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Detection of the end point temperature of thermal denatured protein in fish and chicken meat through SDS-PAGE electrophoresis |
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| Authors: | Hongwei Gao Mao Mao Chengzhu Liang Chao Lin Jianhai Xiang |
| Institution: | 1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266003, P. R. China;Shandong Entry-Exit Inspection and Quarantine Bureau of P. R. China, Qingdao 266003, P. R. China 2. College of Food Science and Engineenng, Ocean University of China, Qingdao 266003, P.R.China 3. Shandong Entry-Exit Inspection and Quarantine Bureau of P. R. China, Qingdao 266003, P. R. China 4. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266003, P. R. China |
| Abstract: | Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was applied in the detection of the end point temperature (EPT) of thermal denatured protein in fish and meat in this study. It was also used in studying the thermal denatured temperature range of proteins in salmon and chicken meat. The results show that the temperature ranges of denatured proteins were from 65℃ to 75℃, and these temperature ranges were influenced by the processing methods. Through SDS-PAGE, the features of repeated heating thermal denatured proteins under the same temperature and processing time were studied. The electrophoresis pat-terns of thermal denatured proteins determined through repeated heating at the same temperature did not exhibit any change. For the detection of cooked fish and meat samples, they were subjected to applying the SDS-PAGE method, which revealed an EPT ranging from 60℃ to 80℃. |
| Keywords: | end point temperature detection SDS-PAGE electrophoresis |
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