| 长牡蛎(Crassostrea gigas)中ChIP-seq 方法的建立 |
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| 引用本文： | 展望,王文,施威扬.长牡蛎(Crassostrea gigas)中ChIP-seq 方法的建立[J].海洋与湖沼,2015,46(6):1557-1563. |
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| 作者姓名： | 展望 王文 施威扬 |
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| 作者单位： | 同济大学生命科学与技术学院 上海 200092,同济大学生命科学与技术学院 上海 200092,同济大学生命科学与技术学院 上海 200092 |
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| 基金项目： | 上海市浦江人才计划项目, 14PJ1408400 号; 同济大学985 启动项目, 2000141505 号。 |
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| 摘 要： | 染色质免疫共沉淀技术(ChIP)作为研究DNA 结合蛋白与DNA 相互作用最有力的工具, 越 来越受到重视。但目前该技术在牡蛎等贝类中的应用尚未见相关报道。为了研究长牡蛎早期胚胎发 育过程中表观遗传信息的变化模式, 本文在长牡蛎胚胎中探索并建立了一套完整的染色质免疫共沉 淀方法, 构建了高质量的高通量测序文库, 首次获得了牡蛎全基因组水平的组蛋白H3K4me3 修饰分 布, 从而为研究牡蛎的表观遗传调控奠定了基础。
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| 关 键 词： | 长牡蛎(Crassostrea gigas) ChIP-seq 高通量测序 组蛋白修饰 |
| 收稿时间： | 2015/5/13 0:00:00 |
| 修稿时间： | 2015/8/19 0:00:00 |
DEVELOPMENT OF A ChIP-seq METHOD IN CRASSOSTREA GIGAS |
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| ZHAN Wang,WANG Wen and SHI Wei-Yang.DEVELOPMENT OF A ChIP-seq METHOD IN CRASSOSTREA GIGAS[J].Oceanologia Et Limnologia Sinica,2015,46(6):1557-1563. |
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| Authors: | ZHAN Wang WANG Wen and SHI Wei-Yang |
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| Institution: | School of Life Sciences and Technology, Tongji University, Shanghai 200092, China,School of Life Sciences and Technology, Tongji University, Shanghai 200092, China and School of Life Sciences and Technology, Tongji University, Shanghai 200092, China |
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| Abstract: | Chromatin immunoprecipitation coupled with high throughput sequencing (ChIP-seq) is a powerful tool to analyze DNA-protein interactions. However, the application of ChIP-seq in Crassostrea gigas has not been investigated. In this study, we established a protocol for performing ChIP-seq experiment in oyster early embryos. We successfully performed H3K4me3 ChIP-seq on 16-cell stage oyster embryo and revealed the genome-wide distribution of the H3K4me3 histone marks. This study is the first step toward understanding the epigenetic regulation of oyster embryogenesis. |
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| Keywords: | Crassostrea gigas ChIP-seq high-throughput sequencing histone modification |
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