| An AFLP Genetic Linkage Map of Pacific Abalone （Haliotis discus hannai） |
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| 作者姓名： | LI Qi XU Yanhong YU Ruihai KIJIMA Akihiro |
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| 作者单位： | [1]） College of Fisheries, Ocean University of China, Qingdao 266003, P. R. China [2]Marine Field Station, Field Science Center, Graduate School of Agricultural Science, Tohoku University, Onagawa, Oshika 986-2242, Japan |
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| 基金项目： | 教育部科学技术研究项目;国家自然科学基金 |
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| 摘 要： | A genetic linkage map of Pacific abalone （Haliotis discus hannai） was constructed using AFLP markers based on a two-way pseudo-testcross strategy in a full-sib family. With 33 primer combinations, a total of 455 markers （225 from the female parent and 230 from the male parent） segregated in a 1 ： 1 ratio, corresponding to DNA polymorphism： heterozygous in one parent and null in the other. The female framework map consisted of 174 markers distributed in 18 linkage groups, equivalent to the H. discus hannai haploid chromosome number, and spanning a total length of 2031.4 cM, with an average interval of 13.0 cM between adjacent markers. The male framework map consisted of 195 markers mapped on 19 linkage groups, spanning a total length of 2273.4cM, with an average spacing of 12.9cM between adjacent markers. The estimated coverage for the framework linkage maps was 81.2% for the female and 82.1% for the male, on the basis of two estimates of genome length. Fifty-two markers （11.4%） remained unlinked. The level of segregation distortion observed in this cross was 20.4%. These linkage maps will serve as a starting point for linkage studies in the Pacific abalone with potential application for marker-assisted selection in breeding programs.
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| 关 键 词： | 太平洋 鲍属 海洋生物 软体动物 |
| 收稿时间： | 23 November 2006 |
| 修稿时间： | 2006-11-232007-04-03 |
An AFLP genetic linkage map of pacific abalone (Haliotis discus hannai) |
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| LI Qi XU Yanhong YU Ruihai KIJIMA Akihiro.An AFLP genetic linkage map of pacific abalone (Haliotis discus hannai)[J].Journal of Ocean University of China,2007,6(3):259-267. |
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| Authors: | Li Qi Xu Yanhong Yu Ruihai Kijima Akihiro |
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| Institution: | 1. College of Fisheries, Ocean University of China, Qingdao 266003, P. R. China
2. Marine Field Station, Field Science Center, Graduate School of Agricultural Science, Tohoku University,Onagawa, Oshika 986-2242, Japan |
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| Abstract: | A genetic linkage map of Pacific abalone(Haliotis discus hannai) was constructed using AFLP markers based on a two-way pseudo-testcross strategy in a full-sib family. With 33 primer combinations,a total of 455 markers(225 from the female parent and 230 from the male parent) segregated in a 1:1 ratio,corresponding to DNA polymorphism:heterozygous in one parent and null in the other. The female framework map consisted of 174 markers distributed in 18 linkage groups,equivalent to the H. discus hannai haploid chromosome number,and spanning a total length of 2031.4 cM,with an average interval of 13.0 cM between adjacent markers. The male framework map consisted of 195 markers mapped on 19 linkage groups,spanning a total length of 2273.4 cM,with an average spacing of 12.9 cM between adjacent markers. The estimated coverage for the framework linkage maps was 81.2% for the female and 82.1% for the male,on the basis of two estimates of genome length. Fifty-two markers(11.4%) remained unlinked. The level of segregation distortion observed in this cross was 20.4%. These linkage maps will serve as a starting point for linkage studies in the Pacific abalone with potential application for marker-assisted selection in breeding programs. |
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| Keywords: | Pacific abalone Haliotis discus hannai AFLP linkage map |
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