The effect of supplementary dietary copper on copper and metallothionein levels in the fish, dab, Limanda limanda |
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| Authors: | J Overnell R McIntosh |
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| Abstract: | Common dab were fed a casein based diet containing copper at a concentration of 1·4 mg copper kg−1 (dry weight) or a similar diet supplemented with 200 mg copper kg−1. Fish were sampled after 10 weeks and 20 weeks and hepatic and renal copper, zinc and metallothionein concentrations were measured. All hepatic cytosolic copper in test and control fish co-eluted from Sephadex G-75 with metallothionein, as did some of the cytosolic zinc. After 20 weeks there was three times as much of this metallothionein-bound copper in the livers of the test fish compared to the control fish, although metallothionein concentration was little elevated. On the basis of these results it is proposed that cytosolic liver copper concentration or heat stable liver copper concentration can be used as an indication of excess dietary copper in the dab Limanda limanda. |
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