| Isolation and characterization of phenanthrene-degrading strain Sphingomonas sp. GY2B |
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| 作者姓名： | Xueqin TAO Guining LU Zhi DANG Chen YANG Xiaoyun YI |
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| 作者单位： | College of Environmental Science and Engineering, South China University of Technology, Guangzhou 510640, China |
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| 摘 要： | Polycyclic aromatic hydrocarbons （PAHs） are of great concern since most of them have been shown to be toxic, mutagenic, and carcinogenic. Bioremediation, based on certain species of microorganisms, is regarded as an alternative method to detoxify or remove PAHs from the contaminated sites. Phenanthrene has been often used as a model substance for microbial metabolism of ＂bay-region＂ and ＂K-region＂ containing carcinogenic PAH. The focus of the present study is to isolate and identify microorganisms which could use phenanthrene as the sole carbon and energy source. Such microorganisms are expected to be of interest as a source organism for the bioremediation of PAHs polluted soils. The bacterial consortia were enriched from PAH contaminated soils with water-silicon oil biphasic systems. An aerobic bacterial strain efficiently utilizing phenanthrene as the sole carbon and energy source was isolated from bacterial consortia GY2 and identified as Sphingomonas sp. strain GY2B. Strain GY2B was susceptible to some common used antibiotics such as cefuroxime, chloramphenicol, erythromycin and tetracycline and intermediate susceptible to amoxycillin. Moreover, no plasmids could be isolated from strain GY2B using the standard alkaline-SDS method. At pH 7.4 and 30℃ strain GY2B had an optimal growth condition and in this condition above 99.8% phenanthrene was degraded under initial concentration of 100 mg/L. The addition of low concentration of glucose （10-200 mg/L） in MSM containing 100 mg/L phenanthrene could enhance the growth of strain GY2B, while the high concentration of glucose （〉500 mg/L） could exert an inhibitive effect on cell growth. Besides phenanthrene, strain GY2B could also use naphthalene, phenol, 1-hydroxy-2-naphthoic acid, 2-naphthol, salicylic acid and catechol as the sole carbon and energy source. The growth of strain GY2B on salicylic acid and catechol showed that the degradation of phenanthrene might follow salicylate pathway.
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| 关 键 词： | 菲 生物降解 PAHs 四环素 土壤污染 |
Isolation and characterization of phenanthrene-degrading strain Sphingomonas sp. GY2B |
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| Xueqin TAO Guining LU Zhi DANG Chen YANG Xiaoyun YI.Isolation and characterization of phenanthrene-degrading strain Sphingomonas sp. GY2B[J].Chinese Journal of Geochemistry,2006,25(B08):109-109. |
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| Abstract: | |
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| Keywords: | phenanthrene Sphingomonas sp biodegradation PAHs |
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