Studies of the Biochemical Composition of Freshwater Plankton Part 2: The Amino Acids Contents in Green and Blue-Green Algae |
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| Authors: | B Havlíkík J Hanu sov J Hanu sov |
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| Institution: | B. Havlíkík,J. Hanu?sová,J. Hanu?sová |
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| Abstract: | The composition of bound amino acids was studied in selected types of green and blue-green plankton algae by means of a new elaborated method, using paper chromatography at the triple development of the chromatogram in the set-up: butanol: acetic acid: water at the rate of 4 : 1 : 5. Algologically and bacterially pure cultures of the green algae Ankistrodesmus falcatus, Haematococcus pluvialis, Chlorella pyrenoidosa, Pediastrum boryanum, Scendesmus obliquus and of the blue-green algae Anacystis nidulans, Microcystis pulverea and Microcystis aeruginosa were cultivated in inorganic and in organic medium. The contents of amino acids in green algae were qualitatively and quantitatively evaluated in relation to their physiologic condition (phase of logarithmic growth, phase of stagnation) and to the method of nutrition. The results were compared with the contents of amino acids in blue-green algae obtained in monocultures from freshwater at blue-green algae water blooms. The composition of bound amino acids was studied in selected types of green and blue-green plankton algae by means of a new elaborated method, using paper chromatography at the triple development of the chromatogram in the set-up: butanol: acetic acid: water at the rate of 4 : 1 : 5. Algologically and bacterially pure cultures of the green algae Ankistrodesmus falcatus, Haematococcus pluvialis, Chlorella pyrenoidosa, Pediastrum boryanum, Scendesmus obliquus and of the blue-green algae Anacystis nidulans, Microcystis pulverea and Microcystis aeruginosa were cultivated in inorganic and in organic medium. The contents of amino acids in green algae were qualitatively and quantitatively evaluated in relation to their physiologic condition (phase of logarithmic growth, phase of stagnation) and to the method of nutrition. The results were compared with the contents of amino acids in blue-green algae obtained in monocultures from freshwater at blue-green algae water blooms. The following amino acids were found in the organismus of primary production: alanine, aspartic acid, arginine, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, proline, serine and glycine, threonine, tryptophane, tyrosine and valine. The following amino acids were predominant from the quantitative aspect: glutamic acid, threonine and serine and glycine. The composition of amino acids in green algae cultivated in an inorganic medium did not differ significantly from cultures cultivated in an organic medium. Differences in the quantitative composition of the individual amino acids were ascertained with green algae in relation to their physiologic condition. |
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