kind of disease. S. japonica samples were selected from a hatchery located in the eastern part of Qingdao, China. Bacterial universal primers GM5F and
DS907R were used to amplify the 16S rRNA gene of bacteria colonies, and touchdown PCR was performed to amplify the target
sequences. The results suggest that γ- proteobacteria (Alteromonadales and Vibrionales) of CFB group, many strains of which have been also determined as pathogens in other marine
species, are the predominant bacterial genera of the diseased Apostichopus japonicus individuals. |
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| Keywords: | Apostichopus japonicus 16S rRNA gene phylogenesis bacteria skin ulceration disease |
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