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| 胡子鲇3个养殖群体遗传结构的AFLP分析 | |
| 引用本文： | 邓思平,张艳艳,朱春华,吴天利,师尚丽,张中国,赵志勇,李广丽.胡子鲇3个养殖群体遗传结构的AFLP分析[J].广东海洋大学学报,2012(3):24-28. |
| 作者姓名： | 邓思平 张艳艳 朱春华 吴天利 师尚丽 张中国 赵志勇 李广丽 |
| 作者单位： | 1. 广东海洋大学水产学院,广东湛江524025 2. 南海水产经济动物增养殖广东普通高校重点实验室,广东海洋大学,广东湛江5240252 3. 广西玉林市牧源渔牧有限公司,广西玉林537000 |
| 基金项目： | 广西科学研究与技术开发计划重点项目(桂科合1140009-4) |
| 摘 要： | 应用AFLP方法,从64对引物中筛选出5对用于分析胡子鲇(Claris fuscus)厦门(XM)、江门(JM)、玉林(YL)等3个养殖群体共90个个体群体内、群体间遗传多样性。扩增共得到289条清晰谱带,引物多态性介于47.8%～63.8%,平均多态性比率为56.4%。胡子鲇群体总遗传杂合度(Ht)、遗传分化系数(Hs)、遗传分化度(Gst)和基因流(Nm)分别为0.161 1、0.153 6、0.051 1和8.501 8,表明胡子鲇多态性处于中等偏下水平,群体内部遗传分化系数较高,三个群体间无较大分化,群体间基因流动较好。其中,JM群体多态性比率、有效等位基因数Ne、Nei’s指数和Shannon指数都较XM群体和YL群体高,种内分化程度较好。对群体进行两两比较发现,XM群体和YL群体间分化程度最大,XM群体和JM群体次之,JM群体和YL群体最小。基于杂交优势,推断XM和YL群体繁殖所产生的后代将具有比亲代更为优良的性状。 |
| 关 键 词： | 胡子鲇 群体结构 遗传多样性 AFLP |
Analysis on Genetic Structure of Three Cultured Populations Claris fuscu with AFLP Markers |
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| DENG Si-ping,ZHANG Yan-yan,ZHU Chun-hua,WU Tian-li,SHI Shang-li,ZHANG Zhong-guo,ZHAO Zhi-yong,LI Guang-li.Analysis on Genetic Structure of Three Cultured Populations Claris fuscu with AFLP Markers[J].Journal of Zhanjiang Ocean University,2012(3):24-28. | |
| Authors: | DENG Si-ping ZHANG Yan-yan ZHU Chun-hua WU Tian-li SHI Shang-li ZHANG Zhong-guo ZHAO Zhi-yong LI Guang-li |
| Institution: | 1(1.Fisheries College,Guangdong Ocean University,Zhanjiang 524025,China;2.Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes,Guangdong Ocean University,Zhanjiang 524025,China;3.Guangxi Yulin Source of Fish Animal Husbandry Co.,LTD,Yulin 537000,China) |
| Abstract: | Genetic diversities of 90 individuals(30 each population) from three cultured populations of Xiamen(XM),Jiangmen(JM) and Yulin(YL) were analyzed.5 from 64 primer-combinations displayed a total of 289 AFLP loci.The percentage of polymorphic loci was from 47.8% to 63.8%,and averaged 56.4%.The average total genetic diversity(Ht),the average genetic diversity within populations(Hs),the gene differentiation coefficient(Gst),and the gene flow index(Nm) was 0.161 1,0.153 6,0.051 1 and 8.501 8,respectively.Results showed that the gene diversity index of C.fuscus was in lower middle level,and the genetic variation mainly came from individuals within populations but not between geographic populations.Compared with the population XM and YL,population JM displayed better polymorphic,higher effective number of alleles,Nei’s gene diversity and Shannon genetic diversity index.Among the 3 cultured populations,the largest differentiation was displayed between population XM and YL,then between XM and JM,and the smallest between JM and YL.Results suggested that hybridization of population XM and YL could produce descendants with better characters. |
| Keywords: | Claris fuscu population genetic diversity AFLP |
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