| STUDY ON MIXIS POTENTIAL OF ROTIFER RESTING EGGS（ BRACHIONUS PLICATILIS ） WITH DIFFERENT COLLECTION TIMES AND DIFFERENT PRESERVATION PERIODS |
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| 引用本文： | 周利,郑严,相建海.STUDY ON MIXIS POTENTIAL OF ROTIFER RESTING EGGS（ BRACHIONUS PLICATILIS ） WITH DIFFERENT COLLECTION TIMES AND DIFFERENT PRESERVATION PERIODS[J].中国海洋与湖沼学报,2001,19(3):222-227. |
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| 作者姓名： | 周利 郑严 相建海 |
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| 作者单位： | [1]lnstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China [2]InstituteofMolecularAgrobiology,anAffiliateoftheNationalUniversityofSingapore,117604Singapore |
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| 摘 要： | The present study investigated the possible changes in the mixis potential of rotifer resting eggs produced by a single stock of Brachionus plicatilis and collected and preserved annually from 1985 - 1998. Several clones derived from each batch of resting eggs were cultured under the same conditions for 21 days. The percentage of clones appearing resting eggs and the average yield of resting eggs produced from each clone were recorded and statistically analyzed to find the differences between the mixis potential of those resting egg batches. Results showed that different batches of resting eggs had different mictic levels among their descendent clones; but no regular relationship was found between the mixis potential of resting eggs and their collection times/preservation periods. Several internal and external factors that might affect the mixis potential of resting eggs were discussed.
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| 关 键 词： | 受精作用 克隆 统计分析 派生 轮虫 无性繁殖 |
| 收稿时间： | 17 August 2000 |
| 修稿时间： | 27 December 2000 |
Study on mixis potential of rotifer resting eggs (Brachionus plicatilis) with different collection times and different preservation periods |
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| Zhou Li,Zheng Yan,Xiang Jian-Hai.Study on mixis potential of rotifer resting eggs (Brachionus plicatilis) with different collection times and different preservation periods[J].Chinese Journal of Oceanology and Limnology,2001,19(3):222-227. |
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| Authors: | Zhou Li Zheng Yan Xiang Jian-Hai |
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| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China;(2) Institute of Molecular Agrobiology, an Affiliate of the National University of Singapore, 117604, Singapore |
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| Abstract: | The present study investigated the possible changes in the mixis potential of rotifer resting eggs produced by a single stock
ofBrachionus plicatilis and collected and preserved annually from 1985–1998. Several clones derived from each batch of resting eggs were cultured
under the same conditions for 21 days. The percentage of clones appearing resting eggs and the average yield of resting eggs
produced from each clone were recorded and statistically analyzed to find the differences between the mixis potential of those
resting egg batches. Results showed that different batches of resting eggs had different mictic levels among their descendent
clones; but no regular relationship was found between the mixis potential of resting eggs and their collection times/preservation
periods. Several internal and external factors that might affect the mixis potential of resting eggs were discussed.
Contribution No. 4145 from the Institute of Oceanology, Chinese Academy of Sciences.
Project was supported by a grant from the Singapore-China Biotechnology Program of the Institute of Molecular Agrobiology,
an affiliate of the National University of Singapore, and also supported by Marine Aquaculture Center, Agri-food and Veterinary
Authority of Singapore. |
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| Keywords: | Brachionus plicatilis resting eggs mixis potential collection times preservation periods |
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